Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana
Background: SA (Salicylic acid) and JA (Jasmonic acid)/ET (Ethylene) are the defense and growth hormone regulators involved in alleviating the biotic and abiotic stresses. WRKY and NAC genes are contributors to plant resistance due to their active role in SA and JA/ET defense mechanism. Methods: WRK...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-10-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364720302342 |
| _version_ | 1818873679808823296 |
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| author | Hafiz Muhammad Khalid Abbas Aqeel Ahmad Wubei Dong Jingshu Xiang Javaid Iqbal Sajid Ali Waheed Akram Yu-Juan Zhong |
| author_facet | Hafiz Muhammad Khalid Abbas Aqeel Ahmad Wubei Dong Jingshu Xiang Javaid Iqbal Sajid Ali Waheed Akram Yu-Juan Zhong |
| author_sort | Hafiz Muhammad Khalid Abbas |
| collection | DOAJ |
| description | Background: SA (Salicylic acid) and JA (Jasmonic acid)/ET (Ethylene) are the defense and growth hormone regulators involved in alleviating the biotic and abiotic stresses. WRKY and NAC genes are contributors to plant resistance due to their active role in SA and JA/ET defense mechanism. Methods: WRKY and NAC transcription factors (ptHR293 and ptHR759, respectively) were serially selected (by performing cDNA library functional screening, homology analysis, antioxidant enzymes, ROS burst, callose deposition and qRT-PCR analysis) from Pinellia ternata and transformed into Nicotiana benthamiana. Hybrids were generated to analyze the stability of disease resistance. UPLC-QTOF-MS was performed to study the bioactive compounds. Results: Study demonstrated that ptHR293 and ptHR759 had potential to trigger ROS burst and callose deposition in N. benthamiana together with the activation of PR-genes and antioxidant enzymes. In transformed N. benthamiana, relative expression of PR-1a (Pathogenesis related-1a) and PDF1.2 (Plant defensin 1.2) was upregulated upto 21 folds and 4 folds for ptHR293 transformed N. benthamiana. While PR-1a and PDF1.2 exhibited 8 folds and 26 folds upregulation for ptHR759 transformed N. benthamiana. ptHR293 + ptHR759-F1 hybrids also exhibited a significant level of PR-gene expression. Significantly high resistance against Botrytis cinerea without influencing the standard seed germination, root and shoot length of transformed N. benthamiana was also observed. A significant induction of bioactive compounds was also observed in ptHR293 transformed N. benthamiana. Conclusion: Conclusively, heterologous transcription factors, ptHR293 and ptHR759, perform their specific role in the activation of SA and JA/ET mediated defense mechanism. |
| first_indexed | 2024-12-19T12:58:33Z |
| format | Article |
| id | doaj.art-e4a2ae57f8d0431798b21600c88cb8c6 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-19T12:58:33Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-e4a2ae57f8d0431798b21600c88cb8c62022-12-21T20:20:20ZengElsevierJournal of King Saud University: Science1018-36472020-10-0132730053013Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamianaHafiz Muhammad Khalid Abbas0Aqeel Ahmad1Wubei Dong2Jingshu Xiang3Javaid Iqbal4Sajid Ali5Waheed Akram6Yu-Juan Zhong7Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, PR ChinaVegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, PR ChinaDepartment of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, 430070 Hubei Province, PR ChinaDepartment of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, 430070 Hubei Province, PR China; Guizhou Center for Disease Control and Prevention, Guiyang 55004, PR ChinaDepartment of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi ArabiaInstitute of Agricultural Sciences, University of the Punjab, Lahore 54590, PakistanVegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, PR ChinaVegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, PR China; Corresponding author.Background: SA (Salicylic acid) and JA (Jasmonic acid)/ET (Ethylene) are the defense and growth hormone regulators involved in alleviating the biotic and abiotic stresses. WRKY and NAC genes are contributors to plant resistance due to their active role in SA and JA/ET defense mechanism. Methods: WRKY and NAC transcription factors (ptHR293 and ptHR759, respectively) were serially selected (by performing cDNA library functional screening, homology analysis, antioxidant enzymes, ROS burst, callose deposition and qRT-PCR analysis) from Pinellia ternata and transformed into Nicotiana benthamiana. Hybrids were generated to analyze the stability of disease resistance. UPLC-QTOF-MS was performed to study the bioactive compounds. Results: Study demonstrated that ptHR293 and ptHR759 had potential to trigger ROS burst and callose deposition in N. benthamiana together with the activation of PR-genes and antioxidant enzymes. In transformed N. benthamiana, relative expression of PR-1a (Pathogenesis related-1a) and PDF1.2 (Plant defensin 1.2) was upregulated upto 21 folds and 4 folds for ptHR293 transformed N. benthamiana. While PR-1a and PDF1.2 exhibited 8 folds and 26 folds upregulation for ptHR759 transformed N. benthamiana. ptHR293 + ptHR759-F1 hybrids also exhibited a significant level of PR-gene expression. Significantly high resistance against Botrytis cinerea without influencing the standard seed germination, root and shoot length of transformed N. benthamiana was also observed. A significant induction of bioactive compounds was also observed in ptHR293 transformed N. benthamiana. Conclusion: Conclusively, heterologous transcription factors, ptHR293 and ptHR759, perform their specific role in the activation of SA and JA/ET mediated defense mechanism.http://www.sciencedirect.com/science/article/pii/S1018364720302342Hypersensitive responseNicotiana benthamianaTranscription factorWRKY3,4-Dichloromaleimide |
| spellingShingle | Hafiz Muhammad Khalid Abbas Aqeel Ahmad Wubei Dong Jingshu Xiang Javaid Iqbal Sajid Ali Waheed Akram Yu-Juan Zhong Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana Journal of King Saud University: Science Hypersensitive response Nicotiana benthamiana Transcription factor WRKY 3,4-Dichloromaleimide |
| title | Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana |
| title_full | Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana |
| title_fullStr | Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana |
| title_full_unstemmed | Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana |
| title_short | Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana |
| title_sort | heterologous wrky and nac transcription factors triggered resistance in nicotiana benthamiana |
| topic | Hypersensitive response Nicotiana benthamiana Transcription factor WRKY 3,4-Dichloromaleimide |
| url | http://www.sciencedirect.com/science/article/pii/S1018364720302342 |
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