Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant Properties
Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from <i>Moringa...
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MDPI AG
2021-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/4/512 |
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| author | Juliana Cotabarren Santiago Claver Juan Abreu Payrol Javier Garcia-Pardo Walter David Obregón |
| author_facet | Juliana Cotabarren Santiago Claver Juan Abreu Payrol Javier Garcia-Pardo Walter David Obregón |
| author_sort | Juliana Cotabarren |
| collection | DOAJ |
| description | Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from <i>Moringa oleifera</i> (MoPI). MoPI has a molecular mass of 19 kDa and showed an extraordinary physicochemical stability against acidic pHs and high temperatures. Our findings also revealed that MoPI is one of the most potent cysteine protease inhibitors reported to date, with <i>K</i>i and IC<sub>50</sub> values of 2.1 nM and 5.7 nM, respectively. More interestingly, MoPI presents a strong antimicrobial activity against human pathogens such as <i>Enterococcus faecalis</i> and <i>Staphylococcus aureus</i>. In addition, MoPI also showed important anticoagulant activity, which is an unprecedented property for this family of protease inhibitors. These results highlight the pharmaceutical potential of this plant and its derived bioactive molecules. |
| first_indexed | 2024-03-10T12:31:23Z |
| format | Article |
| id | doaj.art-e4a4dfe3a8b24fc384d65c325f4e3b59 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T12:31:23Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj.art-e4a4dfe3a8b24fc384d65c325f4e3b592023-11-21T14:41:47ZengMDPI AGPharmaceutics1999-49232021-04-0113451210.3390/pharmaceutics13040512Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant PropertiesJuliana Cotabarren0Santiago Claver1Juan Abreu Payrol2Javier Garcia-Pardo3Walter David Obregón4Centro de Investigación de Proteínas Vegetales (CIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Buenos Aires B1900, ArgentinaCentro de Investigación de Proteínas Vegetales (CIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Buenos Aires B1900, ArgentinaEscuela Latinoamericana de Medicina, La Habana 19108, CubaInstitut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, SpainCentro de Investigación de Proteínas Vegetales (CIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Buenos Aires B1900, ArgentinaPlant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from <i>Moringa oleifera</i> (MoPI). MoPI has a molecular mass of 19 kDa and showed an extraordinary physicochemical stability against acidic pHs and high temperatures. Our findings also revealed that MoPI is one of the most potent cysteine protease inhibitors reported to date, with <i>K</i>i and IC<sub>50</sub> values of 2.1 nM and 5.7 nM, respectively. More interestingly, MoPI presents a strong antimicrobial activity against human pathogens such as <i>Enterococcus faecalis</i> and <i>Staphylococcus aureus</i>. In addition, MoPI also showed important anticoagulant activity, which is an unprecedented property for this family of protease inhibitors. These results highlight the pharmaceutical potential of this plant and its derived bioactive molecules.https://www.mdpi.com/1999-4923/13/4/512<i>Moringa oleifera</i>proteaseplant protease inhibitorphytocystatinpapain inhibitorantibacterial |
| spellingShingle | Juliana Cotabarren Santiago Claver Juan Abreu Payrol Javier Garcia-Pardo Walter David Obregón Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant Properties Pharmaceutics <i>Moringa oleifera</i> protease plant protease inhibitor phytocystatin papain inhibitor antibacterial |
| title | Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant Properties |
| title_full | Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant Properties |
| title_fullStr | Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant Properties |
| title_full_unstemmed | Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant Properties |
| title_short | Purification and Characterization of a Novel Thermostable Papain Inhibitor from <i>Moringa oleifera</i> with Antimicrobial and Anticoagulant Properties |
| title_sort | purification and characterization of a novel thermostable papain inhibitor from i moringa oleifera i with antimicrobial and anticoagulant properties |
| topic | <i>Moringa oleifera</i> protease plant protease inhibitor phytocystatin papain inhibitor antibacterial |
| url | https://www.mdpi.com/1999-4923/13/4/512 |
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