Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion
ABSTRACT Aiming to alter and/or improve permeation of active compounds in the skin, many strategies have been developed, including biophysical methods. One of the physical absorption techniques, currently known as Cryo Laser Phoresis (CLP), consists of an apparatus that emits radiation on polar or n...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100008&lng=en&tlng=en |
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| author | Marcio Adriano Andréo Iasmin Mimoto Rufino Daniela Cecilia Ortiz de Orue Ubaldo Estela Boaro Herbst Heron Dominguez Torres da Silva Pedro Gonçalves de Oliveira Marcio Ferrari Paulo Cesar Pires Rosa Patrícia Santos Lopes Vânia Rodrigues Leite-Silva |
| author_facet | Marcio Adriano Andréo Iasmin Mimoto Rufino Daniela Cecilia Ortiz de Orue Ubaldo Estela Boaro Herbst Heron Dominguez Torres da Silva Pedro Gonçalves de Oliveira Marcio Ferrari Paulo Cesar Pires Rosa Patrícia Santos Lopes Vânia Rodrigues Leite-Silva |
| author_sort | Marcio Adriano Andréo |
| collection | DOAJ |
| description | ABSTRACT Aiming to alter and/or improve permeation of active compounds in the skin, many strategies have been developed, including biophysical methods. One of the physical absorption techniques, currently known as Cryo Laser Phoresis (CLP), consists of an apparatus that emits radiation on polar or nonpolar molecules of the active substance, resulting in faster penetration when in comparison to the standard topical application. The goal of this work was to evaluate the efficacy of a method that proposes to increase cutaneous permeation of diclofenac sodium by using CLP technique. The influence on permeation was evaluated ex vivo, using Franz cell and human skin obtained from cosmetic surgery. The results were evaluated using statistical methods and data exploratory analysis: clusters, k-means and Principal Component Analysis. The results showed a larger increase in the concentration of diclofenac sodium in the dermis with the use of laser. In all samples (with or without laser application) it was observed that skin surface showed an amount of diclofenac sodium and that there was no active passage to the receptor liquid, suggesting that diclofenac sodium was not absorbed. These results indicate that CLP, when used under the conditions described in this study, is able to increase diclofenac sodium penetration and its retention into deeper layers. |
| first_indexed | 2024-12-21T10:54:07Z |
| format | Article |
| id | doaj.art-e4a63bc2b30146bd82f90f9d4f9b0d42 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-21T10:54:07Z |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-e4a63bc2b30146bd82f90f9d4f9b0d422022-12-21T19:06:34ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-9790521697610.1590/S1984-82502016000100008S1984-82502016000100008Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusionMarcio Adriano AndréoIasmin Mimoto RufinoDaniela Cecilia Ortiz de Orue UbaldoEstela Boaro HerbstHeron Dominguez Torres da SilvaPedro Gonçalves de OliveiraMarcio FerrariPaulo Cesar Pires RosaPatrícia Santos LopesVânia Rodrigues Leite-SilvaABSTRACT Aiming to alter and/or improve permeation of active compounds in the skin, many strategies have been developed, including biophysical methods. One of the physical absorption techniques, currently known as Cryo Laser Phoresis (CLP), consists of an apparatus that emits radiation on polar or nonpolar molecules of the active substance, resulting in faster penetration when in comparison to the standard topical application. The goal of this work was to evaluate the efficacy of a method that proposes to increase cutaneous permeation of diclofenac sodium by using CLP technique. The influence on permeation was evaluated ex vivo, using Franz cell and human skin obtained from cosmetic surgery. The results were evaluated using statistical methods and data exploratory analysis: clusters, k-means and Principal Component Analysis. The results showed a larger increase in the concentration of diclofenac sodium in the dermis with the use of laser. In all samples (with or without laser application) it was observed that skin surface showed an amount of diclofenac sodium and that there was no active passage to the receptor liquid, suggesting that diclofenac sodium was not absorbed. These results indicate that CLP, when used under the conditions described in this study, is able to increase diclofenac sodium penetration and its retention into deeper layers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100008&lng=en&tlng=enCryo Laser Phoresis/dematologic useCutaneous permeationDiclofenac sodium/cutaneous permeationFranz cell. |
| spellingShingle | Marcio Adriano Andréo Iasmin Mimoto Rufino Daniela Cecilia Ortiz de Orue Ubaldo Estela Boaro Herbst Heron Dominguez Torres da Silva Pedro Gonçalves de Oliveira Marcio Ferrari Paulo Cesar Pires Rosa Patrícia Santos Lopes Vânia Rodrigues Leite-Silva Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion Brazilian Journal of Pharmaceutical Sciences Cryo Laser Phoresis/dematologic use Cutaneous permeation Diclofenac sodium/cutaneous permeation Franz cell. |
| title | Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion |
| title_full | Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion |
| title_fullStr | Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion |
| title_full_unstemmed | Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion |
| title_short | Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion |
| title_sort | performance evaluation of cryo laser phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion |
| topic | Cryo Laser Phoresis/dematologic use Cutaneous permeation Diclofenac sodium/cutaneous permeation Franz cell. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100008&lng=en&tlng=en |
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