Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2
Objective To study the effect of angelica polysaccharide on proliferation and apoptosis of rat cardiomyocyte line H9c2. Methods Rat cardiomyocyte H9c2 was divided into a control group (Control group), doxorubicin (ADR) induced H9c2 cell replication dilated cardiomyopathy cardiomyocyte group (ADR g...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-04-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200359.pdf |
| _version_ | 1797365821001957376 |
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| author | YANG Ping, DAI Tian, ZHANG Su-chuan |
| author_facet | YANG Ping, DAI Tian, ZHANG Su-chuan |
| author_sort | YANG Ping, DAI Tian, ZHANG Su-chuan |
| collection | DOAJ |
| description | Objective To study the effect of angelica polysaccharide on proliferation and apoptosis of rat cardiomyocyte line H9c2. Methods Rat cardiomyocyte H9c2 was divided into a control group (Control group), doxorubicin (ADR) induced H9c2 cell replication dilated cardiomyopathy cardiomyocyte group (ADR group), APS intervention (APS group), transfection anti-miR-NC (APS+anti-miR-NC group) and transfected anti-miR-4701-3p(APS+anti-miR-4701-3p group). Flow cytometry was applied to determine apoptosis, Western blot was employed to test B cell lymphoma/leukemia-2(Bcl-2), Bcl-2 related X protein (Bax), nuclear related antigen Ki-67(Ki-67) and proliferation cell nuclear antigen (PCNA) protein expression, enzyme-linked immunoassay (ELISA) method was used to detect B-type brain natriuretic peptide (BNP) level. Cell proliferation was detected by commercially available kit 8(CCK-8), and real-time quantitative PCR (RT-qPCR) was used to analyze the microRNA-4701-3p(miR-4701-3p) expression. Results Adriamycin significantly increased BNP content, apoptosis rate, and Bax protein level in H9c2 cells, and greatly reduced Bcl-2 protein expression(P＜0.05). Angelica polysac-charide obviously increased H9c2 cell viability, Ki-67, PCNA, Bcl-2 protein expression and miR-4701-3p expression after doxorubicin induction, and remarkably reduced cell apoptosis and Bax protein expression(P＜0.05). Knockdown of miR-4701-3p significantly reduced the cell viability, Ki-67, PCNA, and Bcl-2 protein levels of dilated cardiomyopathy H9c2 cells treated with angelica polysaccharide, while significantly increased its apoptosis rate and Bax protein expression(P＜ 0.05). Conclusions Angelica polysaccharide up-regulates the expression of miR-4701-3p, promotes the proliferation of adriamycin-induced rat cardiomyocyte line H9c2. |
| first_indexed | 2024-03-08T16:54:23Z |
| format | Article |
| id | doaj.art-e4a681e66d3e43cda58bcbc66cb01e7f |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:23Z |
| publishDate | 2021-04-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-e4a681e66d3e43cda58bcbc66cb01e7f2024-01-05T03:04:57ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-04-01414551557Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2YANG Ping, DAI Tian, ZHANG Su-chuan0Department of Cardiovascular Medicine, the Affiliated Hospital of Jianghan University (Wuhan Sixth Hospital),Wuhan 430015, ChinaObjective To study the effect of angelica polysaccharide on proliferation and apoptosis of rat cardiomyocyte line H9c2. Methods Rat cardiomyocyte H9c2 was divided into a control group (Control group), doxorubicin (ADR) induced H9c2 cell replication dilated cardiomyopathy cardiomyocyte group (ADR group), APS intervention (APS group), transfection anti-miR-NC (APS+anti-miR-NC group) and transfected anti-miR-4701-3p(APS+anti-miR-4701-3p group). Flow cytometry was applied to determine apoptosis, Western blot was employed to test B cell lymphoma/leukemia-2(Bcl-2), Bcl-2 related X protein (Bax), nuclear related antigen Ki-67(Ki-67) and proliferation cell nuclear antigen (PCNA) protein expression, enzyme-linked immunoassay (ELISA) method was used to detect B-type brain natriuretic peptide (BNP) level. Cell proliferation was detected by commercially available kit 8(CCK-8), and real-time quantitative PCR (RT-qPCR) was used to analyze the microRNA-4701-3p(miR-4701-3p) expression. Results Adriamycin significantly increased BNP content, apoptosis rate, and Bax protein level in H9c2 cells, and greatly reduced Bcl-2 protein expression(P＜0.05). Angelica polysac-charide obviously increased H9c2 cell viability, Ki-67, PCNA, Bcl-2 protein expression and miR-4701-3p expression after doxorubicin induction, and remarkably reduced cell apoptosis and Bax protein expression(P＜0.05). Knockdown of miR-4701-3p significantly reduced the cell viability, Ki-67, PCNA, and Bcl-2 protein levels of dilated cardiomyopathy H9c2 cells treated with angelica polysaccharide, while significantly increased its apoptosis rate and Bax protein expression(P＜ 0.05). Conclusions Angelica polysaccharide up-regulates the expression of miR-4701-3p, promotes the proliferation of adriamycin-induced rat cardiomyocyte line H9c2.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200359.pdfadriamycin|cell line h9c2|mir-4701-3p|bnp |
| spellingShingle | YANG Ping, DAI Tian, ZHANG Su-chuan Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2 Jichu yixue yu linchuang adriamycin|cell line h9c2|mir-4701-3p|bnp |
| title | Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2 |
| title_full | Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2 |
| title_fullStr | Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2 |
| title_full_unstemmed | Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2 |
| title_short | Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2 |
| title_sort | angelica polysaccharide promotes the proliferation of rat cardiomyocyte line h9c2 |
| topic | adriamycin|cell line h9c2|mir-4701-3p|bnp |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200359.pdf |
| work_keys_str_mv | AT yangpingdaitianzhangsuchuan angelicapolysaccharidepromotestheproliferationofratcardiomyocytelineh9c2 |