Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct Metabolism
The control of <i>P. xylostella</i> primarily involves chemical insecticides, but overuse has brought about many negative effects. Our previous study reported that (S)-(−)-palasonin (PLN) is a plant-derived active substance with significant insecticidal activity against <i>P. xylos...
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MDPI AG
2022-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/11/989 |
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| author | Qiqi Fan Jiyuan Liu Yifan Li Yalin Zhang |
| author_facet | Qiqi Fan Jiyuan Liu Yifan Li Yalin Zhang |
| author_sort | Qiqi Fan |
| collection | DOAJ |
| description | The control of <i>P. xylostella</i> primarily involves chemical insecticides, but overuse has brought about many negative effects. Our previous study reported that (S)-(−)-palasonin (PLN) is a plant-derived active substance with significant insecticidal activity against <i>P. xylostella</i>. However, we noticed a possible cross-resistance between (S)-(−)-palasonin and other insecticides which may be related to metabolic detoxification. In order to further explore the detoxification effect of detoxification enzymes on (S)-(−)-palasonin in <i>P. xylostella</i>, the effects of (S)-(−)-palasonin on enzyme activity and transcription level were determined, and the detoxification and metabolism of GSTs on (S)-(−)-palasonin were studied by in vitro inhibition and metabolism experiments. During this study, GST enzyme activity was significantly increased in <i>P. xylostella</i> after (S)-(−)-palasonin treatment. The expression levels of 19 GSTs genes were significantly increased whereas the expression levels of 1 gene decreased. Furthermore, (S)-(−)-palasonin is shown to be stabilized with GSTs and metabolized GSTs (GSTd1, GSTd2, GSTs1 and GSTs2) in vitro, with the highest metabolic rate of 80.59% for GSTs1. This study advances the beneficial utilization of (S)-(−)-palasonin as a botanical pesticide to control <i>P. xylostella</i> in the field. |
| first_indexed | 2024-03-09T18:59:08Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T18:59:08Z |
| publishDate | 2022-10-01 |
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| series | Insects |
| spelling | doaj.art-e4a8f63212ae4640a530ff6366115c9b2023-11-24T05:13:06ZengMDPI AGInsects2075-44502022-10-01131198910.3390/insects13110989Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct MetabolismQiqi Fan0Jiyuan Liu1Yifan Li2Yalin Zhang3Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaThe control of <i>P. xylostella</i> primarily involves chemical insecticides, but overuse has brought about many negative effects. Our previous study reported that (S)-(−)-palasonin (PLN) is a plant-derived active substance with significant insecticidal activity against <i>P. xylostella</i>. However, we noticed a possible cross-resistance between (S)-(−)-palasonin and other insecticides which may be related to metabolic detoxification. In order to further explore the detoxification effect of detoxification enzymes on (S)-(−)-palasonin in <i>P. xylostella</i>, the effects of (S)-(−)-palasonin on enzyme activity and transcription level were determined, and the detoxification and metabolism of GSTs on (S)-(−)-palasonin were studied by in vitro inhibition and metabolism experiments. During this study, GST enzyme activity was significantly increased in <i>P. xylostella</i> after (S)-(−)-palasonin treatment. The expression levels of 19 GSTs genes were significantly increased whereas the expression levels of 1 gene decreased. Furthermore, (S)-(−)-palasonin is shown to be stabilized with GSTs and metabolized GSTs (GSTd1, GSTd2, GSTs1 and GSTs2) in vitro, with the highest metabolic rate of 80.59% for GSTs1. This study advances the beneficial utilization of (S)-(−)-palasonin as a botanical pesticide to control <i>P. xylostella</i> in the field.https://www.mdpi.com/2075-4450/13/11/989<i>Plutella xylostella</i> (L.)(S)-(−)-palasoninbotanical insecticidemetabolism |
| spellingShingle | Qiqi Fan Jiyuan Liu Yifan Li Yalin Zhang Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct Metabolism Insects <i>Plutella xylostella</i> (L.) (S)-(−)-palasonin botanical insecticide metabolism |
| title | Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct Metabolism |
| title_full | Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct Metabolism |
| title_fullStr | Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct Metabolism |
| title_full_unstemmed | Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct Metabolism |
| title_short | Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in <i>Plutella xylostella</i> (L.) via Direct Metabolism |
| title_sort | glutathione s transferase may contribute to the detoxification of s palasonin in i plutella xylostella i l via direct metabolism |
| topic | <i>Plutella xylostella</i> (L.) (S)-(−)-palasonin botanical insecticide metabolism |
| url | https://www.mdpi.com/2075-4450/13/11/989 |
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