Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was...
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MDPI AG
2019-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/11/664 |
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| author | Lucas Pires Guarnier Paulo Vitor Moreira Romão Rhanany Alan Calloi Palozi Aniely Oliveira Silva Bethânia Rosa Lorençone Aline Aparecida Macedo Marques Ariany Carvalho dos Santos Roosevelt Isaias Carvalho Souza Karine Delgado Souza Emerson Luiz Botelho Lourenço Arquimedes Gasparotto Junior |
| author_facet | Lucas Pires Guarnier Paulo Vitor Moreira Romão Rhanany Alan Calloi Palozi Aniely Oliveira Silva Bethânia Rosa Lorençone Aline Aparecida Macedo Marques Ariany Carvalho dos Santos Roosevelt Isaias Carvalho Souza Karine Delgado Souza Emerson Luiz Botelho Lourenço Arquimedes Gasparotto Junior |
| author_sort | Lucas Pires Guarnier |
| collection | DOAJ |
| description | Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-<span style="font-variant: small-caps;">l</span>-arginine methyl ester hydrochloride (<span style="font-variant: small-caps;">l</span>-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral <span style="font-variant: small-caps;">l</span>-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats. |
| first_indexed | 2024-12-13T21:18:14Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-13T21:18:14Z |
| publishDate | 2019-10-01 |
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| spelling | doaj.art-e4a91c2dca6744cea4cf538aabff7ca32022-12-21T23:31:12ZengMDPI AGBiomolecules2218-273X2019-10-0191166410.3390/biom9110664biom9110664Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female RatsLucas Pires Guarnier0Paulo Vitor Moreira Romão1Rhanany Alan Calloi Palozi2Aniely Oliveira Silva3Bethânia Rosa Lorençone4Aline Aparecida Macedo Marques5Ariany Carvalho dos Santos6Roosevelt Isaias Carvalho Souza7Karine Delgado Souza8Emerson Luiz Botelho Lourenço9Arquimedes Gasparotto Junior10Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University (UNIPAR), Umuarama, PR 87502-210, BrazilLaboratory of Preclinical Research of Natural Products, Paranaense University (UNIPAR), Umuarama, PR 87502-210, BrazilLaboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, BrazilTherapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-<span style="font-variant: small-caps;">l</span>-arginine methyl ester hydrochloride (<span style="font-variant: small-caps;">l</span>-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral <span style="font-variant: small-caps;">l</span>-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats.https://www.mdpi.com/2218-273X/9/11/664atherosclerosisdyslipidemiahepatic steatosiskidney failure |
| spellingShingle | Lucas Pires Guarnier Paulo Vitor Moreira Romão Rhanany Alan Calloi Palozi Aniely Oliveira Silva Bethânia Rosa Lorençone Aline Aparecida Macedo Marques Ariany Carvalho dos Santos Roosevelt Isaias Carvalho Souza Karine Delgado Souza Emerson Luiz Botelho Lourenço Arquimedes Gasparotto Junior Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats Biomolecules atherosclerosis dyslipidemia hepatic steatosis kidney failure |
| title | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
| title_full | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
| title_fullStr | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
| title_full_unstemmed | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
| title_short | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
| title_sort | development of a predictive model to induce atherogenesis and hepato renal impairment in female rats |
| topic | atherosclerosis dyslipidemia hepatic steatosis kidney failure |
| url | https://www.mdpi.com/2218-273X/9/11/664 |
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