Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat Straw
Ferulic acid esterases belong to the category of carboxylesterases and possess the capability to enzymatically break down hemicellulose within lignocellulosic substances, thereby liberating ferulic acid. A ferulic acid esterase from <i>Lentinula edodes</i> (LeFae) was expressed using <...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/9/7/683 |
| _version_ | 1827733092215816192 |
|---|---|
| author | Xiangyu Zhang Xiaowen Lei Kehui Ouyang Wenjing Zhang Chanjuan Liu Yanjiao Li Qinghua Qiu Yitian Zang Mingren Qu Ke Pan Xianghui Zhao |
| author_facet | Xiangyu Zhang Xiaowen Lei Kehui Ouyang Wenjing Zhang Chanjuan Liu Yanjiao Li Qinghua Qiu Yitian Zang Mingren Qu Ke Pan Xianghui Zhao |
| author_sort | Xiangyu Zhang |
| collection | DOAJ |
| description | Ferulic acid esterases belong to the category of carboxylesterases and possess the capability to enzymatically break down hemicellulose within lignocellulosic substances, thereby liberating ferulic acid. A ferulic acid esterase from <i>Lentinula edodes</i> (LeFae) was expressed using <i>Pichia pastoris</i>, and its characterization and effects on the in vitro fermentation of wheat straw were investigated in this study. The optimal pH and temperature for LeFae were pH 7.0 and 60 °C, respectively. LeFae exhibited a broad temperature and pH adaptability (>60% of the maximum activity at pH 4.0–7.0 and 40–70 °C) and excellent thermal stability. The activity of LeFae was increased by 30.3% with a dosage of Tween 20 at 0.25% (<i>v</i>/<i>v</i>) and exhibited satisfactory resistance to Mn<sup>2+</sup> and sodium dodecyl sulfate. LeFae released ferulic acid from wheat straw and exhibited an obvious synergistic effect with cellulase during wheat straw hydrolysis. LeFae severely inhibited the microbial fermentation of wheat straw and reduced the in vitro dry matter digestibility, total volatile fatty acid yield, and 16S rDNA copy numbers of <i>Ruminococcus flavefaciens</i> by 9.6%, 9.9 mM, and 40.1%, respectively. It also increased pH and the concentration of soluble phenols during wheat straw fermentation. Pretreating wheat straw with LeFae did not affect the microbial fermentation of wheat straw but resulted in the leaching of more dissolving sugars. The current results showed that although LeFae can cooperate with cellulase to promote the hydrolysis of wheat straw, its adverse effect on rumen microorganisms when directly fed to ruminants is a problem worthy of consideration. |
| first_indexed | 2024-03-11T01:06:09Z |
| format | Article |
| id | doaj.art-e4ab64b06ec645dc9f9560202821999c |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:06:09Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-e4ab64b06ec645dc9f9560202821999c2023-11-18T19:16:57ZengMDPI AGFermentation2311-56372023-07-019768310.3390/fermentation9070683Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat StrawXiangyu Zhang0Xiaowen Lei1Kehui Ouyang2Wenjing Zhang3Chanjuan Liu4Yanjiao Li5Qinghua Qiu6Yitian Zang7Mingren Qu8Ke Pan9Xianghui Zhao10Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaGanzhou Animal Husbandry and Fisheries Research Institute, Gannan Academy of Sciences, Ganzhou 341000, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, ChinaFerulic acid esterases belong to the category of carboxylesterases and possess the capability to enzymatically break down hemicellulose within lignocellulosic substances, thereby liberating ferulic acid. A ferulic acid esterase from <i>Lentinula edodes</i> (LeFae) was expressed using <i>Pichia pastoris</i>, and its characterization and effects on the in vitro fermentation of wheat straw were investigated in this study. The optimal pH and temperature for LeFae were pH 7.0 and 60 °C, respectively. LeFae exhibited a broad temperature and pH adaptability (>60% of the maximum activity at pH 4.0–7.0 and 40–70 °C) and excellent thermal stability. The activity of LeFae was increased by 30.3% with a dosage of Tween 20 at 0.25% (<i>v</i>/<i>v</i>) and exhibited satisfactory resistance to Mn<sup>2+</sup> and sodium dodecyl sulfate. LeFae released ferulic acid from wheat straw and exhibited an obvious synergistic effect with cellulase during wheat straw hydrolysis. LeFae severely inhibited the microbial fermentation of wheat straw and reduced the in vitro dry matter digestibility, total volatile fatty acid yield, and 16S rDNA copy numbers of <i>Ruminococcus flavefaciens</i> by 9.6%, 9.9 mM, and 40.1%, respectively. It also increased pH and the concentration of soluble phenols during wheat straw fermentation. Pretreating wheat straw with LeFae did not affect the microbial fermentation of wheat straw but resulted in the leaching of more dissolving sugars. The current results showed that although LeFae can cooperate with cellulase to promote the hydrolysis of wheat straw, its adverse effect on rumen microorganisms when directly fed to ruminants is a problem worthy of consideration.https://www.mdpi.com/2311-5637/9/7/683ferulic acid esterasewheat strawhydrolysismicrobial fermentation |
| spellingShingle | Xiangyu Zhang Xiaowen Lei Kehui Ouyang Wenjing Zhang Chanjuan Liu Yanjiao Li Qinghua Qiu Yitian Zang Mingren Qu Ke Pan Xianghui Zhao Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat Straw Fermentation ferulic acid esterase wheat straw hydrolysis microbial fermentation |
| title | Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat Straw |
| title_full | Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat Straw |
| title_fullStr | Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat Straw |
| title_full_unstemmed | Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat Straw |
| title_short | Characteristics of a Recombinant <i>Lentinula edodes</i> Ferulic Acid Esterase and Its Adverse Effects on In Vitro Fermentation of Wheat Straw |
| title_sort | characteristics of a recombinant i lentinula edodes i ferulic acid esterase and its adverse effects on in vitro fermentation of wheat straw |
| topic | ferulic acid esterase wheat straw hydrolysis microbial fermentation |
| url | https://www.mdpi.com/2311-5637/9/7/683 |
| work_keys_str_mv | AT xiangyuzhang characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT xiaowenlei characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT kehuiouyang characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT wenjingzhang characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT chanjuanliu characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT yanjiaoli characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT qinghuaqiu characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT yitianzang characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT mingrenqu characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT kepan characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw AT xianghuizhao characteristicsofarecombinantilentinulaedodesiferulicacidesteraseanditsadverseeffectsoninvitrofermentationofwheatstraw |