Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolism
Ulcerative colitis (UC) has been confirmed as a disease with a high incidence and low cure rate worldwide. In severe cases, UC can develop into colon cancer. Modern research has confirmed that berberine (BBR) can treat UC by inhibiting the expressions of inflammatory factors. However, the contributi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223012283 |
| _version_ | 1797660610627895296 |
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| author | Xiongjie Sun Yu Zhang Gang Cheng Tianxiang Zhu Zhigang Zhang Lei Xiong Haiming Hu Hongtao Liu |
| author_facet | Xiongjie Sun Yu Zhang Gang Cheng Tianxiang Zhu Zhigang Zhang Lei Xiong Haiming Hu Hongtao Liu |
| author_sort | Xiongjie Sun |
| collection | DOAJ |
| description | Ulcerative colitis (UC) has been confirmed as a disease with a high incidence and low cure rate worldwide. In severe cases, UC can develop into colon cancer. Modern research has confirmed that berberine (BBR) can treat UC by inhibiting the expressions of inflammatory factors. However, the contribution of gut microbiota and flora metabolites in treating UC with BBR remains unclear. In this study, the ameliorative effects of BBR on gut microbiota dysbiosis and flora metabolites were investigated in a dextran sodium sulfate (DSS)-induced UC rodent model. We found that BBR significantly improved the pathological phenotype, attenuated intestinal barrier disruption, and mitigated colonic inflammation in DSS mice. By 16 S rDNA sequencing, BBR alleviated gut microbiota dysbiosis in UC mice. Moreover, the gut microbiota depletion experiment confirmed that the therapeutic effect of BBR was inextricably correlated with the gut microbiota. Besides, the flora metabolites (e.g., short-chain fatty acids, bile acids, and 5-hydroxytryptamine) were studied using HPLC-MS. The results suggested that BBR ameliorated the bile acid imbalance induced by DSS in the liver and gut. Furthermore, BBR treatment repaired gut barrier damage. The above results revealed that BBR alleviated DSS-induced UC in mice by restoring the disturbed gut microbiota, elevating unconjugated and secondary bile acids in the gastrointestinal tract, and activating the FXR and TGR5 signal pathway. This study provides novel insights into the mechanism of BBR in treating UC. |
| first_indexed | 2024-03-11T18:32:22Z |
| format | Article |
| id | doaj.art-e4ae01dd5b2f4a1fa7a24b0fa0286195 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-11T18:32:22Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-e4ae01dd5b2f4a1fa7a24b0fa02861952023-10-13T11:02:36ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-11-01167115430Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolismXiongjie Sun0Yu Zhang1Gang Cheng2Tianxiang Zhu3Zhigang Zhang4Lei Xiong5Haiming Hu6Hongtao Liu7College of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; School of Pharmacy, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaCollege of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; School of Pharmacy, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaHubei Provincial Hospital of Traditional Chinese Medicine, Hubei University of Chinese Medicine, Wuhan 430061, PR ChinaCollege of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaCollege of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaCollege of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; Corresponding author.College of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; School of Pharmacy, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; Corresponding author at: College of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China.Ulcerative colitis (UC) has been confirmed as a disease with a high incidence and low cure rate worldwide. In severe cases, UC can develop into colon cancer. Modern research has confirmed that berberine (BBR) can treat UC by inhibiting the expressions of inflammatory factors. However, the contribution of gut microbiota and flora metabolites in treating UC with BBR remains unclear. In this study, the ameliorative effects of BBR on gut microbiota dysbiosis and flora metabolites were investigated in a dextran sodium sulfate (DSS)-induced UC rodent model. We found that BBR significantly improved the pathological phenotype, attenuated intestinal barrier disruption, and mitigated colonic inflammation in DSS mice. By 16 S rDNA sequencing, BBR alleviated gut microbiota dysbiosis in UC mice. Moreover, the gut microbiota depletion experiment confirmed that the therapeutic effect of BBR was inextricably correlated with the gut microbiota. Besides, the flora metabolites (e.g., short-chain fatty acids, bile acids, and 5-hydroxytryptamine) were studied using HPLC-MS. The results suggested that BBR ameliorated the bile acid imbalance induced by DSS in the liver and gut. Furthermore, BBR treatment repaired gut barrier damage. The above results revealed that BBR alleviated DSS-induced UC in mice by restoring the disturbed gut microbiota, elevating unconjugated and secondary bile acids in the gastrointestinal tract, and activating the FXR and TGR5 signal pathway. This study provides novel insights into the mechanism of BBR in treating UC.http://www.sciencedirect.com/science/article/pii/S0753332223012283BerberineUlcerative colitisGut microbiotaIntestinal metabolites, Bile acids |
| spellingShingle | Xiongjie Sun Yu Zhang Gang Cheng Tianxiang Zhu Zhigang Zhang Lei Xiong Haiming Hu Hongtao Liu Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolism Biomedicine & Pharmacotherapy Berberine Ulcerative colitis Gut microbiota Intestinal metabolites, Bile acids |
| title | Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolism |
| title_full | Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolism |
| title_fullStr | Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolism |
| title_full_unstemmed | Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolism |
| title_short | Berberine improves DSS-induced colitis in mice by modulating the fecal-bacteria-related bile acid metabolism |
| title_sort | berberine improves dss induced colitis in mice by modulating the fecal bacteria related bile acid metabolism |
| topic | Berberine Ulcerative colitis Gut microbiota Intestinal metabolites, Bile acids |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223012283 |
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