Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals
The developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling p...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1422-0067/20/5/1065 |
| _version_ | 1811343444452311040 |
|---|---|
| author | Elisabet Teixidó Marta Barenys Ester Piqué Joan M. Llobet Jesús Gómez-Catalán |
| author_facet | Elisabet Teixidó Marta Barenys Ester Piqué Joan M. Llobet Jesús Gómez-Catalán |
| author_sort | Elisabet Teixidó |
| collection | DOAJ |
| description | The developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling pathways. Therefore, we exposed zebrafish embryos to PCB126 or to several redox-modulating chemicals to study their ability to modulate the dysmorphogenesis produced by PCB126. PCB126 produced a concentration-dependent induction of pericardial edema and circulatory failure, and a concentration-dependent reduction of cardiac output and body length at 80 hours post fertilization (hpf). Among several modulators tested, the effects of PCB126 could be both positively and negatively modulated by different compounds; co-treatment with α-tocopherol (vitamin E liposoluble) prevented the adverse effects of PCB126 in pericardial edema, whereas co-treatment with sodium nitroprusside (a vasodilator compound) significantly worsened PCB126 effects. Gene expression analysis showed an up-regulation of cyp1a, hsp70, and gstp1, indicative of PCB126 interaction with the aryl hydrocarbon receptor (AhR), while the transcription of antioxidant genes (sod1, sod2; cat and gpx1a) was not affected. Further studies are necessary to understand the role of oxidative stress in the developmental toxicity of low concentrations of PCB126 (25 nM). Our results give insights into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of environmental pollutants. |
| first_indexed | 2024-04-13T19:30:33Z |
| format | Article |
| id | doaj.art-e4b3cc576fed45fab5456c4407e66804 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T19:30:33Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e4b3cc576fed45fab5456c4407e668042022-12-22T02:33:13ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-03-01205106510.3390/ijms20051065ijms20051065Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating ChemicalsElisabet Teixidó0Marta Barenys1Ester Piqué2Joan M. Llobet3Jesús Gómez-Catalán4Department of Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research—UFZ, Permoserstraße 15, 04318 Leipzig, GermanyGRET-Toxicology Unit, Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, SpainGRET-Toxicology Unit, Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, SpainGRET-Toxicology Unit, Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, SpainGRET-Toxicology Unit, Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, SpainThe developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling pathways. Therefore, we exposed zebrafish embryos to PCB126 or to several redox-modulating chemicals to study their ability to modulate the dysmorphogenesis produced by PCB126. PCB126 produced a concentration-dependent induction of pericardial edema and circulatory failure, and a concentration-dependent reduction of cardiac output and body length at 80 hours post fertilization (hpf). Among several modulators tested, the effects of PCB126 could be both positively and negatively modulated by different compounds; co-treatment with α-tocopherol (vitamin E liposoluble) prevented the adverse effects of PCB126 in pericardial edema, whereas co-treatment with sodium nitroprusside (a vasodilator compound) significantly worsened PCB126 effects. Gene expression analysis showed an up-regulation of cyp1a, hsp70, and gstp1, indicative of PCB126 interaction with the aryl hydrocarbon receptor (AhR), while the transcription of antioxidant genes (sod1, sod2; cat and gpx1a) was not affected. Further studies are necessary to understand the role of oxidative stress in the developmental toxicity of low concentrations of PCB126 (25 nM). Our results give insights into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of environmental pollutants.http://www.mdpi.com/1422-0067/20/5/1065cardiovascular toxicityphenotypezebrafish embryoredox modulatorsqPCR |
| spellingShingle | Elisabet Teixidó Marta Barenys Ester Piqué Joan M. Llobet Jesús Gómez-Catalán Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals International Journal of Molecular Sciences cardiovascular toxicity phenotype zebrafish embryo redox modulators qPCR |
| title | Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals |
| title_full | Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals |
| title_fullStr | Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals |
| title_full_unstemmed | Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals |
| title_short | Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals |
| title_sort | cardiovascular effects of pcb 126 3 3 4 4 5 pentachlorobiphenyl in zebrafish embryos and impact of co exposure to redox modulating chemicals |
| topic | cardiovascular toxicity phenotype zebrafish embryo redox modulators qPCR |
| url | http://www.mdpi.com/1422-0067/20/5/1065 |
| work_keys_str_mv | AT elisabetteixido cardiovasculareffectsofpcb12633445pentachlorobiphenylinzebrafishembryosandimpactofcoexposuretoredoxmodulatingchemicals AT martabarenys cardiovasculareffectsofpcb12633445pentachlorobiphenylinzebrafishembryosandimpactofcoexposuretoredoxmodulatingchemicals AT esterpique cardiovasculareffectsofpcb12633445pentachlorobiphenylinzebrafishembryosandimpactofcoexposuretoredoxmodulatingchemicals AT joanmllobet cardiovasculareffectsofpcb12633445pentachlorobiphenylinzebrafishembryosandimpactofcoexposuretoredoxmodulatingchemicals AT jesusgomezcatalan cardiovasculareffectsofpcb12633445pentachlorobiphenylinzebrafishembryosandimpactofcoexposuretoredoxmodulatingchemicals |