Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing
Biocompatibility and biomineralization of root canal dressings are important requirements for periapical healing. This study evaluated the inflammatory response, biomineralization and tissue repair by collagen fiber maturation in the subcutaneous tissue of rats. Eighteen Wistar rats (<i>n</...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/14/10/507 |
| _version_ | 1827720786834620416 |
|---|---|
| author | Carlos Roberto Emerenciano Bueno Jimena Lama Sarmiento Ana Maria Veiga Vasques Ana Cláudia Rodrigues da Silva Luciano Tavares Angelo Cintra João Miguel Marques Santos Eloi Dezan-Júnior |
| author_facet | Carlos Roberto Emerenciano Bueno Jimena Lama Sarmiento Ana Maria Veiga Vasques Ana Cláudia Rodrigues da Silva Luciano Tavares Angelo Cintra João Miguel Marques Santos Eloi Dezan-Júnior |
| author_sort | Carlos Roberto Emerenciano Bueno |
| collection | DOAJ |
| description | Biocompatibility and biomineralization of root canal dressings are important requirements for periapical healing. This study evaluated the inflammatory response, biomineralization and tissue repair by collagen fiber maturation in the subcutaneous tissue of rats. Eighteen Wistar rats (<i>n</i> = 6) received subcutaneous implants: calcium hydroxide + propylene glycol [CH+P], calcium hydroxide + propylene glycol + iodoform [CH+P+I], iodoform + carbowax [I+Cwax] and carbowax [Cwax]. Extra empty tubes were used as a control [C]. After 7, 15 and 30 days, the implants were removed with surrounding tissue for staining of hematoxylin-eosin, Von Kossa, picrosirius red and without staining for analysis under polarized light. Results were analyzed via Kruskal–Wallis followed by Dunn testing for nonparametric data and ANOVA followed by a Tukey post hoc test for parametric data (<i>p</i> < 5%). At 7 days, all groups showed a moderate inflammatory reaction and thick fibrous capsule, except the [Cwax] group, with a severe inflammatory infiltrate (<i>p</i> < 0.05). After 15 days, all groups but control had a decrease in inflammatory response. At 30 days, all groups presented a mild reaction and thin fibrous capsule (<i>p</i> > 0.05). Only groups containing calcium hydroxide were found to be positive using Von Kossa staining and polarized light in all periods. At 7 days, all groups showed a higher proportion of immature fibers. At 15 days, the [CH+P] and [Cwax] groups increased their proportion of mature/immature fibers. At 30 days, only the [CH+P] group presented a significant prevalence of mature collagen fibers (<i>p</i> < 0.05). All groups showed biocompatibility, but only groups containing calcium hydroxide induced biomineralization. The addition of iodoform delayed tissue healing. |
| first_indexed | 2024-03-10T21:10:19Z |
| format | Article |
| id | doaj.art-e4b9e8f95eaa428abbb74507dbb35491 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T21:10:19Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-e4b9e8f95eaa428abbb74507dbb354912023-11-19T16:55:42ZengMDPI AGJournal of Functional Biomaterials2079-49832023-10-01141050710.3390/jfb14100507Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal DressingCarlos Roberto Emerenciano Bueno0Jimena Lama Sarmiento1Ana Maria Veiga Vasques2Ana Cláudia Rodrigues da Silva3Luciano Tavares Angelo Cintra4João Miguel Marques Santos5Eloi Dezan-Júnior6Endodontic Section, Department of Preventive and Restorative Dentistry, Araçatuba School of Dentistry (FOA), São Paulo State University (Unesp), Araçatuba 16015-050, SP, BrazilEndodontic Section, Department of Preventive and Restorative Dentistry, Araçatuba School of Dentistry (FOA), São Paulo State University (Unesp), Araçatuba 16015-050, SP, BrazilEndodontic Section, Department of Preventive and Restorative Dentistry, Araçatuba School of Dentistry (FOA), São Paulo State University (Unesp), Araçatuba 16015-050, SP, BrazilEndodontic Section, Department of Preventive and Restorative Dentistry, Araçatuba School of Dentistry (FOA), São Paulo State University (Unesp), Araçatuba 16015-050, SP, BrazilEndodontic Section, Department of Preventive and Restorative Dentistry, Araçatuba School of Dentistry (FOA), São Paulo State University (Unesp), Araçatuba 16015-050, SP, BrazilInstitute of Endodontics, Faculty of Medicine, University of Coimbra, 3004-531 Coimbra, PortugalEndodontic Section, Department of Preventive and Restorative Dentistry, Araçatuba School of Dentistry (FOA), São Paulo State University (Unesp), Araçatuba 16015-050, SP, BrazilBiocompatibility and biomineralization of root canal dressings are important requirements for periapical healing. This study evaluated the inflammatory response, biomineralization and tissue repair by collagen fiber maturation in the subcutaneous tissue of rats. Eighteen Wistar rats (<i>n</i> = 6) received subcutaneous implants: calcium hydroxide + propylene glycol [CH+P], calcium hydroxide + propylene glycol + iodoform [CH+P+I], iodoform + carbowax [I+Cwax] and carbowax [Cwax]. Extra empty tubes were used as a control [C]. After 7, 15 and 30 days, the implants were removed with surrounding tissue for staining of hematoxylin-eosin, Von Kossa, picrosirius red and without staining for analysis under polarized light. Results were analyzed via Kruskal–Wallis followed by Dunn testing for nonparametric data and ANOVA followed by a Tukey post hoc test for parametric data (<i>p</i> < 5%). At 7 days, all groups showed a moderate inflammatory reaction and thick fibrous capsule, except the [Cwax] group, with a severe inflammatory infiltrate (<i>p</i> < 0.05). After 15 days, all groups but control had a decrease in inflammatory response. At 30 days, all groups presented a mild reaction and thin fibrous capsule (<i>p</i> > 0.05). Only groups containing calcium hydroxide were found to be positive using Von Kossa staining and polarized light in all periods. At 7 days, all groups showed a higher proportion of immature fibers. At 15 days, the [CH+P] and [Cwax] groups increased their proportion of mature/immature fibers. At 30 days, only the [CH+P] group presented a significant prevalence of mature collagen fibers (<i>p</i> < 0.05). All groups showed biocompatibility, but only groups containing calcium hydroxide induced biomineralization. The addition of iodoform delayed tissue healing.https://www.mdpi.com/2079-4983/14/10/507calcium hydroxidecollagenendodonticsinflammationiodoformintracanal dressing |
| spellingShingle | Carlos Roberto Emerenciano Bueno Jimena Lama Sarmiento Ana Maria Veiga Vasques Ana Cláudia Rodrigues da Silva Luciano Tavares Angelo Cintra João Miguel Marques Santos Eloi Dezan-Júnior Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing Journal of Functional Biomaterials calcium hydroxide collagen endodontics inflammation iodoform intracanal dressing |
| title | Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing |
| title_full | Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing |
| title_fullStr | Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing |
| title_full_unstemmed | Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing |
| title_short | Biocompatibility, Biomineralization and Induction of Collagen Maturation with the Use of Calcium Hydroxide and Iodoform Intracanal Dressing |
| title_sort | biocompatibility biomineralization and induction of collagen maturation with the use of calcium hydroxide and iodoform intracanal dressing |
| topic | calcium hydroxide collagen endodontics inflammation iodoform intracanal dressing |
| url | https://www.mdpi.com/2079-4983/14/10/507 |
| work_keys_str_mv | AT carlosrobertoemerencianobueno biocompatibilitybiomineralizationandinductionofcollagenmaturationwiththeuseofcalciumhydroxideandiodoformintracanaldressing AT jimenalamasarmiento biocompatibilitybiomineralizationandinductionofcollagenmaturationwiththeuseofcalciumhydroxideandiodoformintracanaldressing AT anamariaveigavasques biocompatibilitybiomineralizationandinductionofcollagenmaturationwiththeuseofcalciumhydroxideandiodoformintracanaldressing AT anaclaudiarodriguesdasilva biocompatibilitybiomineralizationandinductionofcollagenmaturationwiththeuseofcalciumhydroxideandiodoformintracanaldressing AT lucianotavaresangelocintra biocompatibilitybiomineralizationandinductionofcollagenmaturationwiththeuseofcalciumhydroxideandiodoformintracanaldressing AT joaomiguelmarquessantos biocompatibilitybiomineralizationandinductionofcollagenmaturationwiththeuseofcalciumhydroxideandiodoformintracanaldressing AT eloidezanjunior biocompatibilitybiomineralizationandinductionofcollagenmaturationwiththeuseofcalciumhydroxideandiodoformintracanaldressing |