AePUb promoter length modulates gene expression in Aedes aegypti
Abstract Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47777-3 |
| _version_ | 1797452685412139008 |
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| author | Michelle A. E. Anderson Philip T. Leftwich Ray Wilson Leonela Z. Carabajal Paladino Sanjay Basu Sara Rooney Zach N. Adelman Luke Alphey |
| author_facet | Michelle A. E. Anderson Philip T. Leftwich Ray Wilson Leonela Z. Carabajal Paladino Sanjay Basu Sara Rooney Zach N. Adelman Luke Alphey |
| author_sort | Michelle A. E. Anderson |
| collection | DOAJ |
| description | Abstract Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects. |
| first_indexed | 2024-03-09T15:12:13Z |
| format | Article |
| id | doaj.art-e4baf41570014ae0bae6694211dc1f2d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:12:13Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e4baf41570014ae0bae6694211dc1f2d2023-11-26T13:21:12ZengNature PortfolioScientific Reports2045-23222023-11-011311710.1038/s41598-023-47777-3AePUb promoter length modulates gene expression in Aedes aegyptiMichelle A. E. Anderson0Philip T. Leftwich1Ray Wilson2Leonela Z. Carabajal Paladino3Sanjay Basu4Sara Rooney5Zach N. Adelman6Luke Alphey7Arthropod Genetics, The Pirbright InstituteArthropod Genetics, The Pirbright InstituteArthropod Genetics, The Pirbright InstituteArthropod Genetics, The Pirbright InstituteArthropod Genetics, The Pirbright InstituteArthropod Genetics, The Pirbright InstituteDepartment of Entomology, Texas A&M UniversityArthropod Genetics, The Pirbright InstituteAbstract Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.https://doi.org/10.1038/s41598-023-47777-3 |
| spellingShingle | Michelle A. E. Anderson Philip T. Leftwich Ray Wilson Leonela Z. Carabajal Paladino Sanjay Basu Sara Rooney Zach N. Adelman Luke Alphey AePUb promoter length modulates gene expression in Aedes aegypti Scientific Reports |
| title | AePUb promoter length modulates gene expression in Aedes aegypti |
| title_full | AePUb promoter length modulates gene expression in Aedes aegypti |
| title_fullStr | AePUb promoter length modulates gene expression in Aedes aegypti |
| title_full_unstemmed | AePUb promoter length modulates gene expression in Aedes aegypti |
| title_short | AePUb promoter length modulates gene expression in Aedes aegypti |
| title_sort | aepub promoter length modulates gene expression in aedes aegypti |
| url | https://doi.org/10.1038/s41598-023-47777-3 |
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