FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis
The precise diagnosis of bacterial meningitis is essential. Cytological and biochemical examination of cerebrospinal fluid (CSF) are not specific. Conventional methods for bacterial meningitis lack sensitivity or take too long for a final result. Therefore, other methods for rapid and accurate diagn...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S141386701930474X |
| _version_ | 1819261675104108544 |
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| author | Renan Barros Domingues Márcio Vega dos Santos Fernando Brunale Vilela de Moura Leite Carlos Senne |
| author_facet | Renan Barros Domingues Márcio Vega dos Santos Fernando Brunale Vilela de Moura Leite Carlos Senne |
| author_sort | Renan Barros Domingues |
| collection | DOAJ |
| description | The precise diagnosis of bacterial meningitis is essential. Cytological and biochemical examination of cerebrospinal fluid (CSF) are not specific. Conventional methods for bacterial meningitis lack sensitivity or take too long for a final result. Therefore, other methods for rapid and accurate diagnosis of central nervous system infections are required. FilmArray meningitis/encephalitis (ME) panel is a PCR multiplex for simultaneous and rapid identification of 14 pathogens, including 6 bacteria, 7 viruses, and Cryptococcus. We evaluated 436 CSF samples submitted to FilmArray ME Panel. Among them, 25 cases were positive for bacteria, being Streptococcus pneumonia the most frequent (48 %). Among positive cases for bacteria, 60 % were positive only with FilmArray. All the bacterial meningitis cases in which the only positive test was FilmArray had CSF findings suggestive of bacterial meningitis, including neutrophilic pleocytosis, increased CSF protein and lactate, and decreased CSF glucose. These findings suggest that FilmArray may increase the diagnostic sensitivity for bacterial meningitis. Keywords: Meningitis, FilmArray Meningitis/Encephalitis, Cerebrospinal fluid, Polymerase chain reaction |
| first_indexed | 2024-12-23T19:45:34Z |
| format | Article |
| id | doaj.art-e4bb68787c164eee84ff90f7cd04269b |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-23T19:45:34Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-e4bb68787c164eee84ff90f7cd04269b2022-12-21T17:33:33ZengElsevierBrazilian Journal of Infectious Diseases1413-86702019-11-01236468470FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitisRenan Barros Domingues0Márcio Vega dos Santos1Fernando Brunale Vilela de Moura Leite2Carlos Senne3Corresponding author.; Senne Líquor Diagnóstico, São Paulo/SP, BrazilSenne Líquor Diagnóstico, São Paulo/SP, BrazilSenne Líquor Diagnóstico, São Paulo/SP, BrazilSenne Líquor Diagnóstico, São Paulo/SP, BrazilThe precise diagnosis of bacterial meningitis is essential. Cytological and biochemical examination of cerebrospinal fluid (CSF) are not specific. Conventional methods for bacterial meningitis lack sensitivity or take too long for a final result. Therefore, other methods for rapid and accurate diagnosis of central nervous system infections are required. FilmArray meningitis/encephalitis (ME) panel is a PCR multiplex for simultaneous and rapid identification of 14 pathogens, including 6 bacteria, 7 viruses, and Cryptococcus. We evaluated 436 CSF samples submitted to FilmArray ME Panel. Among them, 25 cases were positive for bacteria, being Streptococcus pneumonia the most frequent (48 %). Among positive cases for bacteria, 60 % were positive only with FilmArray. All the bacterial meningitis cases in which the only positive test was FilmArray had CSF findings suggestive of bacterial meningitis, including neutrophilic pleocytosis, increased CSF protein and lactate, and decreased CSF glucose. These findings suggest that FilmArray may increase the diagnostic sensitivity for bacterial meningitis. Keywords: Meningitis, FilmArray Meningitis/Encephalitis, Cerebrospinal fluid, Polymerase chain reactionhttp://www.sciencedirect.com/science/article/pii/S141386701930474X |
| spellingShingle | Renan Barros Domingues Márcio Vega dos Santos Fernando Brunale Vilela de Moura Leite Carlos Senne FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis Brazilian Journal of Infectious Diseases |
| title | FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis |
| title_full | FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis |
| title_fullStr | FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis |
| title_full_unstemmed | FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis |
| title_short | FilmArray Meningitis/Encephalitis (ME) panel in the diagnosis of bacterial meningitis |
| title_sort | filmarray meningitis encephalitis me panel in the diagnosis of bacterial meningitis |
| url | http://www.sciencedirect.com/science/article/pii/S141386701930474X |
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