Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain
Abstract Background Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-024-00680-9 |
| _version_ | 1827321525347287040 |
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| author | Hui Chen Hao Xu Ruishan Liu Jian Shen Beiwen Zheng Lanjuan Li |
| author_facet | Hui Chen Hao Xu Ruishan Liu Jian Shen Beiwen Zheng Lanjuan Li |
| author_sort | Hui Chen |
| collection | DOAJ |
| description | Abstract Background Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry the bla IMP−4 and bla SFO−1 genes. This study aims to discern its antibiotic resistance phenotype and genomic characteristics. Methods Species identification was conducted using MALDI-TOF/MS. PCR identification confirmed the presence of the bla IMP−4 and bla SFO−1 genes. Antibiotic resistance phenotype and genomic characteristics were detected by antimicrobial susceptibility testing and whole-genome sequencing. Plasmid characterization was carried out through S1-PFGE, conjugation experiments, Southern blot, and comparative genomic analysis. Results K. variicola 4253 belonged to ST347, and demonstrated resistance to broad-spectrum β-lactamase drugs and tigecycline while being insensitive to imipenem and meropenem. The bla IMP−4 and bla SFO−1 genes harbored on the plasmid p4253-imp. The replicon type of p4253-imp was identified as IncHI5B, representing a multidrug-resistant plasmid capable of horizontal transfer and mediating the dissemination of drug resistance. The bla IMP−4 gene was located on the In809-like integrative element (Intl1-bla IMP−4 -aacA4-catB3), which circulates in Acinetobacter and Enterobacteriaceae. Conclusions This study reports the presence of a strain of K. variicola, which is insensitive to tigecycline, carrying a plasmid harboring bla IMP−4 and bla SFO−1. It is highly likely that the strain acquired this plasmid through horizontal transfer. The bla IMP−4 array (Intl1-bla IMP−4 -aacA4-catB3) is also mobile in Acinetobacter and Enterobacteriaceae. So it is essential to enhance clinical awareness and conduct epidemiological surveillance on multidrug-resistant K. variicola, conjugative plasmids carrying bla IMP−4 , and the In809 integrative element. |
| first_indexed | 2024-04-25T01:08:49Z |
| format | Article |
| id | doaj.art-e4bbc198744941609c62263dab1fe2db |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-04-25T01:08:49Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-e4bbc198744941609c62263dab1fe2db2024-03-10T12:07:53ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112024-03-012311810.1186/s12941-024-00680-9Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strainHui Chen0Hao Xu1Ruishan Liu2Jian Shen3Beiwen Zheng4Lanjuan Li5State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of MedicineState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of MedicineState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of MedicineState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of MedicineState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of MedicineState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of MedicineAbstract Background Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry the bla IMP−4 and bla SFO−1 genes. This study aims to discern its antibiotic resistance phenotype and genomic characteristics. Methods Species identification was conducted using MALDI-TOF/MS. PCR identification confirmed the presence of the bla IMP−4 and bla SFO−1 genes. Antibiotic resistance phenotype and genomic characteristics were detected by antimicrobial susceptibility testing and whole-genome sequencing. Plasmid characterization was carried out through S1-PFGE, conjugation experiments, Southern blot, and comparative genomic analysis. Results K. variicola 4253 belonged to ST347, and demonstrated resistance to broad-spectrum β-lactamase drugs and tigecycline while being insensitive to imipenem and meropenem. The bla IMP−4 and bla SFO−1 genes harbored on the plasmid p4253-imp. The replicon type of p4253-imp was identified as IncHI5B, representing a multidrug-resistant plasmid capable of horizontal transfer and mediating the dissemination of drug resistance. The bla IMP−4 gene was located on the In809-like integrative element (Intl1-bla IMP−4 -aacA4-catB3), which circulates in Acinetobacter and Enterobacteriaceae. Conclusions This study reports the presence of a strain of K. variicola, which is insensitive to tigecycline, carrying a plasmid harboring bla IMP−4 and bla SFO−1. It is highly likely that the strain acquired this plasmid through horizontal transfer. The bla IMP−4 array (Intl1-bla IMP−4 -aacA4-catB3) is also mobile in Acinetobacter and Enterobacteriaceae. So it is essential to enhance clinical awareness and conduct epidemiological surveillance on multidrug-resistant K. variicola, conjugative plasmids carrying bla IMP−4 , and the In809 integrative element.https://doi.org/10.1186/s12941-024-00680-9Klebsiella variicolaTigecyclineIncHI5Bbla IMP−4In809 |
| spellingShingle | Hui Chen Hao Xu Ruishan Liu Jian Shen Beiwen Zheng Lanjuan Li Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain Annals of Clinical Microbiology and Antimicrobials Klebsiella variicola Tigecycline IncHI5B bla IMP−4 In809 |
| title | Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain |
| title_full | Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain |
| title_fullStr | Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain |
| title_full_unstemmed | Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain |
| title_short | Coexistence of bla IMP−4 and bla SFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain |
| title_sort | coexistence of bla imp 4 and bla sfo 1 in an inchi5b plasmid harbored by tigecycline non susceptible klebsiella variicola strain |
| topic | Klebsiella variicola Tigecycline IncHI5B bla IMP−4 In809 |
| url | https://doi.org/10.1186/s12941-024-00680-9 |
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