The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) Yearlings
Photoperiod is associated to phenotypic plasticity of somatic growth in several teleost species, however, the molecular mechanisms underlying this phenomenon are currently unknown. The effect of a continuous lighting (LD 24:0), compared with the usual hatchery lighting (HL) regime, on the growth rat...
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MDPI AG
2021-04-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/4/328 |
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| author | Natalia S. Shulgina Maria V. Churova Svetlana A. Murzina Marina Yu. Krupnova Nina N. Nemova |
| author_facet | Natalia S. Shulgina Maria V. Churova Svetlana A. Murzina Marina Yu. Krupnova Nina N. Nemova |
| author_sort | Natalia S. Shulgina |
| collection | DOAJ |
| description | Photoperiod is associated to phenotypic plasticity of somatic growth in several teleost species, however, the molecular mechanisms underlying this phenomenon are currently unknown. The effect of a continuous lighting (LD 24:0), compared with the usual hatchery lighting (HL) regime, on the growth rate and gene expression of myogenic regulatory factors (MRFs: <i>MyoD1</i> paralogs, <i>Myf5</i>, and <i>MyoG</i>) myosin heavy chain (<i>MyHC</i>), and <i>MSTN</i> paralogs in the white muscles of hatchery-reared Atlantic salmon yearlings was evaluated over a 6-month period (May to October). The levels of gene expression were determined using real-time PCR. Continuous lighting was shown to have a positive effect on weight gain. <i>MyHC</i>, <i>MyoD1c</i>, <i>MyoD1b</i>, and <i>MSTN1a/b</i> mRNA expression was influenced by the light regime applied. In all the studied groups, a significant positive correlation was observed between the expression levels of MRFs and <i>MSTN</i> paralogs throughout the experiment. The study demonstrated seasonal patterns regarding the simultaneous expression of several MRFs. <i>MyoD1a</i>, <i>MyoG</i>, and <i>MyHC</i> mRNA expression levels were elevated in the mid-October, but <i>MyoD1b/c</i>, and <i>Myf5</i> mRNA levels decreased by the end of this month. In general, the findings showed that constant lighting affected the regulatory mechanisms of muscle growth processes in salmon. |
| first_indexed | 2024-03-10T12:27:29Z |
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| language | English |
| last_indexed | 2024-03-10T12:27:29Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj.art-e4c6f6f5859a4b328eba6ac3b2a89d5f2023-11-21T14:57:09ZengMDPI AGLife2075-17292021-04-0111432810.3390/life11040328The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) YearlingsNatalia S. Shulgina0Maria V. Churova1Svetlana A. Murzina2Marina Yu. Krupnova3Nina N. Nemova4Environmental Biochemistry Lab, Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Pushkinskaya st., 11, 185910 Petrozavodsk, RussiaEnvironmental Biochemistry Lab, Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Pushkinskaya st., 11, 185910 Petrozavodsk, RussiaEnvironmental Biochemistry Lab, Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Pushkinskaya st., 11, 185910 Petrozavodsk, RussiaEnvironmental Biochemistry Lab, Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Pushkinskaya st., 11, 185910 Petrozavodsk, RussiaEnvironmental Biochemistry Lab, Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Pushkinskaya st., 11, 185910 Petrozavodsk, RussiaPhotoperiod is associated to phenotypic plasticity of somatic growth in several teleost species, however, the molecular mechanisms underlying this phenomenon are currently unknown. The effect of a continuous lighting (LD 24:0), compared with the usual hatchery lighting (HL) regime, on the growth rate and gene expression of myogenic regulatory factors (MRFs: <i>MyoD1</i> paralogs, <i>Myf5</i>, and <i>MyoG</i>) myosin heavy chain (<i>MyHC</i>), and <i>MSTN</i> paralogs in the white muscles of hatchery-reared Atlantic salmon yearlings was evaluated over a 6-month period (May to October). The levels of gene expression were determined using real-time PCR. Continuous lighting was shown to have a positive effect on weight gain. <i>MyHC</i>, <i>MyoD1c</i>, <i>MyoD1b</i>, and <i>MSTN1a/b</i> mRNA expression was influenced by the light regime applied. In all the studied groups, a significant positive correlation was observed between the expression levels of MRFs and <i>MSTN</i> paralogs throughout the experiment. The study demonstrated seasonal patterns regarding the simultaneous expression of several MRFs. <i>MyoD1a</i>, <i>MyoG</i>, and <i>MyHC</i> mRNA expression levels were elevated in the mid-October, but <i>MyoD1b/c</i>, and <i>Myf5</i> mRNA levels decreased by the end of this month. In general, the findings showed that constant lighting affected the regulatory mechanisms of muscle growth processes in salmon.https://www.mdpi.com/2075-1729/11/4/328Atlantic salmonmuscle-specific gene expressiongrowthphotoperiod regimes |
| spellingShingle | Natalia S. Shulgina Maria V. Churova Svetlana A. Murzina Marina Yu. Krupnova Nina N. Nemova The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) Yearlings Life Atlantic salmon muscle-specific gene expression growth photoperiod regimes |
| title | The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) Yearlings |
| title_full | The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) Yearlings |
| title_fullStr | The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) Yearlings |
| title_full_unstemmed | The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) Yearlings |
| title_short | The Effect of Continuous Light on Growth and Muscle-Specific Gene Expression in Atlantic Salmon (<i>Salmo salar</i> L.) Yearlings |
| title_sort | effect of continuous light on growth and muscle specific gene expression in atlantic salmon i salmo salar i l yearlings |
| topic | Atlantic salmon muscle-specific gene expression growth photoperiod regimes |
| url | https://www.mdpi.com/2075-1729/11/4/328 |
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