Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling.
Actin polymerization plays a critical role in activated T lymphocytes both in regulating T cell receptor (TCR)-induced immunological synapse (IS) formation and signaling. Using gene targeting, we demonstrate that the hematopoietic specific, actin- and Arp2/3 complex-binding protein coronin-1A contri...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2568942?pdf=render |
| _version_ | 1819105725719248896 |
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| author | Bénédicte Mugnier Béatrice Nal Christophe Verthuy Claude Boyer David Lam Lionel Chasson Vincent Nieoullon Geneviève Chazal Xiao-Jun Guo Hai-Tao He Dominique Rueff-Juy Andrés Alcover Pierre Ferrier |
| author_facet | Bénédicte Mugnier Béatrice Nal Christophe Verthuy Claude Boyer David Lam Lionel Chasson Vincent Nieoullon Geneviève Chazal Xiao-Jun Guo Hai-Tao He Dominique Rueff-Juy Andrés Alcover Pierre Ferrier |
| author_sort | Bénédicte Mugnier |
| collection | DOAJ |
| description | Actin polymerization plays a critical role in activated T lymphocytes both in regulating T cell receptor (TCR)-induced immunological synapse (IS) formation and signaling. Using gene targeting, we demonstrate that the hematopoietic specific, actin- and Arp2/3 complex-binding protein coronin-1A contributes to both processes. Coronin-1A-deficient mice specifically showed alterations in terminal development and the survival of alpha beta T cells, together with defects in cell activation and cytokine production following TCR triggering. The mutant T cells further displayed excessive accumulation yet reduced dynamics of F-actin and the WASP-Arp2/3 machinery at the IS, correlating with extended cell-cell contact. Cell signaling was also affected with the basal activation of the stress kinases sAPK/JNK1/2; and deficits in TCR-induced Ca2+ influx and phosphorylation and degradation of the inhibitor of NF-kappaB (I kappa B). Coronin-1A therefore links cytoskeleton plasticity with the functioning of discrete TCR signaling components. This function may be required to adjust TCR responses to selecting ligands accounting in part for the homeostasis defect that impacts alpha beta T cells in coronin-1A deficient mice, with the exclusion of other lympho/hematopoietic lineages. |
| first_indexed | 2024-12-22T02:26:49Z |
| format | Article |
| id | doaj.art-e4c80882934b49339b28a5649c8e007c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T02:26:49Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e4c80882934b49339b28a5649c8e007c2022-12-21T18:41:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01310e346710.1371/journal.pone.0003467Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling.Bénédicte MugnierBéatrice NalChristophe VerthuyClaude BoyerDavid LamLionel ChassonVincent NieoullonGeneviève ChazalXiao-Jun GuoHai-Tao HeDominique Rueff-JuyAndrés AlcoverPierre FerrierActin polymerization plays a critical role in activated T lymphocytes both in regulating T cell receptor (TCR)-induced immunological synapse (IS) formation and signaling. Using gene targeting, we demonstrate that the hematopoietic specific, actin- and Arp2/3 complex-binding protein coronin-1A contributes to both processes. Coronin-1A-deficient mice specifically showed alterations in terminal development and the survival of alpha beta T cells, together with defects in cell activation and cytokine production following TCR triggering. The mutant T cells further displayed excessive accumulation yet reduced dynamics of F-actin and the WASP-Arp2/3 machinery at the IS, correlating with extended cell-cell contact. Cell signaling was also affected with the basal activation of the stress kinases sAPK/JNK1/2; and deficits in TCR-induced Ca2+ influx and phosphorylation and degradation of the inhibitor of NF-kappaB (I kappa B). Coronin-1A therefore links cytoskeleton plasticity with the functioning of discrete TCR signaling components. This function may be required to adjust TCR responses to selecting ligands accounting in part for the homeostasis defect that impacts alpha beta T cells in coronin-1A deficient mice, with the exclusion of other lympho/hematopoietic lineages.http://europepmc.org/articles/PMC2568942?pdf=render |
| spellingShingle | Bénédicte Mugnier Béatrice Nal Christophe Verthuy Claude Boyer David Lam Lionel Chasson Vincent Nieoullon Geneviève Chazal Xiao-Jun Guo Hai-Tao He Dominique Rueff-Juy Andrés Alcover Pierre Ferrier Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling. PLoS ONE |
| title | Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling. |
| title_full | Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling. |
| title_fullStr | Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling. |
| title_full_unstemmed | Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling. |
| title_short | Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling. |
| title_sort | coronin 1a links cytoskeleton dynamics to tcr alpha beta induced cell signaling |
| url | http://europepmc.org/articles/PMC2568942?pdf=render |
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