Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing
We applied multispacer typing (MST) by incorporating 9 variable intergenic spacers to Bartonella henselae DNA detected in lymph node biopsy specimens from 70 patients with cat-scratch disease (CSD), in cardiac valve specimens from 2 patients with endocarditis, and in 3 human isolates from patients w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2007-08-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/13/8/07-0085_article |
| _version_ | 1818416894390042624 |
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| author | Wenjun Li Didier Raoult Pierre-Edouard Fournier |
| author_facet | Wenjun Li Didier Raoult Pierre-Edouard Fournier |
| author_sort | Wenjun Li |
| collection | DOAJ |
| description | We applied multispacer typing (MST) by incorporating 9 variable intergenic spacers to Bartonella henselae DNA detected in lymph node biopsy specimens from 70 patients with cat-scratch disease (CSD), in cardiac valve specimens from 2 patients with endocarditis, and in 3 human isolates from patients with bacillary angiomatosis, CSD, and endocarditis. Sixteen MST genotypes were found, 5 previously identified in cats and 11 new. Of the studied DNA, 78.7% belonged to 2 genotypes, which were phylogenetically organized into 4 lineages. Human strains were mostly grouped within 2 lineages, previously identified as Marseille and Houston-1. Our results suggest a greater genetic diversity in human-infecting B. henselae than what has previously been evaluated by using other genotyping methods. However, the diversity is not significantly different from that of cat strains. MST is thus a suitable genotyping tool for evaluating the genetic heterogeneity of B. henselae among isolates obtained from human patients. |
| first_indexed | 2024-12-14T11:58:08Z |
| format | Article |
| id | doaj.art-e4c8ffa4710e4ad6b1d45f16b5d65d62 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-14T11:58:08Z |
| publishDate | 2007-08-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-e4c8ffa4710e4ad6b1d45f16b5d65d622022-12-21T23:02:04ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592007-08-011381178117810.3201/eid1308.070085Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer TypingWenjun LiDidier RaoultPierre-Edouard FournierWe applied multispacer typing (MST) by incorporating 9 variable intergenic spacers to Bartonella henselae DNA detected in lymph node biopsy specimens from 70 patients with cat-scratch disease (CSD), in cardiac valve specimens from 2 patients with endocarditis, and in 3 human isolates from patients with bacillary angiomatosis, CSD, and endocarditis. Sixteen MST genotypes were found, 5 previously identified in cats and 11 new. Of the studied DNA, 78.7% belonged to 2 genotypes, which were phylogenetically organized into 4 lineages. Human strains were mostly grouped within 2 lineages, previously identified as Marseille and Houston-1. Our results suggest a greater genetic diversity in human-infecting B. henselae than what has previously been evaluated by using other genotyping methods. However, the diversity is not significantly different from that of cat strains. MST is thus a suitable genotyping tool for evaluating the genetic heterogeneity of B. henselae among isolates obtained from human patients.https://wwwnc.cdc.gov/eid/article/13/8/07-0085_articleBartonellagenetic heterogeneitygene sequencesmultispacer typinggenotypingresearch |
| spellingShingle | Wenjun Li Didier Raoult Pierre-Edouard Fournier Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing Emerging Infectious Diseases Bartonella genetic heterogeneity gene sequences multispacer typing genotyping research |
| title | Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing |
| title_full | Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing |
| title_fullStr | Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing |
| title_full_unstemmed | Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing |
| title_short | Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing |
| title_sort | genetic diversity of bartonella henselae in human infection detected with multispacer typing |
| topic | Bartonella genetic heterogeneity gene sequences multispacer typing genotyping research |
| url | https://wwwnc.cdc.gov/eid/article/13/8/07-0085_article |
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