Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis
The emergence and development of induced pluripotent stem cells (iPSCs) provides an approach to understand the regulatory mechanisms of cell pluripotency and demonstrates the great potential of iPSCs in disease modeling. Acute myelitis defines a group of inflammatory diseases that cause acute nerve...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1172385/full |
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| author | Shuo Cao Shuo Cao Xinyue Gao Xinyue Gao Fangyuan Liu Fangyuan Liu Yanglin Chen Yanglin Chen Qin Na Qin Na Qin Na Qiaoqiao Meng Qiaoqiao Meng Peng Shao Peng Shao Chen Chen Chen Chen Yongli Song Yongli Song Baojiang Wu Baojiang Wu Xihe Li Xihe Li Xihe Li Siqin Bao Siqin Bao |
| author_facet | Shuo Cao Shuo Cao Xinyue Gao Xinyue Gao Fangyuan Liu Fangyuan Liu Yanglin Chen Yanglin Chen Qin Na Qin Na Qin Na Qiaoqiao Meng Qiaoqiao Meng Peng Shao Peng Shao Chen Chen Chen Chen Yongli Song Yongli Song Baojiang Wu Baojiang Wu Xihe Li Xihe Li Xihe Li Siqin Bao Siqin Bao |
| author_sort | Shuo Cao |
| collection | DOAJ |
| description | The emergence and development of induced pluripotent stem cells (iPSCs) provides an approach to understand the regulatory mechanisms of cell pluripotency and demonstrates the great potential of iPSCs in disease modeling. Acute myelitis defines a group of inflammatory diseases that cause acute nerve damage in the spinal cord; however, its pathophysiology remains to be elusive. In this study, we derived skin fibroblasts from a patient with acute myelitis (P-HAF) and then reprogrammed P-HAF cells to iPSCs using eight exogenous factors (namely, OCT4, SOX2, c-MYC, KLF4, NANOG, LIN28, RARG, and LRH1). We performed transcriptomic analysis of the P-HAF and compared the biological characteristics of the iPSCs derived from the patient (P-iPSCs) with those derived from normal individuals in terms of pluripotency, transcriptomic characteristics, and differentiation ability toward the ectoderm. Compared to the control iPSCs, the P-iPSCs displayed similar features of pluripotency and comparable capability of ectoderm differentiation in the specified culture. However, when tested in the common medium, the P-iPSCs showed attenuated potential for ectoderm differentiation. The transcriptomic analysis revealed that pathways enriched in P-iPSCs included those involved in Wnt signaling. To this end, we treated iPSCs and P-iPSCs with the Wnt signaling pathway inhibitor IWR1 during the differentiation process and found that the expression of the ectoderm marker Sox1 was increased significantly in P-iPSCs. This study provides a novel approach to investigating the pathogenesis of acute myelitis. |
| first_indexed | 2024-03-12T23:40:10Z |
| format | Article |
| id | doaj.art-e4c95ae980604f10b2fe005ef77cc672 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-12T23:40:10Z |
| publishDate | 2023-07-01 |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-e4c95ae980604f10b2fe005ef77cc6722023-07-14T23:00:26ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-07-011110.3389/fcell.2023.11723851172385Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitisShuo Cao0Shuo Cao1Xinyue Gao2Xinyue Gao3Fangyuan Liu4Fangyuan Liu5Yanglin Chen6Yanglin Chen7Qin Na8Qin Na9Qin Na10Qiaoqiao Meng11Qiaoqiao Meng12Peng Shao13Peng Shao14Chen Chen15Chen Chen16Yongli Song17Yongli Song18Baojiang Wu19Baojiang Wu20Xihe Li21Xihe Li22Xihe Li23Siqin Bao24Siqin Bao25The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaCollege of Basic Medicine, Inner Mongolia Medical University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaInner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, ChinaResearch Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Hohhot, ChinaThe emergence and development of induced pluripotent stem cells (iPSCs) provides an approach to understand the regulatory mechanisms of cell pluripotency and demonstrates the great potential of iPSCs in disease modeling. Acute myelitis defines a group of inflammatory diseases that cause acute nerve damage in the spinal cord; however, its pathophysiology remains to be elusive. In this study, we derived skin fibroblasts from a patient with acute myelitis (P-HAF) and then reprogrammed P-HAF cells to iPSCs using eight exogenous factors (namely, OCT4, SOX2, c-MYC, KLF4, NANOG, LIN28, RARG, and LRH1). We performed transcriptomic analysis of the P-HAF and compared the biological characteristics of the iPSCs derived from the patient (P-iPSCs) with those derived from normal individuals in terms of pluripotency, transcriptomic characteristics, and differentiation ability toward the ectoderm. Compared to the control iPSCs, the P-iPSCs displayed similar features of pluripotency and comparable capability of ectoderm differentiation in the specified culture. However, when tested in the common medium, the P-iPSCs showed attenuated potential for ectoderm differentiation. The transcriptomic analysis revealed that pathways enriched in P-iPSCs included those involved in Wnt signaling. To this end, we treated iPSCs and P-iPSCs with the Wnt signaling pathway inhibitor IWR1 during the differentiation process and found that the expression of the ectoderm marker Sox1 was increased significantly in P-iPSCs. This study provides a novel approach to investigating the pathogenesis of acute myelitis.https://www.frontiersin.org/articles/10.3389/fcell.2023.1172385/fullinduced pluripotent stem cellsacute myelitisreprogrammingectodermdifferentiation |
| spellingShingle | Shuo Cao Shuo Cao Xinyue Gao Xinyue Gao Fangyuan Liu Fangyuan Liu Yanglin Chen Yanglin Chen Qin Na Qin Na Qin Na Qiaoqiao Meng Qiaoqiao Meng Peng Shao Peng Shao Chen Chen Chen Chen Yongli Song Yongli Song Baojiang Wu Baojiang Wu Xihe Li Xihe Li Xihe Li Siqin Bao Siqin Bao Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis Frontiers in Cell and Developmental Biology induced pluripotent stem cells acute myelitis reprogramming ectoderm differentiation |
| title | Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis |
| title_full | Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis |
| title_fullStr | Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis |
| title_full_unstemmed | Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis |
| title_short | Derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis |
| title_sort | derivation and characteristics of induced pluripotent stem cells from a patient with acute myelitis |
| topic | induced pluripotent stem cells acute myelitis reprogramming ectoderm differentiation |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1172385/full |
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