Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay
Escherichia coli O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a Ci...
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MDPI AG
2017-04-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/17/4/753 |
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| author | Song Cheng Ming-hui Chen Gang-gang Zhang Zhi-biao Yu Dao-feng Liu Yong-hua Xiong Hua Wei Wei-hua Lai |
| author_facet | Song Cheng Ming-hui Chen Gang-gang Zhang Zhi-biao Yu Dao-feng Liu Yong-hua Xiong Hua Wei Wei-hua Lai |
| author_sort | Song Cheng |
| collection | DOAJ |
| description | Escherichia coli O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a Citrobacter freundii strain was isolated from the ground pork samples and identified by using CHROmagarTM plates, API 20E, and 16S RNA sequencing. Since C. freundii showed cross-reactivity with E. coli O157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient detection of E. coli O157:H7 in ground pork was developed in this study to minimize these false positives. MacConkey broth was optimized for E. coli O157:H7 enrichment and C. freundii inhibition by the addition of 5 mg/L potassium tellurite and 0.10 mg/L cefixime. Using the proposed modified enrichment procedure, the false-positive rate of ground pork samples spiked with 100 CFU/g C. freundii decreased to 5%. |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T08:30:39Z |
| publishDate | 2017-04-01 |
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| spelling | doaj.art-e4d13e656d2e402aa107cfc3cc9a4e232022-12-22T02:54:16ZengMDPI AGSensors1424-82202017-04-0117475310.3390/s17040753s17040753Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow ImmunoassaySong Cheng0Ming-hui Chen1Gang-gang Zhang2Zhi-biao Yu3Dao-feng Liu4Yong-hua Xiong5Hua Wei6Wei-hua Lai7State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaDepartment of Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaEscherichia coli O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a Citrobacter freundii strain was isolated from the ground pork samples and identified by using CHROmagarTM plates, API 20E, and 16S RNA sequencing. Since C. freundii showed cross-reactivity with E. coli O157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient detection of E. coli O157:H7 in ground pork was developed in this study to minimize these false positives. MacConkey broth was optimized for E. coli O157:H7 enrichment and C. freundii inhibition by the addition of 5 mg/L potassium tellurite and 0.10 mg/L cefixime. Using the proposed modified enrichment procedure, the false-positive rate of ground pork samples spiked with 100 CFU/g C. freundii decreased to 5%.http://www.mdpi.com/1424-8220/17/4/753enrichmentE. coli O157:H7ground pork samplefalse positive |
| spellingShingle | Song Cheng Ming-hui Chen Gang-gang Zhang Zhi-biao Yu Dao-feng Liu Yong-hua Xiong Hua Wei Wei-hua Lai Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay Sensors enrichment E. coli O157:H7 ground pork sample false positive |
| title | Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay |
| title_full | Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay |
| title_fullStr | Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay |
| title_full_unstemmed | Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay |
| title_short | Strategy for Accurate Detection of Escherichia Coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay |
| title_sort | strategy for accurate detection of escherichia coli o157 h7 in ground pork using a lateral flow immunoassay |
| topic | enrichment E. coli O157:H7 ground pork sample false positive |
| url | http://www.mdpi.com/1424-8220/17/4/753 |
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