IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREES

Melina (Gmelina arborea Roxb.) is a forest species with fast growing, tall trees. Melina propagation is based on cuttings isolated from juvenile seedlings. The present research focused on micropropagation of melina plants, grown from cuttings isolated from mature trees. Explants were surface disinfe...

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Main Authors: Isidro E. Suárez, Claudia C. Acosta, Kellen C. Gatti
Format: Article
Language:Spanish
Published: Universidad de Ciencias Aplicadas y Ambientales 2013-06-01
Series:Revista U.D.C.A Actualidad & Divulgación Científica
Subjects:
Online Access:http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0123-42262013000100012&lng=en&tlng=en
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author Isidro E. Suárez
Claudia C. Acosta
Kellen C. Gatti
author_facet Isidro E. Suárez
Claudia C. Acosta
Kellen C. Gatti
author_sort Isidro E. Suárez
collection DOAJ
description Melina (Gmelina arborea Roxb.) is a forest species with fast growing, tall trees. Melina propagation is based on cuttings isolated from juvenile seedlings. The present research focused on micropropagation of melina plants, grown from cuttings isolated from mature trees. Explants were surface disinfected with NaOCl (0.5%) and established on semisolid MS with (in mg L-1) myo-inositol (100), sucrose (30.000) and TC-agar (7.000). The effect of BAP (0; 0.44; 1.33 and 2.22μM) on shoot proliferation, AIB (0, 0.49, 1.48 and 2.46μM) on in vitro rooting and substrate (sand and peat) on ex vitro survival were evaluated. Treatments were distributed with a complete randomized design, 20 replicates per treatment and 80 total experimental units were evaluated. Collected data showed that compared to control BAP increased shoot proliferation, shoot length and leaf number, while IBA induced higher root number, root length and rooting percentages. Higher survival rates were observed when plants were transferred to peat.
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spelling doaj.art-e4d7338704864848b7e153acfb7581d82022-12-21T19:03:02ZspaUniversidad de Ciencias Aplicadas y AmbientalesRevista U.D.C.A Actualidad & Divulgación Científica0123-42262013-06-0116197102S0123-42262013000100012IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREESIsidro E. Suárez0Claudia C. Acosta1Kellen C. Gatti2Universidad de CórdobaUniversidad de CórdobaUniversidad de CórdobaMelina (Gmelina arborea Roxb.) is a forest species with fast growing, tall trees. Melina propagation is based on cuttings isolated from juvenile seedlings. The present research focused on micropropagation of melina plants, grown from cuttings isolated from mature trees. Explants were surface disinfected with NaOCl (0.5%) and established on semisolid MS with (in mg L-1) myo-inositol (100), sucrose (30.000) and TC-agar (7.000). The effect of BAP (0; 0.44; 1.33 and 2.22μM) on shoot proliferation, AIB (0, 0.49, 1.48 and 2.46μM) on in vitro rooting and substrate (sand and peat) on ex vitro survival were evaluated. Treatments were distributed with a complete randomized design, 20 replicates per treatment and 80 total experimental units were evaluated. Collected data showed that compared to control BAP increased shoot proliferation, shoot length and leaf number, while IBA induced higher root number, root length and rooting percentages. Higher survival rates were observed when plants were transferred to peat.http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0123-42262013000100012&lng=en&tlng=enBAPenraizamiento in vitroforestalmicropropagación
spellingShingle Isidro E. Suárez
Claudia C. Acosta
Kellen C. Gatti
IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREES
Revista U.D.C.A Actualidad & Divulgación Científica
BAP
enraizamiento in vitro
forestal
micropropagación
title IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREES
title_full IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREES
title_fullStr IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREES
title_full_unstemmed IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREES
title_short IN VITRO MULTIPLICATION OF Gmelina arborea Roxb. ADULT TREES
title_sort in vitro multiplication of gmelina arborea roxb adult trees
topic BAP
enraizamiento in vitro
forestal
micropropagación
url http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0123-42262013000100012&lng=en&tlng=en
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