Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols
In this study, a laccase LAC-Yang1 was successfully purified from a white-rot fungus strain <i>Pleurotus ostreatus</i> strain yang1 with high laccase activity. The enzymatic properties of LAC-Yang1 and its ability to degrade and detoxify chlorophenols such as 2,6-dichlorophenol and 2,3,6...
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/2/473 |
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| author | Xinping Liu Wei Deng Yang Yang |
| author_facet | Xinping Liu Wei Deng Yang Yang |
| author_sort | Xinping Liu |
| collection | DOAJ |
| description | In this study, a laccase LAC-Yang1 was successfully purified from a white-rot fungus strain <i>Pleurotus ostreatus</i> strain yang1 with high laccase activity. The enzymatic properties of LAC-Yang1 and its ability to degrade and detoxify chlorophenols such as 2,6-dichlorophenol and 2,3,6-trichlorophenol were systematically studied. LAC-Yang1 showed a strong tolerance to extremely acidic conditions and strong stability under strong alkaline conditions (pH 9–12). LAC-Yang1 also exhibited a strong tolerance to different inhibitors (EDTA, SDS), metal ions (Mn<sup>2+</sup>, Cu<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup>, K<sup>+</sup>, Zn<sup>2+</sup>, Al<sup>3+</sup>, Co<sup>2+</sup>, and metal ion mixtures), and organic solvents (glycerol, propylene glycol). LAC-Yang1 showed good stability in the presence of Mg<sup>2+</sup>, Mn<sup>2+</sup>, glycerol, and ethylene glycol. Our results reveal the strong degradation ability of this laccase for high concentrations of chlorophenols (especially 2,6-dichlorophenol) and chlorophenol mixtures (2,6-dichlorophenol + 2,3,6-trichlorophenol). LAC-Yang1 displayed a strong tolerance toward a variety of metal ions (Na<sup>2+</sup>, Zn<sup>2+</sup>, Mn<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup> and metal ion mixtures) and organic solvents (glycerol, ethylene glycol) in its degradation of 2,6-dichlorophenol and 2,3,6-trichlorophenol. The phytotoxicity of 2,6-dichlorophenol treated by LAC-Yang1 was significantly reduced or eliminated. LAC-Yang1 demonstrated a good detoxification effect on 2,6-dichlorophenol while degrading this compound. In conclusion, LAC-Yang1 purified from <i>Pleurotus ostreatus</i> has great application value and potential in environmental biotechnology, especially the efficient degradation and detoxification of chlorophenols. |
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| language | English |
| last_indexed | 2024-03-09T04:29:15Z |
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| spelling | doaj.art-e4de35daae6945ca8b22f6d481c8784e2023-12-03T13:38:16ZengMDPI AGMolecules1420-30492021-01-0126247310.3390/molecules26020473Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify ChlorophenolsXinping Liu0Wei Deng1Yang Yang2Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaHubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaHubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaIn this study, a laccase LAC-Yang1 was successfully purified from a white-rot fungus strain <i>Pleurotus ostreatus</i> strain yang1 with high laccase activity. The enzymatic properties of LAC-Yang1 and its ability to degrade and detoxify chlorophenols such as 2,6-dichlorophenol and 2,3,6-trichlorophenol were systematically studied. LAC-Yang1 showed a strong tolerance to extremely acidic conditions and strong stability under strong alkaline conditions (pH 9–12). LAC-Yang1 also exhibited a strong tolerance to different inhibitors (EDTA, SDS), metal ions (Mn<sup>2+</sup>, Cu<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup>, K<sup>+</sup>, Zn<sup>2+</sup>, Al<sup>3+</sup>, Co<sup>2+</sup>, and metal ion mixtures), and organic solvents (glycerol, propylene glycol). LAC-Yang1 showed good stability in the presence of Mg<sup>2+</sup>, Mn<sup>2+</sup>, glycerol, and ethylene glycol. Our results reveal the strong degradation ability of this laccase for high concentrations of chlorophenols (especially 2,6-dichlorophenol) and chlorophenol mixtures (2,6-dichlorophenol + 2,3,6-trichlorophenol). LAC-Yang1 displayed a strong tolerance toward a variety of metal ions (Na<sup>2+</sup>, Zn<sup>2+</sup>, Mn<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup> and metal ion mixtures) and organic solvents (glycerol, ethylene glycol) in its degradation of 2,6-dichlorophenol and 2,3,6-trichlorophenol. The phytotoxicity of 2,6-dichlorophenol treated by LAC-Yang1 was significantly reduced or eliminated. LAC-Yang1 demonstrated a good detoxification effect on 2,6-dichlorophenol while degrading this compound. In conclusion, LAC-Yang1 purified from <i>Pleurotus ostreatus</i> has great application value and potential in environmental biotechnology, especially the efficient degradation and detoxification of chlorophenols.https://www.mdpi.com/1420-3049/26/2/473fungilaccaseenzymatic propertieschlorophenoldegradationdetoxification |
| spellingShingle | Xinping Liu Wei Deng Yang Yang Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols Molecules fungi laccase enzymatic properties chlorophenol degradation detoxification |
| title | Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols |
| title_full | Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols |
| title_fullStr | Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols |
| title_full_unstemmed | Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols |
| title_short | Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus <i>Pleurotus ostreatus</i> Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols |
| title_sort | characterization of a novel laccase lac yang1 from white rot fungus i pleurotus ostreatus i strain yang1 with a strong ability to degrade and detoxify chlorophenols |
| topic | fungi laccase enzymatic properties chlorophenol degradation detoxification |
| url | https://www.mdpi.com/1420-3049/26/2/473 |
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