Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines
Introduction. Flaviviruses, possessing natural neurotropicity could be used in glioblastoma therapy using attenuated strains or as a delivery system for antitumor agents in an inactivated form. Objective. To investigate the sensitivity of glioblastoma and pancreatic carcinoma cell lines to vaccin...
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| Format: | Article |
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Central Research Institute for Epidemiology
2023-12-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/16100/850 |
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| author | Alina S. Nazarenko Yulia K. Biryukova Ekaterina O. Orlova Kirill N. Trachuk Alla L. Ivanova Alla V. Belyakova Nikolai B. Pestov Mikhail F. Vorovitch Aydar A. Ishmukhametov Nadezhda M. Kolyasnikova |
| author_facet | Alina S. Nazarenko Yulia K. Biryukova Ekaterina O. Orlova Kirill N. Trachuk Alla L. Ivanova Alla V. Belyakova Nikolai B. Pestov Mikhail F. Vorovitch Aydar A. Ishmukhametov Nadezhda M. Kolyasnikova |
| author_sort | Alina S. Nazarenko |
| collection | DOAJ |
| description | Introduction. Flaviviruses, possessing natural neurotropicity could be used in glioblastoma therapy using attenuated strains or as a delivery system for antitumor agents in an inactivated form.
Objective. To investigate the sensitivity of glioblastoma and pancreatic carcinoma cell lines to vaccine strains of yellow fever and tick-borne encephalitis viruses.
Materials and methods. Cell lines: glioblastoma GL-6, T98G, LN-229, pancreatic carcinoma MIA RaCa-2 and human pancreatic ductal carcinoma PANC-1. Viral strains: 17D yellow fever virus (YF), Sofjin tick-borne encephalitis virus (TBEV). Virus concentration were determined by plaque assay and quantitative PCR. Determination of cell sensitivity to viruses by MTT assay.
Results. 17D YF was effective only against pancreatic carcinoma tumor cells MIA Paca-2 and had a limited effect against PANC-1. In glioblastoma cell lines (LN229, GL6, T98G), virus had no oncolytic effect and the viral RNA concentration fell in the culture medium. Sofjin TBEV showed CPE50 against MIA Paca-2 and a very limited cytotoxic effect against PANC-1. However, it had no oncolytic effect against glioblastoma cell lines (LN229, T98G and GL6), although virus reproduction continued in these cultures. For the GL6 glioblastoma cell line, the viral RNA concentration at the level with the infection dose was determined within 13 days, despite medium replacement, while in the case of the LN229 cell line, the virus concentration increased from 1 × 109 to 1 × 1010 copies/ml.
Conclusion. Tumor behavior in organism is more complex and is determined by different microenvironmental factors and immune status. In the future, it is advisable to continue studying the antitumor oncolytic and immunomodulatory effects of viral strains 17D YF and Sofjin TBEV using in vivo models. |
| first_indexed | 2024-03-08T18:37:40Z |
| format | Article |
| id | doaj.art-e4def4e6cbf1471bbc5241c08ec63f0d |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-08T18:37:40Z |
| publishDate | 2023-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-e4def4e6cbf1471bbc5241c08ec63f0d2023-12-29T10:39:14ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972023-12-0168653654810.36233/0507-4088-2041200Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell linesAlina S. Nazarenko0https://orcid.org/0000-0002-7322-8730Yulia K. Biryukova1https://orcid.org/0000-0002-5804-4001Ekaterina O. Orlova2https://orcid.org/0009-0009-4175-0493Kirill N. Trachuk3https://orcid.org/0000-0002-2061-0274Alla L. Ivanova4https://orcid.org/0009-0002-3086-0581Alla V. Belyakova5https://orcid.org/0000-0003-4363-6394Nikolai B. Pestov6https://orcid.org/0000-0002-9973-0120Mikhail F. Vorovitch7https://orcid.org/0000-0002-7367-6357Aydar A. Ishmukhametov8https://orcid.org/0000-0001-6130-4145Nadezhda M. Kolyasnikova9https://orcid.org/0000-0002-9934-2582Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis)Introduction. Flaviviruses, possessing natural neurotropicity could be used in glioblastoma therapy using attenuated strains or as a delivery system for antitumor agents in an inactivated form. Objective. To investigate the sensitivity of glioblastoma and pancreatic carcinoma cell lines to vaccine strains of yellow fever and tick-borne encephalitis viruses. Materials and methods. Cell lines: glioblastoma GL-6, T98G, LN-229, pancreatic carcinoma MIA RaCa-2 and human pancreatic ductal carcinoma PANC-1. Viral strains: 17D yellow fever virus (YF), Sofjin tick-borne encephalitis virus (TBEV). Virus concentration were determined by plaque assay and quantitative PCR. Determination of cell sensitivity to viruses by MTT assay. Results. 17D YF was effective only against pancreatic carcinoma tumor cells MIA Paca-2 and had a limited effect against PANC-1. In glioblastoma cell lines (LN229, GL6, T98G), virus had no oncolytic effect and the viral RNA concentration fell in the culture medium. Sofjin TBEV showed CPE50 against MIA Paca-2 and a very limited cytotoxic effect against PANC-1. However, it had no oncolytic effect against glioblastoma cell lines (LN229, T98G and GL6), although virus reproduction continued in these cultures. For the GL6 glioblastoma cell line, the viral RNA concentration at the level with the infection dose was determined within 13 days, despite medium replacement, while in the case of the LN229 cell line, the virus concentration increased from 1 × 109 to 1 × 1010 copies/ml. Conclusion. Tumor behavior in organism is more complex and is determined by different microenvironmental factors and immune status. In the future, it is advisable to continue studying the antitumor oncolytic and immunomodulatory effects of viral strains 17D YF and Sofjin TBEV using in vivo models.https://virusjour.crie.ru/jour/article/viewFile/16100/850oncolytic virusesflavivirusesyellow fever virustick-borne encephalitis virusglioblastoma cellpancreatic carcinoma cellvirus sensitivityviral oncolysis |
| spellingShingle | Alina S. Nazarenko Yulia K. Biryukova Ekaterina O. Orlova Kirill N. Trachuk Alla L. Ivanova Alla V. Belyakova Nikolai B. Pestov Mikhail F. Vorovitch Aydar A. Ishmukhametov Nadezhda M. Kolyasnikova Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines Вопросы вирусологии oncolytic viruses flaviviruses yellow fever virus tick-borne encephalitis virus glioblastoma cell pancreatic carcinoma cell virus sensitivity viral oncolysis |
| title | Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines |
| title_full | Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines |
| title_fullStr | Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines |
| title_full_unstemmed | Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines |
| title_short | Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines |
| title_sort | investigation of oncolytic potential of vaccine strains of yellow fever and tick borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines |
| topic | oncolytic viruses flaviviruses yellow fever virus tick-borne encephalitis virus glioblastoma cell pancreatic carcinoma cell virus sensitivity viral oncolysis |
| url | https://virusjour.crie.ru/jour/article/viewFile/16100/850 |
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