Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti
Abstract Background Gut symbiotic microbiota plays a critical role in nutrient supply, digestion, and absorption. The bamboo snout beetle, Cyrtotrachelus buqueti, a common pest of several bamboo species, exhibits high lignocellulolytic enzyme activity and contains various CAZyme genes. However, to d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-04-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1411-1 |
| _version_ | 1811245749738930176 |
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| author | Chaobing Luo Yuanqiu Li Ying Chen Chun Fu Wencong Long Ximeng Xiao Hong Liao Yaojun Yang |
| author_facet | Chaobing Luo Yuanqiu Li Ying Chen Chun Fu Wencong Long Ximeng Xiao Hong Liao Yaojun Yang |
| author_sort | Chaobing Luo |
| collection | DOAJ |
| description | Abstract Background Gut symbiotic microbiota plays a critical role in nutrient supply, digestion, and absorption. The bamboo snout beetle, Cyrtotrachelus buqueti, a common pest of several bamboo species, exhibits high lignocellulolytic enzyme activity and contains various CAZyme genes. However, to date, no studies have evaluated the role of gut symbiotic microbiota of the snout beetle on bamboo lignocellulose degradation. Therefore, the present study investigated the role of gut symbiotic microbiota of C. buqueti on bamboo lignocellulose degradation. Results Gut symbiotic microbiota of female (CCJ), male (XCJ), and larvae (YCJ) beetles was used to treat bamboo shoot particles (BSPs) in vitro for 6 days. Scanning electron microscopy (SEM) revealed significant destruction of the lignocellulose structure after treatment, which was consistent with the degradation efficiencies of CCJ, XCJ, and YCJ for cellulose (21.11%, 17.58% and 18.74%, respectively); hemicellulose (22.22%, 27.18% and 34.20%, respectively); and lignin (19.83%, 24.30% and 32.97%, respectively). Gut symbiotic microbiota of adult and larvae beetles was then identified using 16sRNA sequencing, which revealed that four microbes: Lactococcus, Serratia, Dysgonomonas and Enterococcus, comprise approximately 84% to 94% of the microbiota. Moreover, the genomes of 45 Lactococcus, 72 Serratia, 86 Enterococcus and 4 Dysgonomonas microbes were used to analyse resident CAZyme genes. These results indicated that gut symbiotic microbiota of adult and larvae C. buqueti is involved in the lignocellulose degradation traits shown by the host. Conclusions This study shows that the gut symbiotic microbiota of C. buqueti participates in bamboo lignocellulose degradation, providing innovative findings for bamboo lignocellulose bioconversion. Furthermore, the results of this study will allow us to further isolate lignocellulose-degrading microbiota for use in bamboo lignocellulose bioconversion. |
| first_indexed | 2024-04-12T14:43:52Z |
| format | Article |
| id | doaj.art-e4dfd7a3f5a14373a2d8b30bd8d41c6e |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T14:43:52Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-e4dfd7a3f5a14373a2d8b30bd8d41c6e2022-12-22T03:28:44ZengBMCBiotechnology for Biofuels1754-68342019-04-0112111610.1186/s13068-019-1411-1Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buquetiChaobing Luo0Yuanqiu Li1Ying Chen2Chun Fu3Wencong Long4Ximeng Xiao5Hong Liao6Yaojun Yang7Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityBamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityBamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityBamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityBamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityBamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityBamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityBamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal UniversityAbstract Background Gut symbiotic microbiota plays a critical role in nutrient supply, digestion, and absorption. The bamboo snout beetle, Cyrtotrachelus buqueti, a common pest of several bamboo species, exhibits high lignocellulolytic enzyme activity and contains various CAZyme genes. However, to date, no studies have evaluated the role of gut symbiotic microbiota of the snout beetle on bamboo lignocellulose degradation. Therefore, the present study investigated the role of gut symbiotic microbiota of C. buqueti on bamboo lignocellulose degradation. Results Gut symbiotic microbiota of female (CCJ), male (XCJ), and larvae (YCJ) beetles was used to treat bamboo shoot particles (BSPs) in vitro for 6 days. Scanning electron microscopy (SEM) revealed significant destruction of the lignocellulose structure after treatment, which was consistent with the degradation efficiencies of CCJ, XCJ, and YCJ for cellulose (21.11%, 17.58% and 18.74%, respectively); hemicellulose (22.22%, 27.18% and 34.20%, respectively); and lignin (19.83%, 24.30% and 32.97%, respectively). Gut symbiotic microbiota of adult and larvae beetles was then identified using 16sRNA sequencing, which revealed that four microbes: Lactococcus, Serratia, Dysgonomonas and Enterococcus, comprise approximately 84% to 94% of the microbiota. Moreover, the genomes of 45 Lactococcus, 72 Serratia, 86 Enterococcus and 4 Dysgonomonas microbes were used to analyse resident CAZyme genes. These results indicated that gut symbiotic microbiota of adult and larvae C. buqueti is involved in the lignocellulose degradation traits shown by the host. Conclusions This study shows that the gut symbiotic microbiota of C. buqueti participates in bamboo lignocellulose degradation, providing innovative findings for bamboo lignocellulose bioconversion. Furthermore, the results of this study will allow us to further isolate lignocellulose-degrading microbiota for use in bamboo lignocellulose bioconversion.http://link.springer.com/article/10.1186/s13068-019-1411-1Lignocellulose degradationBambooGut symbiotic microbiotaCyrtotrachelus buqueti16sRNA-sequencing |
| spellingShingle | Chaobing Luo Yuanqiu Li Ying Chen Chun Fu Wencong Long Ximeng Xiao Hong Liao Yaojun Yang Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti Biotechnology for Biofuels Lignocellulose degradation Bamboo Gut symbiotic microbiota Cyrtotrachelus buqueti 16sRNA-sequencing |
| title | Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti |
| title_full | Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti |
| title_fullStr | Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti |
| title_full_unstemmed | Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti |
| title_short | Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle Cyrtotrachelus buqueti |
| title_sort | bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle cyrtotrachelus buqueti |
| topic | Lignocellulose degradation Bamboo Gut symbiotic microbiota Cyrtotrachelus buqueti 16sRNA-sequencing |
| url | http://link.springer.com/article/10.1186/s13068-019-1411-1 |
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