Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway
Bioactive peptides are relevant nutritional factors that exhibit many functions including antioxidant, antihypertensive, anticancer and antimicrobial properties. In this paper, four synthetic peptides ARHPHPHLSFM (A-11-M), AVPYPQR (A-7-R), NPYVPR (N-6-R) and KVLPVPEK (K-8-K) with sequences present i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464619306206 |
| _version_ | 1818728235708448768 |
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| author | Federica Tonolo Alessandra Folda Luca Cesaro Valeria Scalcon Oriano Marin Stefania Ferro Alberto Bindoli Maria Pia Rigobello |
| author_facet | Federica Tonolo Alessandra Folda Luca Cesaro Valeria Scalcon Oriano Marin Stefania Ferro Alberto Bindoli Maria Pia Rigobello |
| author_sort | Federica Tonolo |
| collection | DOAJ |
| description | Bioactive peptides are relevant nutritional factors that exhibit many functions including antioxidant, antihypertensive, anticancer and antimicrobial properties. In this paper, four synthetic peptides ARHPHPHLSFM (A-11-M), AVPYPQR (A-7-R), NPYVPR (N-6-R) and KVLPVPEK (K-8-K) with sequences present in milk proteins were examined for their antioxidant properties. The compounds show moderate free radical scavenging activity in the ABTS and crocin assays (A-7-R and N-6-R) and lipid peroxidation inhibition in Caco-2 cells (N-6-R and K-8-K). All peptides, in particular K-8-K, activate the Keap1-Nrf2 system by allowing the translocation of the transcription factor Nrf2 from the cytosol to nucleus. This activation triggers the overexpression of the antioxidant enzymes Trx1, TrxR1, GR, NQO1 and SOD1. Furthermore, molecular modeling shows that K-8-K is able to hinder the interaction of Nrf2 with Keap1. The reported results show that the antioxidant action in cells of these bioactive peptides is mostly due to the activation of Keap1-Nrf2 signaling pathway. |
| first_indexed | 2024-12-17T22:26:46Z |
| format | Article |
| id | doaj.art-e4e00acc2f0a4334833d1dd749678596 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T22:26:46Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e4e00acc2f0a4334833d1dd7496785962022-12-21T21:30:19ZengElsevierJournal of Functional Foods1756-46462020-01-0164103696Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathwayFederica Tonolo0Alessandra Folda1Luca Cesaro2Valeria Scalcon3Oriano Marin4Stefania Ferro5Alberto Bindoli6Maria Pia Rigobello7University of Padova, Department of Biomedical Sciences, via Ugo Bassi 58/b, 35131 Padova, ItalyUniversity of Padova, Department of Biomedical Sciences, via Ugo Bassi 58/b, 35131 Padova, ItalyUniversity of Padova, Department of Biomedical Sciences, via Ugo Bassi 58/b, 35131 Padova, ItalyUniversity of Padova, Department of Biomedical Sciences, via Ugo Bassi 58/b, 35131 Padova, ItalyUniversity of Padova, Department of Biomedical Sciences, via Ugo Bassi 58/b, 35131 Padova, ItalyUniversity of Padova, Department of Biomedical Sciences, via Ugo Bassi 58/b, 35131 Padova, ItalyInstitute of Neuroscience, CNR, viale G. Colombo 3, 35131 Padova, ItalyUniversity of Padova, Department of Biomedical Sciences, via Ugo Bassi 58/b, 35131 Padova, Italy; Corresponding author.Bioactive peptides are relevant nutritional factors that exhibit many functions including antioxidant, antihypertensive, anticancer and antimicrobial properties. In this paper, four synthetic peptides ARHPHPHLSFM (A-11-M), AVPYPQR (A-7-R), NPYVPR (N-6-R) and KVLPVPEK (K-8-K) with sequences present in milk proteins were examined for their antioxidant properties. The compounds show moderate free radical scavenging activity in the ABTS and crocin assays (A-7-R and N-6-R) and lipid peroxidation inhibition in Caco-2 cells (N-6-R and K-8-K). All peptides, in particular K-8-K, activate the Keap1-Nrf2 system by allowing the translocation of the transcription factor Nrf2 from the cytosol to nucleus. This activation triggers the overexpression of the antioxidant enzymes Trx1, TrxR1, GR, NQO1 and SOD1. Furthermore, molecular modeling shows that K-8-K is able to hinder the interaction of Nrf2 with Keap1. The reported results show that the antioxidant action in cells of these bioactive peptides is mostly due to the activation of Keap1-Nrf2 signaling pathway.http://www.sciencedirect.com/science/article/pii/S1756464619306206Bioactive peptidesAntioxidantsOxidative stressNrf2 |
| spellingShingle | Federica Tonolo Alessandra Folda Luca Cesaro Valeria Scalcon Oriano Marin Stefania Ferro Alberto Bindoli Maria Pia Rigobello Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway Journal of Functional Foods Bioactive peptides Antioxidants Oxidative stress Nrf2 |
| title | Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway |
| title_full | Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway |
| title_fullStr | Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway |
| title_full_unstemmed | Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway |
| title_short | Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway |
| title_sort | milk derived bioactive peptides exhibit antioxidant activity through the keap1 nrf2 signaling pathway |
| topic | Bioactive peptides Antioxidants Oxidative stress Nrf2 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464619306206 |
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