Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial Community
In this study, the effect of fermentation with Bacillus subtilis B-2, a salt-tolerant strain capable of producing high yield of protease, on the microbial community and the contents of biogenic amines and amino acid nitrogen (AAN) during the fermentation of low-salt fish sauce. The results of high-t...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2023-09-01
|
| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-18-008.pdf |
| _version_ | 1797652561463869440 |
|---|---|
| author | LI Yan, LI Wenjing, LI Chunsheng, YANG Daqiao, WANG Yueqi, WANG Di, CHEN Shengjun, WU Yanyan, LI Laihao |
| author_facet | LI Yan, LI Wenjing, LI Chunsheng, YANG Daqiao, WANG Yueqi, WANG Di, CHEN Shengjun, WU Yanyan, LI Laihao |
| author_sort | LI Yan, LI Wenjing, LI Chunsheng, YANG Daqiao, WANG Yueqi, WANG Di, CHEN Shengjun, WU Yanyan, LI Laihao |
| collection | DOAJ |
| description | In this study, the effect of fermentation with Bacillus subtilis B-2, a salt-tolerant strain capable of producing high yield of protease, on the microbial community and the contents of biogenic amines and amino acid nitrogen (AAN) during the fermentation of low-salt fish sauce. The results of high-throughput 16S rRNA gene sequencing showed that fermentation with B-2 reduced the richness and evenness of the microbial community, Bacillus was dominant throughout the fermentation process and the abundance of spoilage microorganisms significantly decreased. B-2 significantly inhibited the formation of histamine, putrescine, cadaverine, and tyramine, and their contents at the end of fermentation decreased by 25.9%, 35.6%, 23.6% and 9.3%, respectively, compared with those in naturally fermented fish sauce. The content of AAN in low-salt fish sauce was significantly improved by inoculated fermentation, reaching 1.23 g/100 mL after 15 days of fermentation, which was significantly higher than that of naturally fermented fish sauce (0.79 g/100 mL). The correlation network map showed that the decrease in the abundance of Brevibacterium, Dietzia, Paracoccus, Aequorivita, and Brachybacterium was the major reason for the decrease in microbial diversity at the late stage of fermentation of naturally fermented fish sauce. The abundance of Stenotrophomonas showed a significantly positive correlation with the contents of many biogenic amines in naturally fermented and B-2 fermented fish sauce, suggesting its important role in the formation of biogenic amines in low-salt fish sauce. Comparative analysis of the microbial community and quality attributes at the end of fermentation showed that the metabolism of B-2 was the main reason for the decrease in the species and abundance of spoilage microorganisms, the increase in AAN content, and the decrease in the contents of key biogenic amines. B. subtilis B-2 is expected to be developed as a special fermentation starter for fish sauce to improve the quality and safety of rapidly fermented low-salt fish sauce. |
| first_indexed | 2024-03-11T16:30:38Z |
| format | Article |
| id | doaj.art-e4e0a3597423487798ca2059f8f3424e |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-11T16:30:38Z |
| publishDate | 2023-09-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-e4e0a3597423487798ca2059f8f3424e2023-10-24T07:34:30ZengChina Food Publishing CompanyShipin Kexue1002-66302023-09-014418586710.7506/spkx1002-6630-20221226-246Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial CommunityLI Yan, LI Wenjing, LI Chunsheng, YANG Daqiao, WANG Yueqi, WANG Di, CHEN Shengjun, WU Yanyan, LI Laihao0(1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; 2. Key Lab of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, National Research and Development Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China; 3. Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572018, China)In this study, the effect of fermentation with Bacillus subtilis B-2, a salt-tolerant strain capable of producing high yield of protease, on the microbial community and the contents of biogenic amines and amino acid nitrogen (AAN) during the fermentation of low-salt fish sauce. The results of high-throughput 16S rRNA gene sequencing showed that fermentation with B-2 reduced the richness and evenness of the microbial community, Bacillus was dominant throughout the fermentation process and the abundance of spoilage microorganisms significantly decreased. B-2 significantly inhibited the formation of histamine, putrescine, cadaverine, and tyramine, and their contents at the end of fermentation decreased by 25.9%, 35.6%, 23.6% and 9.3%, respectively, compared with those in naturally fermented fish sauce. The content of AAN in low-salt fish sauce was significantly improved by inoculated fermentation, reaching 1.23 g/100 mL after 15 days of fermentation, which was significantly higher than that of naturally fermented fish sauce (0.79 g/100 mL). The correlation network map showed that the decrease in the abundance of Brevibacterium, Dietzia, Paracoccus, Aequorivita, and Brachybacterium was the major reason for the decrease in microbial diversity at the late stage of fermentation of naturally fermented fish sauce. The abundance of Stenotrophomonas showed a significantly positive correlation with the contents of many biogenic amines in naturally fermented and B-2 fermented fish sauce, suggesting its important role in the formation of biogenic amines in low-salt fish sauce. Comparative analysis of the microbial community and quality attributes at the end of fermentation showed that the metabolism of B-2 was the main reason for the decrease in the species and abundance of spoilage microorganisms, the increase in AAN content, and the decrease in the contents of key biogenic amines. B. subtilis B-2 is expected to be developed as a special fermentation starter for fish sauce to improve the quality and safety of rapidly fermented low-salt fish sauce.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-18-008.pdflow-salt fish sauce; microbial community; biogenic amine; amino acid nitrogen; correlation network map |
| spellingShingle | LI Yan, LI Wenjing, LI Chunsheng, YANG Daqiao, WANG Yueqi, WANG Di, CHEN Shengjun, WU Yanyan, LI Laihao Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial Community Shipin Kexue low-salt fish sauce; microbial community; biogenic amine; amino acid nitrogen; correlation network map |
| title | Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial Community |
| title_full | Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial Community |
| title_fullStr | Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial Community |
| title_full_unstemmed | Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial Community |
| title_short | Bacillus subtilis B-2 Improved the Quality of Low-Salt Fermented Fish Sauce through Regulating the Microbial Community |
| title_sort | bacillus subtilis b 2 improved the quality of low salt fermented fish sauce through regulating the microbial community |
| topic | low-salt fish sauce; microbial community; biogenic amine; amino acid nitrogen; correlation network map |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-18-008.pdf |
| work_keys_str_mv | AT liyanliwenjinglichunshengyangdaqiaowangyueqiwangdichenshengjunwuyanyanlilaihao bacillussubtilisb2improvedthequalityoflowsaltfermentedfishsaucethroughregulatingthemicrobialcommunity |