Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through Exopolysaccharides
The outer membrane protein (OMP) is a kind of biofilm matrix component that widely exists in Gram-negative bacteria. However, the mechanism of OMP involved in the settlement of molluscs is still unclear. In this study, the mussel <i>Mytilus coruscus</i> was selected as a model to explore...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/8/7474 |
| _version_ | 1797605115264237568 |
|---|---|
| author | Wei Ma Xiaoyu Wang Wen Zhang Xiaomeng Hu Jin-Long Yang Xiao Liang |
| author_facet | Wei Ma Xiaoyu Wang Wen Zhang Xiaomeng Hu Jin-Long Yang Xiao Liang |
| author_sort | Wei Ma |
| collection | DOAJ |
| description | The outer membrane protein (OMP) is a kind of biofilm matrix component that widely exists in Gram-negative bacteria. However, the mechanism of OMP involved in the settlement of molluscs is still unclear. In this study, the mussel <i>Mytilus coruscus</i> was selected as a model to explore the function of <i>ompR</i>, a two-component system response regulator, on <i>Pseudoalteromonas marina</i> biofilm-forming capacity and the mussel settlement. The motility of the Δ<i>ompR</i> strain was increased, the biofilm-forming capacity was decreased, and the inducing activity of the Δ<i>ompR</i> biofilms in plantigrades decreased significantly (<i>p</i> < 0.05). The extracellular α-polysaccharide and β-polysaccharide of the Δ<i>ompR</i> strain decreased by 57.27% and 62.63%, respectively. The inactivation of the <i>ompR</i> gene decreased the <i>ompW</i> gene expression and had no impact on <i>envZ</i> expression or c-di-GMP levels. Adding recombinant OmpW protein caused the recovery of biofilm-inducing activities, accompanied by the upregulation of exopolysaccharides. The findings deepen the understanding of the regulatory mechanism of bacterial two-component systems and the settlement of benthic animals. |
| first_indexed | 2024-03-11T04:56:35Z |
| format | Article |
| id | doaj.art-e4e13769611d4cca8725dc0f1ee473c4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:56:35Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e4e13769611d4cca8725dc0f1ee473c42023-11-17T19:40:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248747410.3390/ijms24087474Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through ExopolysaccharidesWei Ma0Xiaoyu Wang1Wen Zhang2Xiaomeng Hu3Jin-Long Yang4Xiao Liang5International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaThe outer membrane protein (OMP) is a kind of biofilm matrix component that widely exists in Gram-negative bacteria. However, the mechanism of OMP involved in the settlement of molluscs is still unclear. In this study, the mussel <i>Mytilus coruscus</i> was selected as a model to explore the function of <i>ompR</i>, a two-component system response regulator, on <i>Pseudoalteromonas marina</i> biofilm-forming capacity and the mussel settlement. The motility of the Δ<i>ompR</i> strain was increased, the biofilm-forming capacity was decreased, and the inducing activity of the Δ<i>ompR</i> biofilms in plantigrades decreased significantly (<i>p</i> < 0.05). The extracellular α-polysaccharide and β-polysaccharide of the Δ<i>ompR</i> strain decreased by 57.27% and 62.63%, respectively. The inactivation of the <i>ompR</i> gene decreased the <i>ompW</i> gene expression and had no impact on <i>envZ</i> expression or c-di-GMP levels. Adding recombinant OmpW protein caused the recovery of biofilm-inducing activities, accompanied by the upregulation of exopolysaccharides. The findings deepen the understanding of the regulatory mechanism of bacterial two-component systems and the settlement of benthic animals.https://www.mdpi.com/1422-0067/24/8/7474<i>Pseudoalteromonas marina</i><i>ompR</i> geneOmpWbiofilm<i>Mytilus coruscus</i>settlement |
| spellingShingle | Wei Ma Xiaoyu Wang Wen Zhang Xiaomeng Hu Jin-Long Yang Xiao Liang Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through Exopolysaccharides International Journal of Molecular Sciences <i>Pseudoalteromonas marina</i> <i>ompR</i> gene OmpW biofilm <i>Mytilus coruscus</i> settlement |
| title | Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through Exopolysaccharides |
| title_full | Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through Exopolysaccharides |
| title_fullStr | Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through Exopolysaccharides |
| title_full_unstemmed | Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through Exopolysaccharides |
| title_short | Two-Component System Response Regulator <i>ompR</i> Regulates Mussel Settlement through Exopolysaccharides |
| title_sort | two component system response regulator i ompr i regulates mussel settlement through exopolysaccharides |
| topic | <i>Pseudoalteromonas marina</i> <i>ompR</i> gene OmpW biofilm <i>Mytilus coruscus</i> settlement |
| url | https://www.mdpi.com/1422-0067/24/8/7474 |
| work_keys_str_mv | AT weima twocomponentsystemresponseregulatoriompriregulatesmusselsettlementthroughexopolysaccharides AT xiaoyuwang twocomponentsystemresponseregulatoriompriregulatesmusselsettlementthroughexopolysaccharides AT wenzhang twocomponentsystemresponseregulatoriompriregulatesmusselsettlementthroughexopolysaccharides AT xiaomenghu twocomponentsystemresponseregulatoriompriregulatesmusselsettlementthroughexopolysaccharides AT jinlongyang twocomponentsystemresponseregulatoriompriregulatesmusselsettlementthroughexopolysaccharides AT xiaoliang twocomponentsystemresponseregulatoriompriregulatesmusselsettlementthroughexopolysaccharides |