<i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice
Lung infection can evoke pulmonary and systemic inflammation, which is associated with systemic severe symptoms, such as skeletal muscle wasting. While <i>N</i>-chlorotaurine (also known as taurine chloramine; TauCl) has anti-inflammatory effects in cells, its effects against pulmonary a...
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MDPI AG
2022-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/4/349 |
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| author | Nguyen Khanh Hoang Eiji Maegawa Shigeru Murakami Stephen W. Schaffer Takashi Ito |
| author_facet | Nguyen Khanh Hoang Eiji Maegawa Shigeru Murakami Stephen W. Schaffer Takashi Ito |
| author_sort | Nguyen Khanh Hoang |
| collection | DOAJ |
| description | Lung infection can evoke pulmonary and systemic inflammation, which is associated with systemic severe symptoms, such as skeletal muscle wasting. While <i>N</i>-chlorotaurine (also known as taurine chloramine; TauCl) has anti-inflammatory effects in cells, its effects against pulmonary and systemic inflammation after lung infection has not been elucidated. In the present study, we evaluated the anti-inflammatory effect of the taurine derivative, TauCl against <i>Escherichia coli</i>-derived lipopolysaccharide (LPS)-induced pneumonia in obese mice maintained on a high fat diet. In this study, TauCl was injected intraperitoneally 1 h before intratracheal LPS administration. While body weight was decreased by 7.5% after LPS administration, TauCl treatment suppressed body weight loss. TauCl also attenuated the increase in lung weight due to lung edema. While LPS-induced acute pneumonia caused an increase in cytokine/chemokine mRNA expression, including that of IL-1β, -6, TNF-α, MCP-1, TauCl treatment attenuated IL-6, and TNF-alpha expression, but not IL-1β and MCP-1. TauCl treatment partly attenuated the elevation of the serum cytokines. Furthermore, TauCl treatment alleviated skeletal muscle wasting. Importantly, LPS-induced expression of Atrogin-1, MuRF1 and IκB, direct or indirect targets for NFκB, were suppressed by TauCl treatment. These findings suggest that intraperitoneal TauCl treatment attenuates acute pneumonia-related pulmonary and systemic inflammation, including muscle wasting, in vivo. |
| first_indexed | 2024-03-09T13:19:53Z |
| format | Article |
| id | doaj.art-e4e97881fcab4737b1122fb56deb8a06 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T13:19:53Z |
| publishDate | 2022-04-01 |
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| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-e4e97881fcab4737b1122fb56deb8a062023-11-30T21:32:18ZengMDPI AGMetabolites2218-19892022-04-0112434910.3390/metabo12040349<i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese MiceNguyen Khanh Hoang0Eiji Maegawa1Shigeru Murakami2Stephen W. Schaffer3Takashi Ito4Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji 910-1195, JapanFaculty of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji 910-1195, JapanFaculty of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji 910-1195, JapanDepartment of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL 36688, USAFaculty of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji 910-1195, JapanLung infection can evoke pulmonary and systemic inflammation, which is associated with systemic severe symptoms, such as skeletal muscle wasting. While <i>N</i>-chlorotaurine (also known as taurine chloramine; TauCl) has anti-inflammatory effects in cells, its effects against pulmonary and systemic inflammation after lung infection has not been elucidated. In the present study, we evaluated the anti-inflammatory effect of the taurine derivative, TauCl against <i>Escherichia coli</i>-derived lipopolysaccharide (LPS)-induced pneumonia in obese mice maintained on a high fat diet. In this study, TauCl was injected intraperitoneally 1 h before intratracheal LPS administration. While body weight was decreased by 7.5% after LPS administration, TauCl treatment suppressed body weight loss. TauCl also attenuated the increase in lung weight due to lung edema. While LPS-induced acute pneumonia caused an increase in cytokine/chemokine mRNA expression, including that of IL-1β, -6, TNF-α, MCP-1, TauCl treatment attenuated IL-6, and TNF-alpha expression, but not IL-1β and MCP-1. TauCl treatment partly attenuated the elevation of the serum cytokines. Furthermore, TauCl treatment alleviated skeletal muscle wasting. Importantly, LPS-induced expression of Atrogin-1, MuRF1 and IκB, direct or indirect targets for NFκB, were suppressed by TauCl treatment. These findings suggest that intraperitoneal TauCl treatment attenuates acute pneumonia-related pulmonary and systemic inflammation, including muscle wasting, in vivo.https://www.mdpi.com/2218-1989/12/4/349taurine<i>N</i>-chlorotaurinelung inflammationcytokinemuscle wastingatrophy |
| spellingShingle | Nguyen Khanh Hoang Eiji Maegawa Shigeru Murakami Stephen W. Schaffer Takashi Ito <i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice Metabolites taurine <i>N</i>-chlorotaurine lung inflammation cytokine muscle wasting atrophy |
| title | <i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice |
| title_full | <i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice |
| title_fullStr | <i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice |
| title_full_unstemmed | <i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice |
| title_short | <i>N</i>-Chlorotaurine Reduces the Lung and Systemic Inflammation in LPS-Induced Pneumonia in High Fat Diet-Induced Obese Mice |
| title_sort | i n i chlorotaurine reduces the lung and systemic inflammation in lps induced pneumonia in high fat diet induced obese mice |
| topic | taurine <i>N</i>-chlorotaurine lung inflammation cytokine muscle wasting atrophy |
| url | https://www.mdpi.com/2218-1989/12/4/349 |
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