Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i>
Overexpression of the cytochrome P450 monooxygenase CYP392A16 has been previously associated with abamectin resistance using transcriptional analysis in the two-spotted spider mite <i>Tetranychus urticae</i>, an important pest species worldwide; however, this association has not been fun...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/12/829 |
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| author | Maria Riga Aris Ilias John Vontas Vassilis Douris |
| author_facet | Maria Riga Aris Ilias John Vontas Vassilis Douris |
| author_sort | Maria Riga |
| collection | DOAJ |
| description | Overexpression of the cytochrome P450 monooxygenase CYP392A16 has been previously associated with abamectin resistance using transcriptional analysis in the two-spotted spider mite <i>Tetranychus urticae</i>, an important pest species worldwide; however, this association has not been functionally validated in vivo despite the demonstrated ability of CYP392A16 to metabolize abamectin in vitro. We expressed CYP392A16 in vivo via a Gal4 transcription activator protein/Upstream Activating Sequence (GAL4/UAS) system in <i>Drosophila melanogaster</i> flies, driving expression with detoxification tissue-specific drivers. We demonstrated that CYP392A16 expression confers statistically significant abamectin resistance in toxicity bioassays in <i>Drosophila</i> only when its homologous redox partner, cytochrome P450 reductase (TuCPR), is co-expressed in transgenic flies. Our study shows that the <i>Drosophila</i> model can be further improved, to facilitate the functional analysis of insecticide resistance mechanisms acting alone or in combination. |
| first_indexed | 2024-03-10T14:36:25Z |
| format | Article |
| id | doaj.art-e4ed2db6d23a4585ad84811a5fb24faa |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T14:36:25Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-e4ed2db6d23a4585ad84811a5fb24faa2023-11-20T22:13:08ZengMDPI AGInsects2075-44502020-11-01111282910.3390/insects11120829Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i>Maria Riga0Aris Ilias1John Vontas2Vassilis Douris3Institute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceInstitute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceInstitute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceInstitute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceOverexpression of the cytochrome P450 monooxygenase CYP392A16 has been previously associated with abamectin resistance using transcriptional analysis in the two-spotted spider mite <i>Tetranychus urticae</i>, an important pest species worldwide; however, this association has not been functionally validated in vivo despite the demonstrated ability of CYP392A16 to metabolize abamectin in vitro. We expressed CYP392A16 in vivo via a Gal4 transcription activator protein/Upstream Activating Sequence (GAL4/UAS) system in <i>Drosophila melanogaster</i> flies, driving expression with detoxification tissue-specific drivers. We demonstrated that CYP392A16 expression confers statistically significant abamectin resistance in toxicity bioassays in <i>Drosophila</i> only when its homologous redox partner, cytochrome P450 reductase (TuCPR), is co-expressed in transgenic flies. Our study shows that the <i>Drosophila</i> model can be further improved, to facilitate the functional analysis of insecticide resistance mechanisms acting alone or in combination.https://www.mdpi.com/2075-4450/11/12/829detoxificationcytochrome P450cytochrome P450 reductaseabamectin<i>Tetranychus</i>transgenic <i>Drosophila</i> |
| spellingShingle | Maria Riga Aris Ilias John Vontas Vassilis Douris Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> Insects detoxification cytochrome P450 cytochrome P450 reductase abamectin <i>Tetranychus</i> transgenic <i>Drosophila</i> |
| title | Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
| title_full | Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
| title_fullStr | Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
| title_full_unstemmed | Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
| title_short | Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
| title_sort | co expression of a homologous cytochrome p450 reductase is required for in vivo validation of the i tetranychus urticae i cyp392a16 based abamectin resistance in i drosophila i |
| topic | detoxification cytochrome P450 cytochrome P450 reductase abamectin <i>Tetranychus</i> transgenic <i>Drosophila</i> |
| url | https://www.mdpi.com/2075-4450/11/12/829 |
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