Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638
In recent years, there has been a large increase in the amount of experimental evidence for diverse archaeal organisms, and these findings allow for a comprehensive analysis of archaeal genetic organization. However, studies about regulatory mechanisms in this cellular domain are still limited. In t...
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MDPI AG
2018-09-01
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| Series: | Life |
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| Online Access: | http://www.mdpi.com/2075-1729/8/4/40 |
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| author | Antonia Denis Mario Alberto Martínez-Núñez Silvia Tenorio-Salgado Ernesto Perez-Rueda |
| author_facet | Antonia Denis Mario Alberto Martínez-Núñez Silvia Tenorio-Salgado Ernesto Perez-Rueda |
| author_sort | Antonia Denis |
| collection | DOAJ |
| description | In recent years, there has been a large increase in the amount of experimental evidence for diverse archaeal organisms, and these findings allow for a comprehensive analysis of archaeal genetic organization. However, studies about regulatory mechanisms in this cellular domain are still limited. In this context, we identified a repertoire of 86 DNA-binding transcription factors (TFs) in the archaeon Pyrococcus furiosus DSM 3638, that are clustered into 32 evolutionary families. In structural terms, 45% of these proteins are composed of one structural domain, 41% have two domains, and 14% have three structural domains. The most abundant DNA-binding domain corresponds to the winged helix-turn-helix domain; with few alternative DNA-binding domains. We also identified seven regulons, which represent 13.5% (279 genes) of the total genes in this archaeon. These analyses increase our knowledge about gene regulation in P. furiosus DSM 3638 and provide additional clues for comprehensive modeling of transcriptional regulatory networks in the Archaea cellular domain. |
| first_indexed | 2024-04-11T11:57:57Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-04-11T11:57:57Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj.art-e4f127bac5af437a9e70754736a2cdf42022-12-22T04:25:05ZengMDPI AGLife2075-17292018-09-01844010.3390/life8040040life8040040Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638Antonia Denis0Mario Alberto Martínez-Núñez1Silvia Tenorio-Salgado2Ernesto Perez-Rueda3Facultad de Medicina, Universidad Juárez Autónoma de Tabasco, C.P. 86100 Tabasco, MexicoFacultad de Ciencias, Universidad Nacional Autónoma de México, Unidad Académica de Ciencias y Tecnología de la UNAM en Yucatán, Carretera Sierra Papacal-Chuburna Km. 5, C.P. 97302 Mérida, Yucatán, MexicoTecnológico Nacional de México, Instituto Tecnológico de Mérida, C.P. 97000 Mérida, Yucatán, MexicoInstituto de Investigaciones en Matemáticas Aplicadas y en Sistemas, Universidad Nacional Autónoma de México, Unidad Académica Yucatán, C.P. 97302 Mérida, Yucatán, MexicoIn recent years, there has been a large increase in the amount of experimental evidence for diverse archaeal organisms, and these findings allow for a comprehensive analysis of archaeal genetic organization. However, studies about regulatory mechanisms in this cellular domain are still limited. In this context, we identified a repertoire of 86 DNA-binding transcription factors (TFs) in the archaeon Pyrococcus furiosus DSM 3638, that are clustered into 32 evolutionary families. In structural terms, 45% of these proteins are composed of one structural domain, 41% have two domains, and 14% have three structural domains. The most abundant DNA-binding domain corresponds to the winged helix-turn-helix domain; with few alternative DNA-binding domains. We also identified seven regulons, which represent 13.5% (279 genes) of the total genes in this archaeon. These analyses increase our knowledge about gene regulation in P. furiosus DSM 3638 and provide additional clues for comprehensive modeling of transcriptional regulatory networks in the Archaea cellular domain.http://www.mdpi.com/2075-1729/8/4/40transcription factorsDNA-binding domainregulatory interactionarchaeonregulatory network |
| spellingShingle | Antonia Denis Mario Alberto Martínez-Núñez Silvia Tenorio-Salgado Ernesto Perez-Rueda Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638 Life transcription factors DNA-binding domain regulatory interaction archaeon regulatory network |
| title | Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638 |
| title_full | Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638 |
| title_fullStr | Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638 |
| title_full_unstemmed | Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638 |
| title_short | Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638 |
| title_sort | dissecting the repertoire of dna binding transcription factors of the archaeon pyrococcus furiosus dsm 3638 |
| topic | transcription factors DNA-binding domain regulatory interaction archaeon regulatory network |
| url | http://www.mdpi.com/2075-1729/8/4/40 |
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