Prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome
Abstract Background Growing evidence supports that extracellular vesicles (EVs) in blood plasma and other body fluids may function as biomarkers for disease. We previously found that secretory autophagosomes (SAPs), a kind of EV, could exacerbate lung injury in mice. However, the clinical value of S...
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| Format: | Article |
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BMC
2023-09-01
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| Series: | Biomarker Research |
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| Online Access: | https://doi.org/10.1186/s40364-023-00519-z |
| _version_ | 1797557568780894208 |
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| author | Xue-cheng Dong Xin-yi Xu Yue-ru Huang Xing-xing Zhu Yi Yang Wei Huang Ling Liu |
| author_facet | Xue-cheng Dong Xin-yi Xu Yue-ru Huang Xing-xing Zhu Yi Yang Wei Huang Ling Liu |
| author_sort | Xue-cheng Dong |
| collection | DOAJ |
| description | Abstract Background Growing evidence supports that extracellular vesicles (EVs) in blood plasma and other body fluids may function as biomarkers for disease. We previously found that secretory autophagosomes (SAPs), a kind of EV, could exacerbate lung injury in mice. However, the clinical value of SAPs in acute respiratory distress syndrome (ARDS), the most severe form of lung injury, remains unknown. Our study investigated the prognostic value of secretory autophagosomes in ARDS. Methods ARDS patients (n = 46) and controls (n = 8) were included in a prospective monocentric study. Bronchoalveolar lavage fluid (BALF) samples were collected from ARDS patients on the first day (Day 1) or the third day (Day 3) of enrollment and were collected from controls on Day 1. Gradient centrifugation was performed to isolate EVs. The size and concentration of EVs were characterized by nanoparticle tracking analysis (NTA). SAPs in EVs were characterized by flow cytometry, transmission electron microscopy, and western blot analysis, and the proportion of SAPs in EVs (PSV) was measured by flow cytometry. The association of SAPs with 28-day mortality was assessed. Results On Days 1 and 3, the proportion of SAPs (SAPs%) in BALF was higher in patients with ARDS than in controls. On Day 3, the SAPs% was significantly higher in nonsurvivors than in survivors. In particular, a high SAPs% was associated with poor overall survival in ARDS patients. Furthermore, the combination of SAPs% and SOFA obtained a higher predictive value of ARDS outcome than PSV or SOFA alone. Conclusion SAPs% in BALF is elevated in patients with ARDS and is associated with the risk of death in ARDS, suggesting that SAPs% may be a novel prognostic biomarker in ARDS. |
| first_indexed | 2024-03-10T17:18:43Z |
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| institution | Directory Open Access Journal |
| issn | 2050-7771 |
| language | English |
| last_indexed | 2024-03-10T17:18:43Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Biomarker Research |
| spelling | doaj.art-e4f8987a72964ae9bc0b235f34fc1eb72023-11-20T10:25:08ZengBMCBiomarker Research2050-77712023-09-011111510.1186/s40364-023-00519-zPrognostic value of secretory autophagosomes in patients with acute respiratory distress syndromeXue-cheng Dong0Xin-yi Xu1Yue-ru Huang2Xing-xing Zhu3Yi Yang4Wei Huang5Ling Liu6Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast UniversityJiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast UniversityJiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast UniversityJiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast UniversityJiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast UniversityJiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast UniversityJiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast UniversityAbstract Background Growing evidence supports that extracellular vesicles (EVs) in blood plasma and other body fluids may function as biomarkers for disease. We previously found that secretory autophagosomes (SAPs), a kind of EV, could exacerbate lung injury in mice. However, the clinical value of SAPs in acute respiratory distress syndrome (ARDS), the most severe form of lung injury, remains unknown. Our study investigated the prognostic value of secretory autophagosomes in ARDS. Methods ARDS patients (n = 46) and controls (n = 8) were included in a prospective monocentric study. Bronchoalveolar lavage fluid (BALF) samples were collected from ARDS patients on the first day (Day 1) or the third day (Day 3) of enrollment and were collected from controls on Day 1. Gradient centrifugation was performed to isolate EVs. The size and concentration of EVs were characterized by nanoparticle tracking analysis (NTA). SAPs in EVs were characterized by flow cytometry, transmission electron microscopy, and western blot analysis, and the proportion of SAPs in EVs (PSV) was measured by flow cytometry. The association of SAPs with 28-day mortality was assessed. Results On Days 1 and 3, the proportion of SAPs (SAPs%) in BALF was higher in patients with ARDS than in controls. On Day 3, the SAPs% was significantly higher in nonsurvivors than in survivors. In particular, a high SAPs% was associated with poor overall survival in ARDS patients. Furthermore, the combination of SAPs% and SOFA obtained a higher predictive value of ARDS outcome than PSV or SOFA alone. Conclusion SAPs% in BALF is elevated in patients with ARDS and is associated with the risk of death in ARDS, suggesting that SAPs% may be a novel prognostic biomarker in ARDS.https://doi.org/10.1186/s40364-023-00519-zARDSExtracellular vesiclesSecretory autophagosomesLiquid biopsyBiomarker |
| spellingShingle | Xue-cheng Dong Xin-yi Xu Yue-ru Huang Xing-xing Zhu Yi Yang Wei Huang Ling Liu Prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome Biomarker Research ARDS Extracellular vesicles Secretory autophagosomes Liquid biopsy Biomarker |
| title | Prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome |
| title_full | Prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome |
| title_fullStr | Prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome |
| title_full_unstemmed | Prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome |
| title_short | Prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome |
| title_sort | prognostic value of secretory autophagosomes in patients with acute respiratory distress syndrome |
| topic | ARDS Extracellular vesicles Secretory autophagosomes Liquid biopsy Biomarker |
| url | https://doi.org/10.1186/s40364-023-00519-z |
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