Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend?
Despite progress achieved, there is limited available information about the antibacterial activity of constituents of essential oils (EOs) from different medicinal-aromatic plants (MAPs) against fish pathogens and the complex interactions of blended EOs thereof. The present study aimed to investigat...
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MDPI AG
2021-05-01
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| author | Manolis Mandalakis Thekla I. Anastasiou Natalia Martou Sofoklis Keisaris Vasileios Greveniotis Pantelis Katharios Diamanto Lazari Nikos Krigas Efthimia Antonopoulou |
| author_facet | Manolis Mandalakis Thekla I. Anastasiou Natalia Martou Sofoklis Keisaris Vasileios Greveniotis Pantelis Katharios Diamanto Lazari Nikos Krigas Efthimia Antonopoulou |
| author_sort | Manolis Mandalakis |
| collection | DOAJ |
| description | Despite progress achieved, there is limited available information about the antibacterial activity of constituents of essential oils (EOs) from different medicinal-aromatic plants (MAPs) against fish pathogens and the complex interactions of blended EOs thereof. The present study aimed to investigate possible synergistic antimicrobial effects of EOs from seven Greek MAPs with strong potential against <i>Aeromonas veronii</i> bv. <i>sobria,</i> a fish pathogen associated with aquaculture disease outbreaks. The main objective was to evaluate whether blending of these EOs can lead to increased antimicrobial activity against the specific microorganism. A total of 127 combinations of EOs were prepared and their effect on <i>A. veronii</i> bv. <i>sobria</i> growth was tested in vitro. We examined both the inhibitory and bactericidal activities of the individual EOs and compared them to those of the blended EOs. The vast majority of the investigated combinations exhibited significant synergistic and additive effects, while antagonistic effects were evident only in a few cases, such as the mixtures containing EOs from rosemary, lemon balm and pennyroyal. The combination of EOs from Greek oregano and wild carrot, as well as the combinations of those two with Spanish oregano or savoury were the most promising ones. Overall, Greek oregano, savoury and Spanish oregano EOs were the most effective ones when applied either in pure form or blended with other EOs. |
| first_indexed | 2024-03-10T11:39:36Z |
| format | Article |
| id | doaj.art-e4fba09dac37422ca4d35bed2b20f1ab |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:39:36Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e4fba09dac37422ca4d35bed2b20f1ab2023-11-21T18:35:27ZengMDPI AGMolecules1420-30492021-05-01269273110.3390/molecules26092731Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend?Manolis Mandalakis0Thekla I. Anastasiou1Natalia Martou2Sofoklis Keisaris3Vasileios Greveniotis4Pantelis Katharios5Diamanto Lazari6Nikos Krigas7Efthimia Antonopoulou8Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, 71500 Heraklion, GreeceHellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, 71500 Heraklion, GreeceLaboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Industrial and Forage Crops, Hellenic Agricultural Organization Demeter, 41335 Larisa, GreeceHellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, 71500 Heraklion, GreeceLaboratory of Pharmacognosy, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thessaloniki, GreeceLaboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDespite progress achieved, there is limited available information about the antibacterial activity of constituents of essential oils (EOs) from different medicinal-aromatic plants (MAPs) against fish pathogens and the complex interactions of blended EOs thereof. The present study aimed to investigate possible synergistic antimicrobial effects of EOs from seven Greek MAPs with strong potential against <i>Aeromonas veronii</i> bv. <i>sobria,</i> a fish pathogen associated with aquaculture disease outbreaks. The main objective was to evaluate whether blending of these EOs can lead to increased antimicrobial activity against the specific microorganism. A total of 127 combinations of EOs were prepared and their effect on <i>A. veronii</i> bv. <i>sobria</i> growth was tested in vitro. We examined both the inhibitory and bactericidal activities of the individual EOs and compared them to those of the blended EOs. The vast majority of the investigated combinations exhibited significant synergistic and additive effects, while antagonistic effects were evident only in a few cases, such as the mixtures containing EOs from rosemary, lemon balm and pennyroyal. The combination of EOs from Greek oregano and wild carrot, as well as the combinations of those two with Spanish oregano or savoury were the most promising ones. Overall, Greek oregano, savoury and Spanish oregano EOs were the most effective ones when applied either in pure form or blended with other EOs.https://www.mdpi.com/1420-3049/26/9/2731essential oil combinationsGreek native MAPsnatural productsantimicrobial activitysynergistic effectsbacterial pathogens |
| spellingShingle | Manolis Mandalakis Thekla I. Anastasiou Natalia Martou Sofoklis Keisaris Vasileios Greveniotis Pantelis Katharios Diamanto Lazari Nikos Krigas Efthimia Antonopoulou Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend? Molecules essential oil combinations Greek native MAPs natural products antimicrobial activity synergistic effects bacterial pathogens |
| title | Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend? |
| title_full | Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend? |
| title_fullStr | Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend? |
| title_full_unstemmed | Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend? |
| title_short | Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen <i>Aeromonas veronii</i> bv. <i>sobria</i>: To Blend or Not to Blend? |
| title_sort | antibacterial effects of essential oils of seven medicinal aromatic plants against the fish pathogen i aeromonas veronii i bv i sobria i to blend or not to blend |
| topic | essential oil combinations Greek native MAPs natural products antimicrobial activity synergistic effects bacterial pathogens |
| url | https://www.mdpi.com/1420-3049/26/9/2731 |
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