Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis
Background This study was designed using a mouse model of atopic dermatitis [phthalic anhydride (PA)-treated mice], to investigate the anti-inflammatory effect of bee venom pharmacopuncture (BVP) in keratinocytes. Methods Western blot analysis was performed to investigate inflammation related protei...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MEDrang Inc.
2020-05-01
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| Series: | Journal of Acupuncture Research |
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| Online Access: | http://www.e-jar.org/upload/pdf/jar-2020-00122.pdf |
| _version_ | 1797205254204293120 |
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| author | Kyeong Ju Park Ho-Sueb Song |
| author_facet | Kyeong Ju Park Ho-Sueb Song |
| author_sort | Kyeong Ju Park |
| collection | DOAJ |
| description | Background This study was designed using a mouse model of atopic dermatitis [phthalic anhydride (PA)-treated mice], to investigate the anti-inflammatory effect of bee venom pharmacopuncture (BVP) in keratinocytes. Methods Western blot analysis was performed to investigate inflammation related protein expression of iNOS, COX-2, phospho-ERK (p-ERK), and ERK, in LPS (1 μg/mL)-activated keratinocytes, following BVP treatment, and in PA-treated mice, after BVP treatment. Griess reaction was performed to investigate NO concentration. Enzyme-linked immunosorbent assays were used to determine the concentrations of interleukin (IL)-4+, IL-17A+, IL-13 and IL-4 in PA-treated mice after BVP treatment. In addition, monocyte, macrophage, neutrophil, and eosinophil counts were measured to observe the changes in white blood cell infiltration. Results The keratinocytes of the BVP-treated group showed a decreased expression of iNOS, COX-2, ERK at 5 OX-2, ERK E, and p-ERK at 1, 2 and 5 RKRK ERK ERK, and a dose-dependent decrease in NO concentration at 2 and 5 ntrationof s. In the BVP-treated groups (0.1 μ.1-trea μ.1-treated gr), PA-treated mice showed recovery after 4 weeks which was dose-dependent, showing a significant decrease in clinical scores for AD, and a decreased concentration of IL-13 and IL-4 with BV treatment. There was a dose-dependent decrease in the infiltration of eosinophils, neutrophils, monocytes, macrophages, and a decreased thickness of the epidermis due to inflammation, and decreased expressions of iNOS, COX-2, p-ERK, ERK, especially in the 0.1 μ0/mL BVP-treated group, Conclusion These results suggest that BVP may be an effective alternative treatment for atopic dermatitis. |
| first_indexed | 2024-04-24T08:48:12Z |
| format | Article |
| id | doaj.art-e5047934058140be8c352f5d1b173975 |
| institution | Directory Open Access Journal |
| issn | 2586-288X 2586-2898 |
| language | English |
| last_indexed | 2024-04-24T08:48:12Z |
| publishDate | 2020-05-01 |
| publisher | MEDrang Inc. |
| record_format | Article |
| series | Journal of Acupuncture Research |
| spelling | doaj.art-e5047934058140be8c352f5d1b1739752024-04-16T13:02:17ZengMEDrang Inc.Journal of Acupuncture Research2586-288X2586-28982020-05-0137212312710.13045/jar.2020.001222489Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic DermatitisKyeong Ju Park0Ho-Sueb Song1 Department of Acupuncture and Moxibustion, Kyung Hee University Hospital at Gangdong, Seoul, Korea Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam, KoreaBackground This study was designed using a mouse model of atopic dermatitis [phthalic anhydride (PA)-treated mice], to investigate the anti-inflammatory effect of bee venom pharmacopuncture (BVP) in keratinocytes. Methods Western blot analysis was performed to investigate inflammation related protein expression of iNOS, COX-2, phospho-ERK (p-ERK), and ERK, in LPS (1 μg/mL)-activated keratinocytes, following BVP treatment, and in PA-treated mice, after BVP treatment. Griess reaction was performed to investigate NO concentration. Enzyme-linked immunosorbent assays were used to determine the concentrations of interleukin (IL)-4+, IL-17A+, IL-13 and IL-4 in PA-treated mice after BVP treatment. In addition, monocyte, macrophage, neutrophil, and eosinophil counts were measured to observe the changes in white blood cell infiltration. Results The keratinocytes of the BVP-treated group showed a decreased expression of iNOS, COX-2, ERK at 5 OX-2, ERK E, and p-ERK at 1, 2 and 5 RKRK ERK ERK, and a dose-dependent decrease in NO concentration at 2 and 5 ntrationof s. In the BVP-treated groups (0.1 μ.1-trea μ.1-treated gr), PA-treated mice showed recovery after 4 weeks which was dose-dependent, showing a significant decrease in clinical scores for AD, and a decreased concentration of IL-13 and IL-4 with BV treatment. There was a dose-dependent decrease in the infiltration of eosinophils, neutrophils, monocytes, macrophages, and a decreased thickness of the epidermis due to inflammation, and decreased expressions of iNOS, COX-2, p-ERK, ERK, especially in the 0.1 μ0/mL BVP-treated group, Conclusion These results suggest that BVP may be an effective alternative treatment for atopic dermatitis.http://www.e-jar.org/upload/pdf/jar-2020-00122.pdfatopic dermatitisbee venominflammationkeratinocyte |
| spellingShingle | Kyeong Ju Park Ho-Sueb Song Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis Journal of Acupuncture Research atopic dermatitis bee venom inflammation keratinocyte |
| title | Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis |
| title_full | Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis |
| title_fullStr | Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis |
| title_full_unstemmed | Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis |
| title_short | Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis |
| title_sort | effect of bee venom pharmacopuncture on inflammation in mouse model of induced atopic dermatitis |
| topic | atopic dermatitis bee venom inflammation keratinocyte |
| url | http://www.e-jar.org/upload/pdf/jar-2020-00122.pdf |
| work_keys_str_mv | AT kyeongjupark effectofbeevenompharmacopunctureoninflammationinmousemodelofinducedatopicdermatitis AT hosuebsong effectofbeevenompharmacopunctureoninflammationinmousemodelofinducedatopicdermatitis |