Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish
In recent decades, zebrafish (<i>Danio rerio</i>) has become a widely used vertebrate animal model for studying development and human diseases. However, studies on skin medication using zebrafish are rare. Here, we developed a novel protocol for percutaneous absorption of molecules via t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/17/3974 |
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| author | Daizo Morikane Liqing Zang Norihiro Nishimura |
| author_facet | Daizo Morikane Liqing Zang Norihiro Nishimura |
| author_sort | Daizo Morikane |
| collection | DOAJ |
| description | In recent decades, zebrafish (<i>Danio rerio</i>) has become a widely used vertebrate animal model for studying development and human diseases. However, studies on skin medication using zebrafish are rare. Here, we developed a novel protocol for percutaneous absorption of molecules via the zebrafish tail skin, by applying a liquid solution directly, or using a filter paper imbibed with a chemical solution (coating). Human skin is capable of absorbing felbinac and loxoprofen sodium hydrate (LSH), but not glycyrrhetinic acid (GA) and terbinafine hydrochloride (TH). To evaluate the possibility and the quality of transdermal absorption in zebrafish, we transdermally administered these four drugs to zebrafish. Pharmacokinetics showed that felbinac was present in the blood of zebrafish subjected to all administration methods. Felbinac blood concentrations peaked at 2 h and disappeared 7 h after administration. GA was not detected following transdermal administrations, but was following exposure. LSH was not found in the circulatory system after transdermal administration, but TH was. A dose-response correlation was observed for felbinac blood concentration. These findings suggest that zebrafish are capable of absorbing drug molecules through their skin. However, the present data cannot demonstrate that zebrafish is a practical model to predict human skin absorption. Further systemic studies are needed to observe the correlations in percutaneous absorption between humans and zebrafish. |
| first_indexed | 2024-03-10T16:41:18Z |
| format | Article |
| id | doaj.art-e5050851df4d46c9bbdde8c736228a2c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:41:18Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e5050851df4d46c9bbdde8c736228a2c2023-11-20T12:01:21ZengMDPI AGMolecules1420-30492020-08-012517397410.3390/molecules25173974Evaluation of the Percutaneous Absorption of Drug Molecules in ZebrafishDaizo Morikane0Liqing Zang1Norihiro Nishimura2DIA Pharmaceutical Co., Ltd., Kashihara, Nara 634-0803, JapanGraduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, JapanGraduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, JapanIn recent decades, zebrafish (<i>Danio rerio</i>) has become a widely used vertebrate animal model for studying development and human diseases. However, studies on skin medication using zebrafish are rare. Here, we developed a novel protocol for percutaneous absorption of molecules via the zebrafish tail skin, by applying a liquid solution directly, or using a filter paper imbibed with a chemical solution (coating). Human skin is capable of absorbing felbinac and loxoprofen sodium hydrate (LSH), but not glycyrrhetinic acid (GA) and terbinafine hydrochloride (TH). To evaluate the possibility and the quality of transdermal absorption in zebrafish, we transdermally administered these four drugs to zebrafish. Pharmacokinetics showed that felbinac was present in the blood of zebrafish subjected to all administration methods. Felbinac blood concentrations peaked at 2 h and disappeared 7 h after administration. GA was not detected following transdermal administrations, but was following exposure. LSH was not found in the circulatory system after transdermal administration, but TH was. A dose-response correlation was observed for felbinac blood concentration. These findings suggest that zebrafish are capable of absorbing drug molecules through their skin. However, the present data cannot demonstrate that zebrafish is a practical model to predict human skin absorption. Further systemic studies are needed to observe the correlations in percutaneous absorption between humans and zebrafish.https://www.mdpi.com/1420-3049/25/17/3974drug screeningfelbinacglycyrrhetinic acidpharmacokineticszebrafish |
| spellingShingle | Daizo Morikane Liqing Zang Norihiro Nishimura Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish Molecules drug screening felbinac glycyrrhetinic acid pharmacokinetics zebrafish |
| title | Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish |
| title_full | Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish |
| title_fullStr | Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish |
| title_full_unstemmed | Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish |
| title_short | Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish |
| title_sort | evaluation of the percutaneous absorption of drug molecules in zebrafish |
| topic | drug screening felbinac glycyrrhetinic acid pharmacokinetics zebrafish |
| url | https://www.mdpi.com/1420-3049/25/17/3974 |
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