Separation and size determination of human serum lipoproteins by agarose gel filtration
A method is described for the separation of the three major classes of human serum lipoproteins by gel filtration on columns of 4 and 6% agarose gel. After calibration of the columns, the elution volumes of the lipoproteins were used to calculate the molecular sizes and molecular weights of these ma...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1967-09-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520389082 |
| _version_ | 1818672934330302464 |
|---|---|
| author | Simeon Margolis |
| author_facet | Simeon Margolis |
| author_sort | Simeon Margolis |
| collection | DOAJ |
| description | A method is described for the separation of the three major classes of human serum lipoproteins by gel filtration on columns of 4 and 6% agarose gel. After calibration of the columns, the elution volumes of the lipoproteins were used to calculate the molecular sizes and molecular weights of these macromolecules. The technique was employed to demonstrate aggregation of low density lipoprotein following partial delipidation, partial proteolysis, or mild heat denaturation. Agarose gel filtration shows promise as a useful method for the isolation, purification, and characterization of lipoproteins. |
| first_indexed | 2024-12-17T07:47:47Z |
| format | Article |
| id | doaj.art-e506c4cc7e2545df8157c2737a4ba122 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T07:47:47Z |
| publishDate | 1967-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e506c4cc7e2545df8157c2737a4ba1222022-12-21T21:57:57ZengElsevierJournal of Lipid Research0022-22751967-09-0185501507Separation and size determination of human serum lipoproteins by agarose gel filtrationSimeon Margolis0Departments of Medicine and Physiological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205A method is described for the separation of the three major classes of human serum lipoproteins by gel filtration on columns of 4 and 6% agarose gel. After calibration of the columns, the elution volumes of the lipoproteins were used to calculate the molecular sizes and molecular weights of these macromolecules. The technique was employed to demonstrate aggregation of low density lipoprotein following partial delipidation, partial proteolysis, or mild heat denaturation. Agarose gel filtration shows promise as a useful method for the isolation, purification, and characterization of lipoproteins.http://www.sciencedirect.com/science/article/pii/S0022227520389082gel filtrationserumlipoproteinsagaroseseparationmolecular weight |
| spellingShingle | Simeon Margolis Separation and size determination of human serum lipoproteins by agarose gel filtration Journal of Lipid Research gel filtration serum lipoproteins agarose separation molecular weight |
| title | Separation and size determination of human serum lipoproteins by agarose gel filtration |
| title_full | Separation and size determination of human serum lipoproteins by agarose gel filtration |
| title_fullStr | Separation and size determination of human serum lipoproteins by agarose gel filtration |
| title_full_unstemmed | Separation and size determination of human serum lipoproteins by agarose gel filtration |
| title_short | Separation and size determination of human serum lipoproteins by agarose gel filtration |
| title_sort | separation and size determination of human serum lipoproteins by agarose gel filtration |
| topic | gel filtration serum lipoproteins agarose separation molecular weight |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520389082 |
| work_keys_str_mv | AT simeonmargolis separationandsizedeterminationofhumanserumlipoproteinsbyagarosegelfiltration |