Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior
Innovative engineering design for biologically active hydroxyapatites requires enhancing both mechanical and physical properties, along with biocompatibility, by doping with appropriate chemical elements. Herein, the purpose of this investigation was to evaluate and elucidate the model of naturally...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/2/802 |
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| author | Malgorzata Tyszka-Czochara Marzena Suder Agnieszka Dołhańczuk-Śródka Małgorzata Rajfur Katarzyna Grata Michał Starosta Agnieszka Jagoda-Pasternak Wiktor Kasprzyk Anna K. Nowak Saeid Ahmadzadeh Dorota Kopeć Piotr Suryło Tomasz Świergosz Katarzyna M. Stadnicka |
| author_facet | Malgorzata Tyszka-Czochara Marzena Suder Agnieszka Dołhańczuk-Śródka Małgorzata Rajfur Katarzyna Grata Michał Starosta Agnieszka Jagoda-Pasternak Wiktor Kasprzyk Anna K. Nowak Saeid Ahmadzadeh Dorota Kopeć Piotr Suryło Tomasz Świergosz Katarzyna M. Stadnicka |
| author_sort | Malgorzata Tyszka-Czochara |
| collection | DOAJ |
| description | Innovative engineering design for biologically active hydroxyapatites requires enhancing both mechanical and physical properties, along with biocompatibility, by doping with appropriate chemical elements. Herein, the purpose of this investigation was to evaluate and elucidate the model of naturally occurring hydroxyapatite and the effects of doped trace elements on the function of normal human fibroblasts, representing the main cells of connective tissues. The substrates applied (geological apatites with hexagonal prismatic crystal habit originated from Slyudyanka, Lake Baikal, Russia (GAp) and from Imilchil, The Atlas Mountains, Morocco (YAp)) were prepared from mineral natural apatite with a chemical composition consistent with the building blocks of enamel and enriched with a significant F<sup>−</sup> content. Materials in the form of powders, extracts and single-crystal plates have been investigated. Moreover, the effects on the function of fibroblasts cultured on the analyzed surfaces in the form of changes in metabolic activity, proliferation and cell morphology were evaluated. Apatite plates were also evaluated for cytotoxicity and immune cell activation capacity. The results suggest that a moderate amount of F<sup>−</sup> has a positive effect on cell proliferation, whereas an inhibitory effect was attributed to the Cl<sup>−</sup> concentration. It was found that for (100) GAp plate, fibroblast proliferation was significantly increased, whereas for (001) YAp plate, it was significantly reduced, with no cytotoxic effect and no immune response from macrophages exposed to these materials. The study of the interaction of fibroblasts with apatite crystal surfaces provides a characterization relevant to medical applications and may contribute to the design of biomaterials suitable for medical applications and the evaluation of their bioavailability. |
| first_indexed | 2024-03-10T01:18:33Z |
| format | Article |
| id | doaj.art-e50c56134ce544d9bfa49416907a0774 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:18:33Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e50c56134ce544d9bfa49416907a07742023-11-23T14:04:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-0123280210.3390/ijms23020802Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast BehaviorMalgorzata Tyszka-Czochara0Marzena Suder1Agnieszka Dołhańczuk-Śródka2Małgorzata Rajfur3Katarzyna Grata4Michał Starosta5Agnieszka Jagoda-Pasternak6Wiktor Kasprzyk7Anna K. Nowak8Saeid Ahmadzadeh9Dorota Kopeć10Piotr Suryło11Tomasz Świergosz12Katarzyna M. Stadnicka13Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, PolandDepartment of Crystal Chemistry and Crystal Physics, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandInstitute of Environmental Engineering and Biotechnology, University of Opole, Kominka 6, 45-035 Opole, PolandInstitute of Environmental Engineering and Biotechnology, University of Opole, Kominka 6, 45-035 Opole, PolandInstitute of Environmental Engineering and Biotechnology, University of Opole, Kominka 6, 45-035 Opole, PolandInstitute of Environmental Engineering and Biotechnology, University of Opole, Kominka 6, 45-035 Opole, PolandDepartment of General and Inorganic Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, PolandDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, PolandDepartment of General and Inorganic Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, PolandPharmaceutics Research Centre, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman 7616913555, IranDepartment of Chemical Technology and Environmental Analysis, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, PolandDepartment of Chemical Technology and Environmental Analysis, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, PolandDepartment of Chemical Technology and Environmental Analysis, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, PolandDepartment of Crystal Chemistry and Crystal Physics, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, PolandInnovative engineering design for biologically active hydroxyapatites requires enhancing both mechanical and physical properties, along with biocompatibility, by doping with appropriate chemical elements. Herein, the purpose of this investigation was to evaluate and elucidate the model of naturally occurring hydroxyapatite and the effects of doped trace elements on the function of normal human fibroblasts, representing the main cells of connective tissues. The substrates applied (geological apatites with hexagonal prismatic crystal habit originated from Slyudyanka, Lake Baikal, Russia (GAp) and from Imilchil, The Atlas Mountains, Morocco (YAp)) were prepared from mineral natural apatite with a chemical composition consistent with the building blocks of enamel and enriched with a significant F<sup>−</sup> content. Materials in the form of powders, extracts and single-crystal plates have been investigated. Moreover, the effects on the function of fibroblasts cultured on the analyzed surfaces in the form of changes in metabolic activity, proliferation and cell morphology were evaluated. Apatite plates were also evaluated for cytotoxicity and immune cell activation capacity. The results suggest that a moderate amount of F<sup>−</sup> has a positive effect on cell proliferation, whereas an inhibitory effect was attributed to the Cl<sup>−</sup> concentration. It was found that for (100) GAp plate, fibroblast proliferation was significantly increased, whereas for (001) YAp plate, it was significantly reduced, with no cytotoxic effect and no immune response from macrophages exposed to these materials. The study of the interaction of fibroblasts with apatite crystal surfaces provides a characterization relevant to medical applications and may contribute to the design of biomaterials suitable for medical applications and the evaluation of their bioavailability.https://www.mdpi.com/1422-0067/23/2/802hydroxyapatitemineral apatite single crystalsFTIRSEM-EDXSX-ray diffractionfibroblast cell culture |
| spellingShingle | Malgorzata Tyszka-Czochara Marzena Suder Agnieszka Dołhańczuk-Śródka Małgorzata Rajfur Katarzyna Grata Michał Starosta Agnieszka Jagoda-Pasternak Wiktor Kasprzyk Anna K. Nowak Saeid Ahmadzadeh Dorota Kopeć Piotr Suryło Tomasz Świergosz Katarzyna M. Stadnicka Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior International Journal of Molecular Sciences hydroxyapatite mineral apatite single crystals FTIR SEM-EDXS X-ray diffraction fibroblast cell culture |
| title | Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior |
| title_full | Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior |
| title_fullStr | Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior |
| title_full_unstemmed | Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior |
| title_short | Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior |
| title_sort | nature inspired effects of naturally occurring trace element doped hydroxyapatite combined with surface interactions of mineral apatite single crystals on human fibroblast behavior |
| topic | hydroxyapatite mineral apatite single crystals FTIR SEM-EDXS X-ray diffraction fibroblast cell culture |
| url | https://www.mdpi.com/1422-0067/23/2/802 |
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