Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis
The immune-modulatory effects of black seeds (Nigella sativa seeds, NSS) are well documented, but the overall in vivo impact of this important natural medicinal product on immune system function has yet to be established. Here we systematically reviewed and meta-analyzed the effects of NSS on humora...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024034212 |
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| author | Abdulsamad Alsalahi Nian N.N. Maarof Mohammed A. Alshawsh Musheer A. Aljaberi Mousa A. Qasem Abdulaleem Mahuob Nassrin A. Badroon Ebthag A.M. Mussa Rukman A. Hamat Atiyah M. Abdallah |
| author_facet | Abdulsamad Alsalahi Nian N.N. Maarof Mohammed A. Alshawsh Musheer A. Aljaberi Mousa A. Qasem Abdulaleem Mahuob Nassrin A. Badroon Ebthag A.M. Mussa Rukman A. Hamat Atiyah M. Abdallah |
| author_sort | Abdulsamad Alsalahi |
| collection | DOAJ |
| description | The immune-modulatory effects of black seeds (Nigella sativa seeds, NSS) are well documented, but the overall in vivo impact of this important natural medicinal product on immune system function has yet to be established. Here we systematically reviewed and meta-analyzed the effects of NSS on humoral [serum titers of immunoglobulins including IgG, IgM, anti-Newcastle virus disease (anti-NDV), and sheep red blood cell antigen (anti-SRBC)] and cellular immunity [total white blood cell (WBC) count and percentages of monocytes, lymphocytes, basophils, neutrophils, and eosinophils] in healthy animals. The PubMed, ScienceDirect, Web of Science, and Scopus databases were searched according to predefined eligibility criteria. Meta-analyses were performed to estimate the final effect size using RevMan software. Seventeen animal studies were eligible for analysis. For humoral immunity, the overall pooled effect size (ES) of NSS on serum titers of IgM and anti-NVD antibodies was not significantly different [mean difference (MD) 75.27, 95% CI: −44.76 to 195.30, p = 0.22 (I2 = 89%, p = 0.003), and −0.01, 95% CI: −0.27 to 0.25, p = 0.94 (I2 = 74%, p = 0.02), respectively]. However, NSS significantly increased serum titers of IgG and anti-SRBC antibodies [MD 3.30, 95% CI: 2.27 to 4.32, p = 0.00001 (I2 = 0%, p = 0.97), and 1.15, 95% CI: 0.74 to 1.56, p = 0.00001 (I2 = 0%, p = 0.43), respectively]. For cellular immunity, the ES of NSS on WBCs, monocytes, and lymphocytes were not significantly different [MD 0.29, 95% CI: −0.55 to 1.13, p = 0.50, (I2 = 14%, p = 0.32), - 0.01, 95% CI: −0.45 to 0.44, p = 0.97 (I2 = 0%, p = 0.77), and 4.73, 95% CI: −7.13 to 16.59, p = 0.43, (I2 = 99%, p = 0.00001), respectively]. In conclusion, black seeds enhance humoral immunity in healthy animals but do not affect cellular immunity. |
| first_indexed | 2024-04-24T13:50:55Z |
| format | Article |
| id | doaj.art-e50d19feb5b44d86aaf9e6e37ea12d3b |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:50:55Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e50d19feb5b44d86aaf9e6e37ea12d3b2024-04-04T05:04:45ZengElsevierHeliyon2405-84402024-03-01106e27390Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysisAbdulsamad Alsalahi0Nian N.N. Maarof1Mohammed A. Alshawsh2Musheer A. Aljaberi3Mousa A. Qasem4Abdulaleem Mahuob5Nassrin A. Badroon6Ebthag A.M. Mussa7Rukman A. Hamat8Atiyah M. Abdallah9Department of Pharmacology, Faculty of Pharmacy, Sana'a University, Mazbah District, 1247, Sana'a Secretariat, Yemen; Corresponding author.Department of Chemistry, College of Education, University of Sulaimani, 46001 Sulaimani, Kurdistan, IraqDepartment of Pharmacology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia; Department of Paediatrics, School of Clinical Sciences, Faculty of Medicine, Nursing and Health Sciences, Monash University, 246 Clayton Road, Clayton, VIC, 3168, AustraliaDepartment of Community Health, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43300 Selangor, MalaysiaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Universiti Malaya, 50603 Kuala Lumpur, MalaysiaClinical Pharmacy, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, MalaysiaSpecial Infectious Agent Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaDepartment of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaDepartment of Biomedical Science, College of Health Science, Qatar University, 2713 Doha, Qatar; Corresponding author.The immune-modulatory effects of black seeds (Nigella sativa seeds, NSS) are well documented, but the overall in vivo impact of this important natural medicinal product on immune system function has yet to be established. Here we systematically reviewed and meta-analyzed the effects of NSS on humoral [serum titers of immunoglobulins including IgG, IgM, anti-Newcastle virus disease (anti-NDV), and sheep red blood cell antigen (anti-SRBC)] and cellular immunity [total white blood cell (WBC) count and percentages of monocytes, lymphocytes, basophils, neutrophils, and eosinophils] in healthy animals. The PubMed, ScienceDirect, Web of Science, and Scopus databases were searched according to predefined eligibility criteria. Meta-analyses were performed to estimate the final effect size using RevMan software. Seventeen animal studies were eligible for analysis. For humoral immunity, the overall pooled effect size (ES) of NSS on serum titers of IgM and anti-NVD antibodies was not significantly different [mean difference (MD) 75.27, 95% CI: −44.76 to 195.30, p = 0.22 (I2 = 89%, p = 0.003), and −0.01, 95% CI: −0.27 to 0.25, p = 0.94 (I2 = 74%, p = 0.02), respectively]. However, NSS significantly increased serum titers of IgG and anti-SRBC antibodies [MD 3.30, 95% CI: 2.27 to 4.32, p = 0.00001 (I2 = 0%, p = 0.97), and 1.15, 95% CI: 0.74 to 1.56, p = 0.00001 (I2 = 0%, p = 0.43), respectively]. For cellular immunity, the ES of NSS on WBCs, monocytes, and lymphocytes were not significantly different [MD 0.29, 95% CI: −0.55 to 1.13, p = 0.50, (I2 = 14%, p = 0.32), - 0.01, 95% CI: −0.45 to 0.44, p = 0.97 (I2 = 0%, p = 0.77), and 4.73, 95% CI: −7.13 to 16.59, p = 0.43, (I2 = 99%, p = 0.00001), respectively]. In conclusion, black seeds enhance humoral immunity in healthy animals but do not affect cellular immunity.http://www.sciencedirect.com/science/article/pii/S2405844024034212ImmunityCellularHumeralThymoquinoneNigella sativaBlack seeds |
| spellingShingle | Abdulsamad Alsalahi Nian N.N. Maarof Mohammed A. Alshawsh Musheer A. Aljaberi Mousa A. Qasem Abdulaleem Mahuob Nassrin A. Badroon Ebthag A.M. Mussa Rukman A. Hamat Atiyah M. Abdallah Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis Heliyon Immunity Cellular Humeral Thymoquinone Nigella sativa Black seeds |
| title | Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis |
| title_full | Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis |
| title_fullStr | Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis |
| title_full_unstemmed | Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis |
| title_short | Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis |
| title_sort | immune stimulatory effect of nigella sativa in healthy animal models a systematic review and meta analysis |
| topic | Immunity Cellular Humeral Thymoquinone Nigella sativa Black seeds |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024034212 |
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