A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination
A novel and sensitive fluorescence immunoassay was developed for the determination of Vibrio parahaemolyticus (VP) based on gold nanoclusters (BSA-AuNCs). The assay signal was originated from inner filter effect (IFE) between BSA-AuNCs and p-nitrophenol (PNP). A double emission peak fluorescent gold...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715621001132 |
| _version_ | 1818871817618587648 |
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| author | Guangxin Yang Chunhao Wei Yunyu Tang Jingru Zhang Xuan Zhang Hai Chi Leren Tao Cong Kong |
| author_facet | Guangxin Yang Chunhao Wei Yunyu Tang Jingru Zhang Xuan Zhang Hai Chi Leren Tao Cong Kong |
| author_sort | Guangxin Yang |
| collection | DOAJ |
| description | A novel and sensitive fluorescence immunoassay was developed for the determination of Vibrio parahaemolyticus (VP) based on gold nanoclusters (BSA-AuNCs). The assay signal was originated from inner filter effect (IFE) between BSA-AuNCs and p-nitrophenol (PNP). A double emission peak fluorescent gold nanocluster with good property was quickly synthesized, which was used as fluorescent probe in this method. To achieve the IFE, Alkaline phosphatase labeled second antibody (IgG-ALP), which could generate PNP with the substrate of PNPP (p-nitrophenyl phosphate), was used in the immune reaction. According the competitive reaction, VP could be detected indirectly by the change of fluorescence signal. Under the optimal conditions, the limit of detection (LOD) for VP was 5 × 102 CFU/mL. The linear detection range was 103-107 CFU/mL. Furthermore, the proposed method was successfully applied to detect VP in real samples, giving recoveries in the range of 82.5%–113.0%, and the coefficient of variation was 5.46%–9.88%. |
| first_indexed | 2024-12-19T12:28:57Z |
| format | Article |
| id | doaj.art-e51030c76b37476daa7f05922871e50c |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-12-19T12:28:57Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-e51030c76b37476daa7f05922871e50c2022-12-21T20:21:27ZengElsevierResults in Chemistry2211-71562021-01-013100208A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determinationGuangxin Yang0Chunhao Wei1Yunyu Tang2Jingru Zhang3Xuan Zhang4Hai Chi5Leren Tao6Cong Kong7East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100000, China; Corresponding authors.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100000, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Corresponding authors.School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; Corresponding authors.A novel and sensitive fluorescence immunoassay was developed for the determination of Vibrio parahaemolyticus (VP) based on gold nanoclusters (BSA-AuNCs). The assay signal was originated from inner filter effect (IFE) between BSA-AuNCs and p-nitrophenol (PNP). A double emission peak fluorescent gold nanocluster with good property was quickly synthesized, which was used as fluorescent probe in this method. To achieve the IFE, Alkaline phosphatase labeled second antibody (IgG-ALP), which could generate PNP with the substrate of PNPP (p-nitrophenyl phosphate), was used in the immune reaction. According the competitive reaction, VP could be detected indirectly by the change of fluorescence signal. Under the optimal conditions, the limit of detection (LOD) for VP was 5 × 102 CFU/mL. The linear detection range was 103-107 CFU/mL. Furthermore, the proposed method was successfully applied to detect VP in real samples, giving recoveries in the range of 82.5%–113.0%, and the coefficient of variation was 5.46%–9.88%.http://www.sciencedirect.com/science/article/pii/S2211715621001132Vibrio parahaemolyticusGold nanoclustersFluorescence immunoassayInner Filter Effect |
| spellingShingle | Guangxin Yang Chunhao Wei Yunyu Tang Jingru Zhang Xuan Zhang Hai Chi Leren Tao Cong Kong A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination Results in Chemistry Vibrio parahaemolyticus Gold nanoclusters Fluorescence immunoassay Inner Filter Effect |
| title | A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination |
| title_full | A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination |
| title_fullStr | A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination |
| title_full_unstemmed | A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination |
| title_short | A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination |
| title_sort | novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for vibrio parahaemolyticus determination |
| topic | Vibrio parahaemolyticus Gold nanoclusters Fluorescence immunoassay Inner Filter Effect |
| url | http://www.sciencedirect.com/science/article/pii/S2211715621001132 |
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