Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLC
Indoxyl sulfate (IS) is a protein-bound typical uremic toxin that accumulates in patients with impaired kidney function. The laboratory methods used to quantify serum concentrations of IS require improvement. We report an optimal, cost-effective alternative to the methods currently used.The methods...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Korean Society of Nephrology
2012-06-01
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| Series: | Kidney Research and Clinical Practice |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211913212003968 |
| _version_ | 1828763018301800448 |
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| author | Chuan Zou Fuhua Lu Yuchi Wu Qizhan Lin Xusheng Liu |
| author_facet | Chuan Zou Fuhua Lu Yuchi Wu Qizhan Lin Xusheng Liu |
| author_sort | Chuan Zou |
| collection | DOAJ |
| description | Indoxyl sulfate (IS) is a protein-bound typical uremic toxin that accumulates in patients with impaired kidney function. The laboratory methods used to quantify serum concentrations of IS require improvement. We report an optimal, cost-effective alternative to the methods currently used.The methods is as follows: serum samples were extracted and deproteinized using acetonitrile and then further purified using dichloromethane. The samples were then analyzed using high-performance liquid chromatography (HPLC) with a fluorescence detector at a flow rate of 1.0 mL/min. The isocratic mobile phase consisted of acetonitrile−0.2% (V/V) trifluoroacetic acid in phosphate buffered saline, pH 2.5 (8:92, V/V). Detector settings were λex 280 nm/λem 390 nm. Under the indicated conditions, IS was well separated. The retention time was approximately 6.59±0.11 min. The results of the precision and reproducibility tests were <3%, and the recovery rate was greater than 95%,(98.44±3.21%).
In conclusion, HPLC with a fluorescence detector is a simple, sensitive, and reliable method for quantifying IS concentration in the serum of patients with chronic renal failure. |
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| format | Article |
| id | doaj.art-e511853bae934cf4a50badecfc21ebf8 |
| institution | Directory Open Access Journal |
| issn | 2211-9132 |
| language | English |
| last_indexed | 2024-12-11T01:52:58Z |
| publishDate | 2012-06-01 |
| publisher | The Korean Society of Nephrology |
| record_format | Article |
| series | Kidney Research and Clinical Practice |
| spelling | doaj.art-e511853bae934cf4a50badecfc21ebf82022-12-22T01:24:41ZengThe Korean Society of NephrologyKidney Research and Clinical Practice2211-91322012-06-01312A2610.1016/j.krcp.2012.04.363Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLCChuan ZouFuhua LuYuchi WuQizhan LinXusheng LiuIndoxyl sulfate (IS) is a protein-bound typical uremic toxin that accumulates in patients with impaired kidney function. The laboratory methods used to quantify serum concentrations of IS require improvement. We report an optimal, cost-effective alternative to the methods currently used.The methods is as follows: serum samples were extracted and deproteinized using acetonitrile and then further purified using dichloromethane. The samples were then analyzed using high-performance liquid chromatography (HPLC) with a fluorescence detector at a flow rate of 1.0 mL/min. The isocratic mobile phase consisted of acetonitrile−0.2% (V/V) trifluoroacetic acid in phosphate buffered saline, pH 2.5 (8:92, V/V). Detector settings were λex 280 nm/λem 390 nm. Under the indicated conditions, IS was well separated. The retention time was approximately 6.59±0.11 min. The results of the precision and reproducibility tests were <3%, and the recovery rate was greater than 95%,(98.44±3.21%). In conclusion, HPLC with a fluorescence detector is a simple, sensitive, and reliable method for quantifying IS concentration in the serum of patients with chronic renal failure.http://www.sciencedirect.com/science/article/pii/S2211913212003968 |
| spellingShingle | Chuan Zou Fuhua Lu Yuchi Wu Qizhan Lin Xusheng Liu Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLC Kidney Research and Clinical Practice |
| title | Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLC |
| title_full | Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLC |
| title_fullStr | Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLC |
| title_full_unstemmed | Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLC |
| title_short | Indoxyl Sulfate Serum Level in Chronic Renal Failure Patients detected using Fluorescence-HPLC |
| title_sort | indoxyl sulfate serum level in chronic renal failure patients detected using fluorescence hplc |
| url | http://www.sciencedirect.com/science/article/pii/S2211913212003968 |
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