CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
Abstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogen...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-08596-5 |
| _version_ | 1819127284884307968 |
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| author | Jun Wu Marcela Vilarino Keiichiro Suzuki Daiji Okamura Yanina Soledad Bogliotti Insung Park Joan Rowe Bret McNabb Pablo Juan Ross Juan Carlos Izpisua Belmonte |
| author_facet | Jun Wu Marcela Vilarino Keiichiro Suzuki Daiji Okamura Yanina Soledad Bogliotti Insung Park Joan Rowe Bret McNabb Pablo Juan Ross Juan Carlos Izpisua Belmonte |
| author_sort | Jun Wu |
| collection | DOAJ |
| description | Abstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs. |
| first_indexed | 2024-12-22T08:09:29Z |
| format | Article |
| id | doaj.art-e5179872561d480fb6dfc184638202f4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T08:09:29Z |
| publishDate | 2017-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e5179872561d480fb6dfc184638202f42022-12-21T18:33:04ZengNature PortfolioScientific Reports2045-23222017-09-01711610.1038/s41598-017-08596-5CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigsJun Wu0Marcela Vilarino1Keiichiro Suzuki2Daiji Okamura3Yanina Soledad Bogliotti4Insung Park5Joan Rowe6Bret McNabb7Pablo Juan Ross8Juan Carlos Izpisua Belmonte9Salk Institute for Biological StudiesDepartment of Animal Science, University of California DavisSalk Institute for Biological StudiesUniversidad Católica San Antonio de MurciaDepartment of Animal Science, University of California DavisDepartment of Animal Science, University of California DavisSchool of Veterinary Medicine, University of California DavisSchool of Veterinary Medicine, University of California DavisDepartment of Animal Science, University of California DavisSalk Institute for Biological StudiesAbstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.https://doi.org/10.1038/s41598-017-08596-5 |
| spellingShingle | Jun Wu Marcela Vilarino Keiichiro Suzuki Daiji Okamura Yanina Soledad Bogliotti Insung Park Joan Rowe Bret McNabb Pablo Juan Ross Juan Carlos Izpisua Belmonte CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs Scientific Reports |
| title | CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs |
| title_full | CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs |
| title_fullStr | CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs |
| title_full_unstemmed | CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs |
| title_short | CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs |
| title_sort | crispr cas9 mediated one step disabling of pancreatogenesis in pigs |
| url | https://doi.org/10.1038/s41598-017-08596-5 |
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