Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seeds
Abstract Sect. tuberculata plant belongs to the Camellia genus and is named for the “tuberculiform protuberance on the surface of the ovary and fruit”. It is a species of great ornamental value and potential medicinal value. However, little has been reported on the metabolites of C. tuberculata seed...
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| Format: | Article |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-58725-0 |
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| author | Zhaohui Ran Zhi Li Xu Xiao Chao Yan Mingtai An Juyan Chen Ming Tang |
| author_facet | Zhaohui Ran Zhi Li Xu Xiao Chao Yan Mingtai An Juyan Chen Ming Tang |
| author_sort | Zhaohui Ran |
| collection | DOAJ |
| description | Abstract Sect. tuberculata plant belongs to the Camellia genus and is named for the “tuberculiform protuberance on the surface of the ovary and fruit”. It is a species of great ornamental value and potential medicinal value. However, little has been reported on the metabolites of C. tuberculata seeds. Therefore, this study was conducted to investigate the metabolites of C. tuberculata seeds based on UPLC/ESI-Q TRAP-MS/MS with extensively targeted metabolomics. A total of 1611 metabolites were identified, including 107 alkaloids, 276 amino acids and derivatives, 283 flavonoids, 86 lignans and coumarins, 181 lipids, 68 nucleotides and derivatives, 101 organic acids, 190 phenolic acids, 10 quinones, 4 steroids, 17 tannins, 111 terpenoids, and 177 other metabolites. We compared the different metabolites in seeds between HKH, ZM, ZY, and LY. The 1311 identified different metabolites were classified into three categories. Sixty-three overlapping significant different metabolites were found, of which lignans and coumarins accounted for the largest proportion. The differentially accumulated metabolites were enriched in different metabolic pathways between HKH vs. LY, HKH vs. ZM, HKH vs. ZY, LY vs. ZY, ZM vs. LY and ZM vs. ZY, with the most abundant metabolic pathways being 4, 2, 4, 7, 7 and 5, respectively (p < 0.05). Moreover, among the top 20 metabolites in each subgroup comparison in terms of difference multiplicity 7, 8 and 13. ZM and ZY had the highest phenolic acid content. Ninety-six disease-resistant metabolites and 48 major traditional Chinese medicine agents were identified based on seven diseases. The results of this study will not only lead to a more comprehensive and in-depth understanding of the metabolic properties of C. tuberculata seeds, but also provide a scientific basis for the excavation and further development of its medicinal value. |
| first_indexed | 2024-04-24T07:16:51Z |
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| language | English |
| last_indexed | 2024-04-24T07:16:51Z |
| publishDate | 2024-04-01 |
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| spelling | doaj.art-e518b606c75743c590bc9e4b80b1644f2024-04-21T11:17:10ZengNature PortfolioScientific Reports2045-23222024-04-0114111210.1038/s41598-024-58725-0Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seedsZhaohui Ran0Zhi Li1Xu Xiao2Chao Yan3Mingtai An4Juyan Chen5Ming Tang6College of Forestry, Guizhou UniversityCollege of Forestry, Guizhou UniversityCollege of Forestry, Guizhou UniversityCollege of Forestry, Guizhou UniversityCollege of Forestry, Guizhou UniversityGuizhou Academy of Forestry SciencesCollege of Forestry, Jiangxi Agricultural UniversityAbstract Sect. tuberculata plant belongs to the Camellia genus and is named for the “tuberculiform protuberance on the surface of the ovary and fruit”. It is a species of great ornamental value and potential medicinal value. However, little has been reported on the metabolites of C. tuberculata seeds. Therefore, this study was conducted to investigate the metabolites of C. tuberculata seeds based on UPLC/ESI-Q TRAP-MS/MS with extensively targeted metabolomics. A total of 1611 metabolites were identified, including 107 alkaloids, 276 amino acids and derivatives, 283 flavonoids, 86 lignans and coumarins, 181 lipids, 68 nucleotides and derivatives, 101 organic acids, 190 phenolic acids, 10 quinones, 4 steroids, 17 tannins, 111 terpenoids, and 177 other metabolites. We compared the different metabolites in seeds between HKH, ZM, ZY, and LY. The 1311 identified different metabolites were classified into three categories. Sixty-three overlapping significant different metabolites were found, of which lignans and coumarins accounted for the largest proportion. The differentially accumulated metabolites were enriched in different metabolic pathways between HKH vs. LY, HKH vs. ZM, HKH vs. ZY, LY vs. ZY, ZM vs. LY and ZM vs. ZY, with the most abundant metabolic pathways being 4, 2, 4, 7, 7 and 5, respectively (p < 0.05). Moreover, among the top 20 metabolites in each subgroup comparison in terms of difference multiplicity 7, 8 and 13. ZM and ZY had the highest phenolic acid content. Ninety-six disease-resistant metabolites and 48 major traditional Chinese medicine agents were identified based on seven diseases. The results of this study will not only lead to a more comprehensive and in-depth understanding of the metabolic properties of C. tuberculata seeds, but also provide a scientific basis for the excavation and further development of its medicinal value.https://doi.org/10.1038/s41598-024-58725-0Camellia tuberculataUPLC/ESI-Q TRAP-MS/MSSeedsMetabolitesAntioxidant activityDisease resistance |
| spellingShingle | Zhaohui Ran Zhi Li Xu Xiao Chao Yan Mingtai An Juyan Chen Ming Tang Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seeds Scientific Reports Camellia tuberculata UPLC/ESI-Q TRAP-MS/MS Seeds Metabolites Antioxidant activity Disease resistance |
| title | Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seeds |
| title_full | Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seeds |
| title_fullStr | Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seeds |
| title_full_unstemmed | Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seeds |
| title_short | Extensive targeted metabolomics analysis reveals the identification of major metabolites, antioxidants, and disease-resistant active pharmaceutical components in Camellia tuberculata (Camellia L.) seeds |
| title_sort | extensive targeted metabolomics analysis reveals the identification of major metabolites antioxidants and disease resistant active pharmaceutical components in camellia tuberculata camellia l seeds |
| topic | Camellia tuberculata UPLC/ESI-Q TRAP-MS/MS Seeds Metabolites Antioxidant activity Disease resistance |
| url | https://doi.org/10.1038/s41598-024-58725-0 |
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