Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis
Abstract Background Propofol is a kind of common intravenous anaesthetic agent that plays an anti-tumor role in a variety of cancers, including ovarian cancer. However, the working mechanism of Propofol in ovarian cancer needs further exploration. Methods The viability and metastasis of ovarian canc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13048-021-00775-3 |
| _version_ | 1828080891108261888 |
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| author | Huan Lu Guanlin Zheng Xiang Gao Chanjuan Chen Min Zhou Longxin Zhang |
| author_facet | Huan Lu Guanlin Zheng Xiang Gao Chanjuan Chen Min Zhou Longxin Zhang |
| author_sort | Huan Lu |
| collection | DOAJ |
| description | Abstract Background Propofol is a kind of common intravenous anaesthetic agent that plays an anti-tumor role in a variety of cancers, including ovarian cancer. However, the working mechanism of Propofol in ovarian cancer needs further exploration. Methods The viability and metastasis of ovarian cancer cells were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and transwell assays. Flow cytometry was used to evaluate the cell cycle and apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine the abundance of circular RNA vacuolar protein sorting 13 homolog C (circVPS13C) and microRNA-145 (miR-145). The target relationship between miR-145 and circVPS13C was predicted by circinteractome database and verified by dual-luciferase reporter assay, RNA-binding protein immunoprecipitation (RIP) assay and RNA-pull down assay. Western blot assay was used to detect the levels of phosphorylated extracellular regulated MAP kinase (p-ERK), ERK, p-MAP kinse-ERK kinase (p-MEK) and MEK, in ovarian cancer cells. Results Propofol treatment suppressed the viability, cell cycle and motility and elevated the apoptosis rate of ovarian cancer cells. Propofol up-regulated miR-145 in a dose-dependent manner. Propofol exerted an anti-tumor role partly through up-regulating miR-145. MiR-145 was a direct target of circVPS13C. Propofol suppressed the progression of ovarian cancer through up-regulating miR-145 via suppressing circVPS13C. Propofol functioned through circVPS13C/miR-145/MEK/ERK signaling in ovarian cancer cells. Conclusion Propofol suppressed the proliferation, cell cycle, migration and invasion and induced the apoptosis of ovarian cancer cells through circVPS13C/miR-145/MEK/ERK signaling in vitro. |
| first_indexed | 2024-04-11T03:26:21Z |
| format | Article |
| id | doaj.art-e519fee8629142c2af8b3c0a3feae97e |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:26:21Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-e519fee8629142c2af8b3c0a3feae97e2023-01-02T07:39:49ZengBMCJournal of Ovarian Research1757-22152021-02-0114111110.1186/s13048-021-00775-3Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axisHuan Lu0Guanlin Zheng1Xiang Gao2Chanjuan Chen3Min Zhou4Longxin Zhang5Department of Anesthesiology, Fujian Provincial Maternity and Children’s Hospital, Affiliated Hospital of Fujian Medical UniversityDepartment of Anesthesiology, Fujian Provincial Maternity and Children’s Hospital, Affiliated Hospital of Fujian Medical UniversityDepartment of Anesthesiology, Fujian Provincial Maternity and Children’s Hospital, Affiliated Hospital of Fujian Medical UniversityDepartment of Anesthesiology, Fujian Provincial Maternity and Children’s Hospital, Affiliated Hospital of Fujian Medical UniversityDepartment of Anesthesiology, Fujian Provincial Maternity and Children’s Hospital, Affiliated Hospital of Fujian Medical UniversityDepartment of Anesthesiology, Fujian Provincial Maternity and Children’s Hospital, Affiliated Hospital of Fujian Medical UniversityAbstract Background Propofol is a kind of common intravenous anaesthetic agent that plays an anti-tumor role in a variety of cancers, including ovarian cancer. However, the working mechanism of Propofol in ovarian cancer needs further exploration. Methods The viability and metastasis of ovarian cancer cells were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and transwell assays. Flow cytometry was used to evaluate the cell cycle and apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine the abundance of circular RNA vacuolar protein sorting 13 homolog C (circVPS13C) and microRNA-145 (miR-145). The target relationship between miR-145 and circVPS13C was predicted by circinteractome database and verified by dual-luciferase reporter assay, RNA-binding protein immunoprecipitation (RIP) assay and RNA-pull down assay. Western blot assay was used to detect the levels of phosphorylated extracellular regulated MAP kinase (p-ERK), ERK, p-MAP kinse-ERK kinase (p-MEK) and MEK, in ovarian cancer cells. Results Propofol treatment suppressed the viability, cell cycle and motility and elevated the apoptosis rate of ovarian cancer cells. Propofol up-regulated miR-145 in a dose-dependent manner. Propofol exerted an anti-tumor role partly through up-regulating miR-145. MiR-145 was a direct target of circVPS13C. Propofol suppressed the progression of ovarian cancer through up-regulating miR-145 via suppressing circVPS13C. Propofol functioned through circVPS13C/miR-145/MEK/ERK signaling in ovarian cancer cells. Conclusion Propofol suppressed the proliferation, cell cycle, migration and invasion and induced the apoptosis of ovarian cancer cells through circVPS13C/miR-145/MEK/ERK signaling in vitro.https://doi.org/10.1186/s13048-021-00775-3Ovarian cancerPropofolcircVPS13CmiR-145MEK/ERK signaling |
| spellingShingle | Huan Lu Guanlin Zheng Xiang Gao Chanjuan Chen Min Zhou Longxin Zhang Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis Journal of Ovarian Research Ovarian cancer Propofol circVPS13C miR-145 MEK/ERK signaling |
| title | Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis |
| title_full | Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis |
| title_fullStr | Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis |
| title_full_unstemmed | Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis |
| title_short | Propofol suppresses cell viability, cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing MEK/ERK signaling via targeting circVPS13C/miR-145 axis |
| title_sort | propofol suppresses cell viability cell cycle progression and motility and induces cell apoptosis of ovarian cancer cells through suppressing mek erk signaling via targeting circvps13c mir 145 axis |
| topic | Ovarian cancer Propofol circVPS13C miR-145 MEK/ERK signaling |
| url | https://doi.org/10.1186/s13048-021-00775-3 |
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