<i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and...

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Main Authors: Bárbara Ribeiro Alves Alencar, Renan Anderson Alves de Freitas, Victor Emanuel Petrício Guimarães, Rayssa Karla Silva, Carolina Elsztein, Suzyanne Porfírio da Silva, Emmanuel Damilano Dutra, Marcos Antonio de Morais Junior, Rafael Barros de Souza
Format: Article
Language:English
Published: MDPI AG 2023-07-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/9/8/789
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author Bárbara Ribeiro Alves Alencar
Renan Anderson Alves de Freitas
Victor Emanuel Petrício Guimarães
Rayssa Karla Silva
Carolina Elsztein
Suzyanne Porfírio da Silva
Emmanuel Damilano Dutra
Marcos Antonio de Morais Junior
Rafael Barros de Souza
author_facet Bárbara Ribeiro Alves Alencar
Renan Anderson Alves de Freitas
Victor Emanuel Petrício Guimarães
Rayssa Karla Silva
Carolina Elsztein
Suzyanne Porfírio da Silva
Emmanuel Damilano Dutra
Marcos Antonio de Morais Junior
Rafael Barros de Souza
author_sort Bárbara Ribeiro Alves Alencar
collection DOAJ
description The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of <i>Meyerozyma caribbica</i> URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose was present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.
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spelling doaj.art-e51b83a62f5649b997ce5c4c582dedf92023-11-19T01:47:02ZengMDPI AGJournal of Fungi2309-608X2023-07-019878910.3390/jof9080789<i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in BiorefineriesBárbara Ribeiro Alves Alencar0Renan Anderson Alves de Freitas1Victor Emanuel Petrício Guimarães2Rayssa Karla Silva3Carolina Elsztein4Suzyanne Porfírio da Silva5Emmanuel Damilano Dutra6Marcos Antonio de Morais Junior7Rafael Barros de Souza8Laboratory of Biomass Energy, Department of Nuclear Energy, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Microbial Metabolism, Institute of Biological Sciences, University of Pernambuco, Recife 50110-000, BrazilLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Biomass Energy, Department of Nuclear Energy, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Biomass Energy, Department of Nuclear Energy, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife 50670-901, BrazilLaboratory of Microbial Metabolism, Institute of Biological Sciences, University of Pernambuco, Recife 50110-000, BrazilThe production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of <i>Meyerozyma caribbica</i> URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose was present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.https://www.mdpi.com/2309-608X/9/8/789cactushydrolysatelignocellulosemixed substratenon-conventional yeast
spellingShingle Bárbara Ribeiro Alves Alencar
Renan Anderson Alves de Freitas
Victor Emanuel Petrício Guimarães
Rayssa Karla Silva
Carolina Elsztein
Suzyanne Porfírio da Silva
Emmanuel Damilano Dutra
Marcos Antonio de Morais Junior
Rafael Barros de Souza
<i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries
Journal of Fungi
cactus
hydrolysate
lignocellulose
mixed substrate
non-conventional yeast
title <i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries
title_full <i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries
title_fullStr <i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries
title_full_unstemmed <i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries
title_short <i>Meyerozyma caribbica</i> Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries
title_sort i meyerozyma caribbica i isolated from vinasse irrigated sugarcane plantation soil a promising yeast for ethanol and xylitol production in biorefineries
topic cactus
hydrolysate
lignocellulose
mixed substrate
non-conventional yeast
url https://www.mdpi.com/2309-608X/9/8/789
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