Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral Islands
Heat shock protein 20 (Hsp20) is a major family of heat shock proteins that mainly function as molecular chaperones and are markedly accumulated in cells when organisms are subjected to environmental stress, particularly heat. <i>Canavalia rosea</i> is an extremophile halophyte with good...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6405 |
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| author | Mei Zhang Shuguang Jian Zhengfeng Wang |
| author_facet | Mei Zhang Shuguang Jian Zhengfeng Wang |
| author_sort | Mei Zhang |
| collection | DOAJ |
| description | Heat shock protein 20 (Hsp20) is a major family of heat shock proteins that mainly function as molecular chaperones and are markedly accumulated in cells when organisms are subjected to environmental stress, particularly heat. <i>Canavalia rosea</i> is an extremophile halophyte with good adaptability to environmental high temperature and is widely distributed in coastal areas or islands in tropical and subtropical regions. In this study, we identified a total of 41 <i>CrHsp20</i> genes in the <i>C. rosea</i> genome. The gene structures, phylogenetic relationships, chromosome locations, and conserved motifs of each <i>CrHsp20</i> or encoding protein were analyzed. The promoters of <i>CrHsp20</i>s contained a series of predicted <i>cis</i>-acting elements, which indicates that the expression of different <i>CrHsp20</i> members is regulated precisely. The expression patterns of the <i>CrHsp20</i> family were analyzed by RNA sequencing both at the tissue-specific level and under different abiotic stresses, and were further validated by quantitative reverse transcription PCR. The integrated expression profiles of the <i>CrHsp20</i>s indicated that most <i>CrHsp20</i> genes were greatly upregulated (up to dozens to thousands of times) after 2 h of heat stress. However, some of the heat-upregulated <i>CrHsp20</i> genes showed completely different expression patterns in response to salt, alkaline, or high osmotic stresses, which indicates their potential specific function in mediating the response of <i>C. rosea</i> to abiotic stresses. In addition, some of <i>CrHsp20</i>s were cloned and functionally characterized for their roles in abiotic stress tolerance in yeast. Taken together, these findings provide a foundation for functionally characterizing <i>Hsp20</i>s to unravel their possible roles in the adaptation of this species to tropical coral reefs. Our results also contribute to the understanding of the complexity of the response of <i>CrHsp20</i> genes to other abiotic stresses and may help in future studies evaluating the functional characteristics of <i>CrHsp20</i>s for crop genetic improvement. |
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| spelling | doaj.art-e51f272c391b44e9b2fdb3ee9d68fbaf2023-11-23T17:00:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312640510.3390/ijms23126405Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral IslandsMei Zhang0Shuguang Jian1Zhengfeng Wang2Guangdong Provincial Key Laboratory of Applied Botany and South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaCAS Engineering Laboratory for Vegetation Ecosystem Restoration on Islands and Coastal Zones, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaGuangdong Provincial Key Laboratory of Applied Botany and South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaHeat shock protein 20 (Hsp20) is a major family of heat shock proteins that mainly function as molecular chaperones and are markedly accumulated in cells when organisms are subjected to environmental stress, particularly heat. <i>Canavalia rosea</i> is an extremophile halophyte with good adaptability to environmental high temperature and is widely distributed in coastal areas or islands in tropical and subtropical regions. In this study, we identified a total of 41 <i>CrHsp20</i> genes in the <i>C. rosea</i> genome. The gene structures, phylogenetic relationships, chromosome locations, and conserved motifs of each <i>CrHsp20</i> or encoding protein were analyzed. The promoters of <i>CrHsp20</i>s contained a series of predicted <i>cis</i>-acting elements, which indicates that the expression of different <i>CrHsp20</i> members is regulated precisely. The expression patterns of the <i>CrHsp20</i> family were analyzed by RNA sequencing both at the tissue-specific level and under different abiotic stresses, and were further validated by quantitative reverse transcription PCR. The integrated expression profiles of the <i>CrHsp20</i>s indicated that most <i>CrHsp20</i> genes were greatly upregulated (up to dozens to thousands of times) after 2 h of heat stress. However, some of the heat-upregulated <i>CrHsp20</i> genes showed completely different expression patterns in response to salt, alkaline, or high osmotic stresses, which indicates their potential specific function in mediating the response of <i>C. rosea</i> to abiotic stresses. In addition, some of <i>CrHsp20</i>s were cloned and functionally characterized for their roles in abiotic stress tolerance in yeast. Taken together, these findings provide a foundation for functionally characterizing <i>Hsp20</i>s to unravel their possible roles in the adaptation of this species to tropical coral reefs. Our results also contribute to the understanding of the complexity of the response of <i>CrHsp20</i> genes to other abiotic stresses and may help in future studies evaluating the functional characteristics of <i>CrHsp20</i>s for crop genetic improvement.https://www.mdpi.com/1422-0067/23/12/6405abiotic stress<i>Canavalia rosea</i>gene expressionheat shock protein 20 |
| spellingShingle | Mei Zhang Shuguang Jian Zhengfeng Wang Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral Islands International Journal of Molecular Sciences abiotic stress <i>Canavalia rosea</i> gene expression heat shock protein 20 |
| title | Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral Islands |
| title_full | Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral Islands |
| title_fullStr | Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral Islands |
| title_full_unstemmed | Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral Islands |
| title_short | Comprehensive Analysis of the <i>Hsp20</i> Gene Family in <i>Canavalia rosea</i> Indicates Its Roles in the Response to Multiple Abiotic Stresses and Adaptation to Tropical Coral Islands |
| title_sort | comprehensive analysis of the i hsp20 i gene family in i canavalia rosea i indicates its roles in the response to multiple abiotic stresses and adaptation to tropical coral islands |
| topic | abiotic stress <i>Canavalia rosea</i> gene expression heat shock protein 20 |
| url | https://www.mdpi.com/1422-0067/23/12/6405 |
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