Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)
Abstract Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil’s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytoc...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-10-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100285&tlng=en |
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| author | T. S. Neri K. W. L. Silva L. P. S. Maior S. K. Oliveira-Silva P. V. M. Azevedo D. C. S. Gomes M. A. Souza J. M. S. J. Pavão J. G. Costa A. L. Cunha G. C. Ferreira-Júnior T. J. Matos-Rocha A. F. Santos S. A. Fonseca |
| author_facet | T. S. Neri K. W. L. Silva L. P. S. Maior S. K. Oliveira-Silva P. V. M. Azevedo D. C. S. Gomes M. A. Souza J. M. S. J. Pavão J. G. Costa A. L. Cunha G. C. Ferreira-Júnior T. J. Matos-Rocha A. F. Santos S. A. Fonseca |
| author_sort | T. S. Neri |
| collection | DOAJ |
| description | Abstract Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil’s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923. |
| first_indexed | 2024-04-11T15:19:50Z |
| format | Article |
| id | doaj.art-e51f2bfb5c504c52976b2138ac39b128 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-11T15:19:50Z |
| publishDate | 2021-10-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-e51f2bfb5c504c52976b2138ac39b1282022-12-22T04:16:24ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-10-018310.1590/1519-6984.236649Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)T. S. Nerihttps://orcid.org/0000-0002-0947-3619K. W. L. Silvahttps://orcid.org/0000-0001-7015-2489L. P. S. Maiorhttps://orcid.org/0000-0001-5322-0275S. K. Oliveira-Silvahttps://orcid.org/0000-0002-8447-6326P. V. M. Azevedohttps://orcid.org/0000-0002-6974-9410D. C. S. Gomeshttps://orcid.org/0000-0003-0079-793XM. A. Souzahttps://orcid.org/0000-0003-0823-1957J. M. S. J. Pavãohttps://orcid.org/0000-0002-5217-3857J. G. Costahttps://orcid.org/0000-0002-0761-0755A. L. Cunhahttps://orcid.org/0000-0002-2688-5025G. C. Ferreira-Júniorhttps://orcid.org/0000-0002-7359-3738T. J. Matos-Rochahttps://orcid.org/0000-0001-5153-6583A. F. Santoshttps://orcid.org/0000-0001-6049-9446S. A. Fonsecahttps://orcid.org/0000-0003-2091-7951Abstract Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil’s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100285&tlng=enCroton argyrophylloides Muell. Argantioxidantphytochemistryminimal inhibitory concentration |
| spellingShingle | T. S. Neri K. W. L. Silva L. P. S. Maior S. K. Oliveira-Silva P. V. M. Azevedo D. C. S. Gomes M. A. Souza J. M. S. J. Pavão J. G. Costa A. L. Cunha G. C. Ferreira-Júnior T. J. Matos-Rocha A. F. Santos S. A. Fonseca Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae) Brazilian Journal of Biology Croton argyrophylloides Muell. Arg antioxidant phytochemistry minimal inhibitory concentration |
| title | Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae) |
| title_full | Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae) |
| title_fullStr | Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae) |
| title_full_unstemmed | Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae) |
| title_short | Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae) |
| title_sort | phytochemical characterization antioxidant potential and antibacterial activity of the croton argyrophylloides muell arg euphorbiaceae |
| topic | Croton argyrophylloides Muell. Arg antioxidant phytochemistry minimal inhibitory concentration |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100285&tlng=en |
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