Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix?
Background: Cervical cancer is the fourth most common malignancy and fourth leading cause of cancer death in women worldwide. More than 99.7% of cases are caused by human papillomavirus (HPV), while HPV types 16 and 18 cause over 70% of all cervical cancer cases. In this preliminary study, we aimed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/10/2130 |
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| author | Tomas Rokos Veronika Holubekova Zuzana Kolkova Andrea Hornakova Terezia Pribulova Erik Kozubik Kamil Biringer Erik Kudela |
| author_facet | Tomas Rokos Veronika Holubekova Zuzana Kolkova Andrea Hornakova Terezia Pribulova Erik Kozubik Kamil Biringer Erik Kudela |
| author_sort | Tomas Rokos |
| collection | DOAJ |
| description | Background: Cervical cancer is the fourth most common malignancy and fourth leading cause of cancer death in women worldwide. More than 99.7% of cases are caused by human papillomavirus (HPV), while HPV types 16 and 18 cause over 70% of all cervical cancer cases. In this preliminary study, we aimed to investigate the presence of HPV infection and diversity of bacteria associated with bacterial vaginosis. Methods: Cervical swabs (<i>n</i> = 21) taken from women aged 21–47 years, in seventeen cases, with different degrees of cervical abnormality, and from four healthy women, were tested for the presence of HPV DNA, as well as the bacterial strains associated with bacterial vaginosis, using the real-time PCR method. Results: HPV16 was the dominant genotype in 53% (9/17) of patients with confirmed precancerous lesions (ASCUS, LSIL, and HSIL). In specimens with confirmed cytological abnormalities and hrHPV infection, we detected a wide diversity of microbes, while the most common species were <i>Gardnerella vaginalis</i>, <i>Atopobium vaginae</i>, <i>Prevotella bivia</i>, <i>Ureaplasma parvum</i>, <i>Ureaplasma urealyticum</i>, <i>Leptotrichia amnionii</i>, <i>Bacteroides ureolyticus</i>, and <i>Sneathia sanguinegens</i>. The presence of pathogens did not differ, depending on the degree of precancerous lesions or HPV type. Conclusion: In our work, HPV16 dominated in patients with cervical precancerous lesions. We also suggest an increased bacterial diversity of the vaginal microbiome in patients with cervical lesions, for which the HPV virus is largely responsible. |
| first_indexed | 2024-03-09T19:24:25Z |
| format | Article |
| id | doaj.art-e521df735deb44dc84ec7e5db7b2dc51 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T19:24:25Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e521df735deb44dc84ec7e5db7b2dc512023-11-24T03:07:56ZengMDPI AGViruses1999-49152022-09-011410213010.3390/v14102130Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix?Tomas Rokos0Veronika Holubekova1Zuzana Kolkova2Andrea Hornakova3Terezia Pribulova4Erik Kozubik5Kamil Biringer6Erik Kudela7Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2, 03601 Martin, SlovakiaDepartment of Molecular Oncology and Diagnostics, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University in Bratislava, Mala Hora 4C, 03601 Martin, SlovakiaDepartment of Molecular Oncology and Diagnostics, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University in Bratislava, Mala Hora 4C, 03601 Martin, SlovakiaDepartment of Molecular Oncology and Diagnostics, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University in Bratislava, Mala Hora 4C, 03601 Martin, SlovakiaDepartment of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2, 03601 Martin, SlovakiaDepartment of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2, 03601 Martin, SlovakiaDepartment of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2, 03601 Martin, SlovakiaDepartment of Gynecology and Obstetrics, Jessenius Faculty of Medicine, Comenius University in Bratislava, Kollarova 2, 03601 Martin, SlovakiaBackground: Cervical cancer is the fourth most common malignancy and fourth leading cause of cancer death in women worldwide. More than 99.7% of cases are caused by human papillomavirus (HPV), while HPV types 16 and 18 cause over 70% of all cervical cancer cases. In this preliminary study, we aimed to investigate the presence of HPV infection and diversity of bacteria associated with bacterial vaginosis. Methods: Cervical swabs (<i>n</i> = 21) taken from women aged 21–47 years, in seventeen cases, with different degrees of cervical abnormality, and from four healthy women, were tested for the presence of HPV DNA, as well as the bacterial strains associated with bacterial vaginosis, using the real-time PCR method. Results: HPV16 was the dominant genotype in 53% (9/17) of patients with confirmed precancerous lesions (ASCUS, LSIL, and HSIL). In specimens with confirmed cytological abnormalities and hrHPV infection, we detected a wide diversity of microbes, while the most common species were <i>Gardnerella vaginalis</i>, <i>Atopobium vaginae</i>, <i>Prevotella bivia</i>, <i>Ureaplasma parvum</i>, <i>Ureaplasma urealyticum</i>, <i>Leptotrichia amnionii</i>, <i>Bacteroides ureolyticus</i>, and <i>Sneathia sanguinegens</i>. The presence of pathogens did not differ, depending on the degree of precancerous lesions or HPV type. Conclusion: In our work, HPV16 dominated in patients with cervical precancerous lesions. We also suggest an increased bacterial diversity of the vaginal microbiome in patients with cervical lesions, for which the HPV virus is largely responsible.https://www.mdpi.com/1999-4915/14/10/2130human virusesHPVvaginal microbiomecervical precancerosis |
| spellingShingle | Tomas Rokos Veronika Holubekova Zuzana Kolkova Andrea Hornakova Terezia Pribulova Erik Kozubik Kamil Biringer Erik Kudela Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix? Viruses human viruses HPV vaginal microbiome cervical precancerosis |
| title | Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix? |
| title_full | Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix? |
| title_fullStr | Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix? |
| title_full_unstemmed | Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix? |
| title_short | Is the Physiological Composition of the Vaginal Microbiome Altered in High-Risk HPV Infection of the Uterine Cervix? |
| title_sort | is the physiological composition of the vaginal microbiome altered in high risk hpv infection of the uterine cervix |
| topic | human viruses HPV vaginal microbiome cervical precancerosis |
| url | https://www.mdpi.com/1999-4915/14/10/2130 |
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