Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae)
Seven geographic isolates of Helicoverpa armigera NPV were characterized by PCR-RFLP techniques and their comparative virulence to second instar larvae of H. armigera (Hübner) were compared before and after exposure to simulated sunlight as well as open weather conditions on cotton and tomato plant...
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| Format: | Article |
| Language: | English |
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Entomological Society of Iran
2008-08-01
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| Series: | نامه انجمن حشرهشناسی ایران |
| Subjects: | |
| Online Access: | https://jesi.areeo.ac.ir/article_105302_d91647fa691324a8e4e4e60fc43bec73.pdf |
| _version_ | 1797973248635305984 |
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| author | K. Veenakumari G. B. Narabenchi R. J. Rabindra A. Mehrvar |
| author_facet | K. Veenakumari G. B. Narabenchi R. J. Rabindra A. Mehrvar |
| author_sort | K. Veenakumari |
| collection | DOAJ |
| description | Seven geographic isolates of Helicoverpa armigera NPV were characterized by PCR-RFLP techniques and their comparative virulence to second instar larvae of H. armigera (Hübner) were compared before and after exposure to simulated sunlight as well as open weather conditions on cotton and tomato plants. The PCR products of 25K FP region showed no difference among the isolates (780 bp). The PCR amplification products revealed genetic diversity among the 7 HearNPV isolates when they were digested individually using four different restriction enzymes (HindIII, BamHI, PstI, and EcoRI). The bioassays of the HearNPV isolates against the second instar larvae of H. armigera, before and after exposure to simulated sunlight, revealed a range of variation in their biological activity. However, the NGM isolate was the most virulent with the lowest LC50 and LT50 values. The dose-inhibitory response of the second instar larvae of H. armigera to the virus isolates revealed NGM isolate to be the least susceptible to simulated sunlight, having the highest ID50 value (462.9 W/m2). Experiments on persistence of HearNPV isolates on cotton and tomato plants under open weather conditions showed that the persistence of the viral isolates was relatively higher on tomato than on cotton. |
| first_indexed | 2024-04-11T04:01:15Z |
| format | Article |
| id | doaj.art-e52e45d39c264507b289bf07e2edc6a2 |
| institution | Directory Open Access Journal |
| issn | 0259-9996 2783-3968 |
| language | English |
| last_indexed | 2024-04-11T04:01:15Z |
| publishDate | 2008-08-01 |
| publisher | Entomological Society of Iran |
| record_format | Article |
| series | نامه انجمن حشرهشناسی ایران |
| spelling | doaj.art-e52e45d39c264507b289bf07e2edc6a22023-01-01T23:33:17ZengEntomological Society of Iranنامه انجمن حشرهشناسی ایران0259-99962783-39682008-08-012813960105302Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae)K. VeenakumariG. B. NarabenchiR. J. RabindraA. MehrvarSeven geographic isolates of Helicoverpa armigera NPV were characterized by PCR-RFLP techniques and their comparative virulence to second instar larvae of H. armigera (Hübner) were compared before and after exposure to simulated sunlight as well as open weather conditions on cotton and tomato plants. The PCR products of 25K FP region showed no difference among the isolates (780 bp). The PCR amplification products revealed genetic diversity among the 7 HearNPV isolates when they were digested individually using four different restriction enzymes (HindIII, BamHI, PstI, and EcoRI). The bioassays of the HearNPV isolates against the second instar larvae of H. armigera, before and after exposure to simulated sunlight, revealed a range of variation in their biological activity. However, the NGM isolate was the most virulent with the lowest LC50 and LT50 values. The dose-inhibitory response of the second instar larvae of H. armigera to the virus isolates revealed NGM isolate to be the least susceptible to simulated sunlight, having the highest ID50 value (462.9 W/m2). Experiments on persistence of HearNPV isolates on cotton and tomato plants under open weather conditions showed that the persistence of the viral isolates was relatively higher on tomato than on cotton.https://jesi.areeo.ac.ir/article_105302_d91647fa691324a8e4e4e60fc43bec73.pdfnucleopolyhedrovirushelicoverpa armigerageographic isolates25k fp genebiological activitysimulated sunlighthost plant |
| spellingShingle | K. Veenakumari G. B. Narabenchi R. J. Rabindra A. Mehrvar Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae) نامه انجمن حشرهشناسی ایران nucleopolyhedrovirus helicoverpa armigera geographic isolates 25k fp gene biological activity simulated sunlight host plant |
| title | Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae) |
| title_full | Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae) |
| title_fullStr | Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae) |
| title_full_unstemmed | Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae) |
| title_short | Molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae) |
| title_sort | molecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of helicoverpa armigera lep noctuidae |
| topic | nucleopolyhedrovirus helicoverpa armigera geographic isolates 25k fp gene biological activity simulated sunlight host plant |
| url | https://jesi.areeo.ac.ir/article_105302_d91647fa691324a8e4e4e60fc43bec73.pdf |
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