KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum
ABSTRACTNapier grass and pearl millet are tropical forages from the genus Pennisetum. The variability in those species is explored in breeding programs of forages, as well as in the production of interspecific hybrids. Hybridization is a phenomenon that leads to intergenomic conflicts following the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Lavras
2015-10-01
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| Series: | Ciência e Agrotecnologia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500443&lng=en&tlng=en |
| _version_ | 1819097498395869184 |
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| author | Gabriela Barreto dos Reis Larissa Fonseca Andrade-Vieira José Marcello Salabert de Campos Antonio Vander Pereira Lisete Chamma Davide |
| author_facet | Gabriela Barreto dos Reis Larissa Fonseca Andrade-Vieira José Marcello Salabert de Campos Antonio Vander Pereira Lisete Chamma Davide |
| author_sort | Gabriela Barreto dos Reis |
| collection | DOAJ |
| description | ABSTRACTNapier grass and pearl millet are tropical forages from the genus Pennisetum. The variability in those species is explored in breeding programs of forages, as well as in the production of interspecific hybrids. Hybridization is a phenomenon that leads to intergenomic conflicts following the elimination of genomic sequences. In this sense, the present work aimed to study the genomic alterations occurring after interspecific hybridization of pearl millet and Napier grass with the use of cytogenetics and flow cytometry tools. These methods allowed the evaluation of chromosome morphometry, DNA content and genomic ratio in pearl millet, Napier grass and hybrids. It was observed that pearl millet and Napier grass have chromosomes with superposed size. The hybrid presents chromosomes that are smaller than expected, leading to karyotype alterations. Additionally, comparing the DNA content of parents and hybrids, loss of DNA content was demonstrated. Further, changes in the pearl millet and Napier grass genome ratio were also verified in the hybrid nucleus. Moreover, genomic rearrangements were shown to occur through karyotype alterations in the hybrid. |
| first_indexed | 2024-12-22T00:16:03Z |
| format | Article |
| id | doaj.art-e52f0a7bde3347a9b18a87dbda471c72 |
| institution | Directory Open Access Journal |
| issn | 1981-1829 |
| language | English |
| last_indexed | 2024-12-22T00:16:03Z |
| publishDate | 2015-10-01 |
| publisher | Universidade Federal de Lavras |
| record_format | Article |
| series | Ciência e Agrotecnologia |
| spelling | doaj.art-e52f0a7bde3347a9b18a87dbda471c722022-12-21T18:45:18ZengUniversidade Federal de LavrasCiência e Agrotecnologia1981-18292015-10-0139544345410.1590/S1413-70542015000500003S1413-70542015000500443KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucumGabriela Barreto dos ReisLarissa Fonseca Andrade-VieiraJosé Marcello Salabert de CamposAntonio Vander PereiraLisete Chamma DavideABSTRACTNapier grass and pearl millet are tropical forages from the genus Pennisetum. The variability in those species is explored in breeding programs of forages, as well as in the production of interspecific hybrids. Hybridization is a phenomenon that leads to intergenomic conflicts following the elimination of genomic sequences. In this sense, the present work aimed to study the genomic alterations occurring after interspecific hybridization of pearl millet and Napier grass with the use of cytogenetics and flow cytometry tools. These methods allowed the evaluation of chromosome morphometry, DNA content and genomic ratio in pearl millet, Napier grass and hybrids. It was observed that pearl millet and Napier grass have chromosomes with superposed size. The hybrid presents chromosomes that are smaller than expected, leading to karyotype alterations. Additionally, comparing the DNA content of parents and hybrids, loss of DNA content was demonstrated. Further, changes in the pearl millet and Napier grass genome ratio were also verified in the hybrid nucleus. Moreover, genomic rearrangements were shown to occur through karyotype alterations in the hybrid.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500443&lng=en&tlng=enHíbridos interespecíficosmorfometria cromossômicaconteúdo de DNAhibridização in situ |
| spellingShingle | Gabriela Barreto dos Reis Larissa Fonseca Andrade-Vieira José Marcello Salabert de Campos Antonio Vander Pereira Lisete Chamma Davide KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum Ciência e Agrotecnologia Híbridos interespecíficos morfometria cromossômica conteúdo de DNA hibridização in situ |
| title | KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum |
| title_full | KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum |
| title_fullStr | KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum |
| title_full_unstemmed | KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum |
| title_short | KARYOTYPE ALTERATIONS AFTER HYBRIDIZATION BETWEEN Pennisetum purpureum AND Pennisetum glaucum |
| title_sort | karyotype alterations after hybridization between pennisetum purpureum and pennisetum glaucum |
| topic | Híbridos interespecíficos morfometria cromossômica conteúdo de DNA hibridização in situ |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500443&lng=en&tlng=en |
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