Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells
Abstract Aim The purpose of this study was to determine the impact of Elaeagnus Angustifolia extract (EA) on human dermal fibroblast (HDF) survival, migration, and wound healing-related genes. Methods After preparing the hydroalcoholic extract of EA, MTT and scratch tests were used to determine the...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-023-06644-0 |
| _version_ | 1827590807121559552 |
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| author | Ehsaneh Azaryan Sepideh Sarfi Seyede Fatemeh Hosseini Mansoore Saharkhiz Khadijeh vazifeshenas-Darmiyan Mohsen Naseri |
| author_facet | Ehsaneh Azaryan Sepideh Sarfi Seyede Fatemeh Hosseini Mansoore Saharkhiz Khadijeh vazifeshenas-Darmiyan Mohsen Naseri |
| author_sort | Ehsaneh Azaryan |
| collection | DOAJ |
| description | Abstract Aim The purpose of this study was to determine the impact of Elaeagnus Angustifolia extract (EA) on human dermal fibroblast (HDF) survival, migration, and wound healing-related genes. Methods After preparing the hydroalcoholic extract of EA, MTT and scratch tests were used to determine the effect of EA on the viability and migration of HDFs. In addition, the quantitative polymerase chain reaction (q-PCR) was conducted to evaluate the impact of EA on the expression of wound healing-related genes in HDFs. Result According to the MTT test, a nontoxic concentration of EA (100 µg/ml) was obtained for further investigations. The scratch test results demonstrated that EA improved HDFs’ capacity to migrate when compared to the control group. Additionally, q-PCR results revealed that EA could significantly increase wound healing-related genes (VEGF-A, HLA-G5, and IL-6) in comparison with the control group. Conclusions The EA could have a significant impact on the viability and migration of HDFs. Also, EA increased the expression of wound healing-related genes. |
| first_indexed | 2024-03-09T01:21:57Z |
| format | Article |
| id | doaj.art-e5309089e57340a19c9cdbcc340eab86 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-09T01:21:57Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-e5309089e57340a19c9cdbcc340eab862023-12-10T12:06:04ZengBMCBMC Research Notes1756-05002023-12-011611610.1186/s13104-023-06644-0Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cellsEhsaneh Azaryan0Sepideh Sarfi1Seyede Fatemeh Hosseini2Mansoore Saharkhiz3Khadijeh vazifeshenas-Darmiyan4Mohsen Naseri5Cellular and Molecular Research Center, Department of Molecular Medicine, Birjand University of Medical SciencesStudent Research Committee, Birjand University of Medical SciencesDepartment of Anatomy, Tabas School of Nursing, Birjand University of Medical sciencesDepartment of Immunology, Faculty of Medicine, Birjand University of Medical SciencesPhD student in Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical SciencesCellular and Molecular Research Center, Department of Molecular Medicine, Birjand University of Medical SciencesAbstract Aim The purpose of this study was to determine the impact of Elaeagnus Angustifolia extract (EA) on human dermal fibroblast (HDF) survival, migration, and wound healing-related genes. Methods After preparing the hydroalcoholic extract of EA, MTT and scratch tests were used to determine the effect of EA on the viability and migration of HDFs. In addition, the quantitative polymerase chain reaction (q-PCR) was conducted to evaluate the impact of EA on the expression of wound healing-related genes in HDFs. Result According to the MTT test, a nontoxic concentration of EA (100 µg/ml) was obtained for further investigations. The scratch test results demonstrated that EA improved HDFs’ capacity to migrate when compared to the control group. Additionally, q-PCR results revealed that EA could significantly increase wound healing-related genes (VEGF-A, HLA-G5, and IL-6) in comparison with the control group. Conclusions The EA could have a significant impact on the viability and migration of HDFs. Also, EA increased the expression of wound healing-related genes.https://doi.org/10.1186/s13104-023-06644-0Elaeagnus AngustifoliaHuman dermal fibroblastWound healing |
| spellingShingle | Ehsaneh Azaryan Sepideh Sarfi Seyede Fatemeh Hosseini Mansoore Saharkhiz Khadijeh vazifeshenas-Darmiyan Mohsen Naseri Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells BMC Research Notes Elaeagnus Angustifolia Human dermal fibroblast Wound healing |
| title | Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells |
| title_full | Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells |
| title_fullStr | Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells |
| title_full_unstemmed | Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells |
| title_short | Effect of Elaeagnus Angustifolia extract on in vitro wound healing of human dermal fibroblast cells |
| title_sort | effect of elaeagnus angustifolia extract on in vitro wound healing of human dermal fibroblast cells |
| topic | Elaeagnus Angustifolia Human dermal fibroblast Wound healing |
| url | https://doi.org/10.1186/s13104-023-06644-0 |
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