Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle
Extracts from <i>Drosera rotundifolia</i> are traditionally used to treat cough symptoms during a common cold. The present study aimed to investigate the impact of extracts from <i>D. rotundifolia</i> and active compounds on the respiratory tract. Tracheal slices of C57BL/6N...
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6622 |
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| author | Alexander Hake Frank Begrow Verena Spiegler Nico Symma Andreas Hensel Martina Düfer |
| author_facet | Alexander Hake Frank Begrow Verena Spiegler Nico Symma Andreas Hensel Martina Düfer |
| author_sort | Alexander Hake |
| collection | DOAJ |
| description | Extracts from <i>Drosera rotundifolia</i> are traditionally used to treat cough symptoms during a common cold. The present study aimed to investigate the impact of extracts from <i>D. rotundifolia</i> and active compounds on the respiratory tract. Tracheal slices of C57BL/6N mice were used ex vivo to examine effects on airway smooth muscle (ASM) and ciliary beat frequency (CBF). Phosphodiesterase (PDE) inhibition assays were carried out to test whether PDE1 or PDE4 are targeted by the active compounds. An ethanol–water extract, as well as an aqueous fraction of this extract, exerted antispasmodic properties against acetylcholine-induced contractions. In addition, contractions induced by 60 mM K<sup>+</sup> were abrogated by the aqueous fraction. Effects on ASM could be attributed to the flavonoids quercetin, 2″-<i>O</i>-galloylhyperoside and hyperoside. Moreover, the <i>Drosera</i> extract and the aqueous fraction increased the CBF of murine tracheal slices. Quercetin and 2″-<i>O</i>-galloylhyperoside were identified as active compounds involved in the elevation of CBF. Both compounds inhibited PDE1A and PDE4D. The elevation of CBF was mimicked by the subtype-selective PDE inhibitor rolipram (PDE4) and by 8-methoxymethyl-IBMX. In summary, our study shows, for the first time, that a <i>Drosera</i> extract and its flavonoid compounds increase the CBF of murine airways while antispasmodic effects were transferred to ASM. |
| first_indexed | 2024-03-09T21:24:10Z |
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| language | English |
| last_indexed | 2024-03-09T21:24:10Z |
| publishDate | 2022-10-01 |
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| series | Molecules |
| spelling | doaj.art-e5345eeb58d444cfad8749919f1ea3912023-11-23T21:14:28ZengMDPI AGMolecules1420-30492022-10-012719662210.3390/molecules27196622Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth MuscleAlexander Hake0Frank Begrow1Verena Spiegler2Nico Symma3Andreas Hensel4Martina Düfer5Institute of Pharmaceutical and Medicinal Chemistry—Pharmacology, University of Münster, 48149 Münster, GermanyInstitute of Pharmaceutical and Medicinal Chemistry—Pharmacology, University of Münster, 48149 Münster, GermanyInstitute of Pharmaceutical Biology and Phytochemistry, University of Münster, 48149 Münster, GermanyInstitute of Pharmaceutical Biology and Phytochemistry, University of Münster, 48149 Münster, GermanyInstitute of Pharmaceutical Biology and Phytochemistry, University of Münster, 48149 Münster, GermanyInstitute of Pharmaceutical and Medicinal Chemistry—Pharmacology, University of Münster, 48149 Münster, GermanyExtracts from <i>Drosera rotundifolia</i> are traditionally used to treat cough symptoms during a common cold. The present study aimed to investigate the impact of extracts from <i>D. rotundifolia</i> and active compounds on the respiratory tract. Tracheal slices of C57BL/6N mice were used ex vivo to examine effects on airway smooth muscle (ASM) and ciliary beat frequency (CBF). Phosphodiesterase (PDE) inhibition assays were carried out to test whether PDE1 or PDE4 are targeted by the active compounds. An ethanol–water extract, as well as an aqueous fraction of this extract, exerted antispasmodic properties against acetylcholine-induced contractions. In addition, contractions induced by 60 mM K<sup>+</sup> were abrogated by the aqueous fraction. Effects on ASM could be attributed to the flavonoids quercetin, 2″-<i>O</i>-galloylhyperoside and hyperoside. Moreover, the <i>Drosera</i> extract and the aqueous fraction increased the CBF of murine tracheal slices. Quercetin and 2″-<i>O</i>-galloylhyperoside were identified as active compounds involved in the elevation of CBF. Both compounds inhibited PDE1A and PDE4D. The elevation of CBF was mimicked by the subtype-selective PDE inhibitor rolipram (PDE4) and by 8-methoxymethyl-IBMX. In summary, our study shows, for the first time, that a <i>Drosera</i> extract and its flavonoid compounds increase the CBF of murine airways while antispasmodic effects were transferred to ASM.https://www.mdpi.com/1420-3049/27/19/6622<i>Drosera rotundifolia</i>antispasmodictracheaciliary beat frequencyphosphodiesterase |
| spellingShingle | Alexander Hake Frank Begrow Verena Spiegler Nico Symma Andreas Hensel Martina Düfer Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle Molecules <i>Drosera rotundifolia</i> antispasmodic trachea ciliary beat frequency phosphodiesterase |
| title | Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle |
| title_full | Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle |
| title_fullStr | Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle |
| title_full_unstemmed | Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle |
| title_short | Effects of Extracts and Flavonoids from <i>Drosera rotundifolia</i> L. on Ciliary Beat Frequency and Murine Airway Smooth Muscle |
| title_sort | effects of extracts and flavonoids from i drosera rotundifolia i l on ciliary beat frequency and murine airway smooth muscle |
| topic | <i>Drosera rotundifolia</i> antispasmodic trachea ciliary beat frequency phosphodiesterase |
| url | https://www.mdpi.com/1420-3049/27/19/6622 |
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