The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilization
Objective: To compare oocyte/embryo quality and treatmentoutcome in patients undergoing IVF/ICSI using recombinantluteinizing hormone (rhLH) or human menopausal gonadotrophin(hMG) as a supplement to recombinant follicle-stimulating hormone(rhFSH) during ovarian stimulation. Methods: After pituitaryd...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2005-06-01
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| Series: | Einstein (São Paulo) |
| Subjects: | |
| Online Access: | http://www.einstein.br/revista/biblioteca/artigos/vol3/num_2/Vol3_N2_P96.pdf |
| _version_ | 1819263531771494400 |
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| author | Paulo Marcelo Perin Mariangela Maluf Carlos Eduardo Czeresnia Patrícia Donadon Sousa |
| author_facet | Paulo Marcelo Perin Mariangela Maluf Carlos Eduardo Czeresnia Patrícia Donadon Sousa |
| author_sort | Paulo Marcelo Perin |
| collection | DOAJ |
| description | Objective: To compare oocyte/embryo quality and treatmentoutcome in patients undergoing IVF/ICSI using recombinantluteinizing hormone (rhLH) or human menopausal gonadotrophin(hMG) as a supplement to recombinant follicle-stimulating hormone(rhFSH) during ovarian stimulation. Methods: After pituitarydesensitization, sixty women undergoing their first IVF treatmentcycle were randomized to receive a fixed daily dose of either rhFSH(225 IU/d) plus hMG (75 IU/d) (group A, n=30) or rhLH (75 IU/d)(group B, n=30). The number/quality of metaphase II (MII) oocytes,zygote/embryo quality, and clinical pregnancy/implantation rateswere compared between the two groups. Results: The mean numberof normal/abnormal MII oocytes was 4.5±3.10/2.8±3.13 in groupA and 2.4±1.47/4.0±3.70 in group B, showing a significantdifference between groups (p=0.006; p=0.01; respectively). Thefertilization rate was similar in both groups, yet the number ofzygotes judged normal was significantly higher (p= 0.02) in groupA (2.4±2.40) as compared to group B (1.2±1.76). The mean numberand quality score of embryos at transfer were similar in both groups.Overall clinical pregnancy/implantation rates were 46.4%/25.3%in group A and 38.1%/17.1% in group B. The trend toward betterpregnancy outcomes among patients in group A did not reachstatistical significance. Conclusions: Our study suggests that theaddition of recombinant luteinizing hormone instead of humanmenopausal gonadotrophin to recombinant follicle-stimulatinghormone throughout ovulation induction in down-regulated womenundergoing IVF does not improve ovarian response and has anegative impact on oocyte/zygote quality. The result is a trendtoward poorer treatment outcome. |
| first_indexed | 2024-12-23T20:15:05Z |
| format | Article |
| id | doaj.art-e53775d323bb455aad3f67b7b12f399b |
| institution | Directory Open Access Journal |
| issn | 1679-4508 |
| language | English |
| last_indexed | 2024-12-23T20:15:05Z |
| publishDate | 2005-06-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-e53775d323bb455aad3f67b7b12f399b2022-12-21T17:32:44ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)1679-45082005-06-013296105The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilizationPaulo Marcelo PerinMariangela MalufCarlos Eduardo CzeresniaPatrícia Donadon SousaObjective: To compare oocyte/embryo quality and treatmentoutcome in patients undergoing IVF/ICSI using recombinantluteinizing hormone (rhLH) or human menopausal gonadotrophin(hMG) as a supplement to recombinant follicle-stimulating hormone(rhFSH) during ovarian stimulation. Methods: After pituitarydesensitization, sixty women undergoing their first IVF treatmentcycle were randomized to receive a fixed daily dose of either rhFSH(225 IU/d) plus hMG (75 IU/d) (group A, n=30) or rhLH (75 IU/d)(group B, n=30). The number/quality of metaphase II (MII) oocytes,zygote/embryo quality, and clinical pregnancy/implantation rateswere compared between the two groups. Results: The mean numberof normal/abnormal MII oocytes was 4.5±3.10/2.8±3.13 in groupA and 2.4±1.47/4.0±3.70 in group B, showing a significantdifference between groups (p=0.006; p=0.01; respectively). Thefertilization rate was similar in both groups, yet the number ofzygotes judged normal was significantly higher (p= 0.02) in groupA (2.4±2.40) as compared to group B (1.2±1.76). The mean numberand quality score of embryos at transfer were similar in both groups.Overall clinical pregnancy/implantation rates were 46.4%/25.3%in group A and 38.1%/17.1% in group B. The trend toward betterpregnancy outcomes among patients in group A did not reachstatistical significance. Conclusions: Our study suggests that theaddition of recombinant luteinizing hormone instead of humanmenopausal gonadotrophin to recombinant follicle-stimulatinghormone throughout ovulation induction in down-regulated womenundergoing IVF does not improve ovarian response and has anegative impact on oocyte/zygote quality. The result is a trendtoward poorer treatment outcome.http://www.einstein.br/revista/biblioteca/artigos/vol3/num_2/Vol3_N2_P96.pdfEmbryo implantationIn vitro fertilizationOocytesPregnancyLuteinizing hormoneZygote |
| spellingShingle | Paulo Marcelo Perin Mariangela Maluf Carlos Eduardo Czeresnia Patrícia Donadon Sousa The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilization Einstein (São Paulo) Embryo implantation In vitro fertilization Oocytes Pregnancy Luteinizing hormone Zygote |
| title | The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilization |
| title_full | The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilization |
| title_fullStr | The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilization |
| title_full_unstemmed | The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilization |
| title_short | The effect of recombinant human luteinizing hormone on oocyte/embryo quality and treatment outcome in down-regulated women undergoing in vitro fertilization |
| title_sort | effect of recombinant human luteinizing hormone on oocyte embryo quality and treatment outcome in down regulated women undergoing in vitro fertilization |
| topic | Embryo implantation In vitro fertilization Oocytes Pregnancy Luteinizing hormone Zygote |
| url | http://www.einstein.br/revista/biblioteca/artigos/vol3/num_2/Vol3_N2_P96.pdf |
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