Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activities
In this paper, a convenient method to fabricate flexible and free-standing surface-enhanced Raman scatting (SERS) substrates for direct bacteria detection without aptamer bonding is presented. SiO _2 nanofibers were prepared via electrospinning and calcination by using tetraethyl orthosilicate as th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2020-01-01
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| Series: | Materials Research Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2053-1591/abb8a0 |
| _version_ | 1797746301508517888 |
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| author | Menghui Wan Haodong Zhao Zhihua Wang Yanbao Zhao Lei Sun |
| author_facet | Menghui Wan Haodong Zhao Zhihua Wang Yanbao Zhao Lei Sun |
| author_sort | Menghui Wan |
| collection | DOAJ |
| description | In this paper, a convenient method to fabricate flexible and free-standing surface-enhanced Raman scatting (SERS) substrates for direct bacteria detection without aptamer bonding is presented. SiO _2 nanofibers were prepared via electrospinning and calcination by using tetraethyl orthosilicate as the precursor. Subsequently, it was coated with polydopamine (PDA) by self-polymerization. Finally, Ag@PDA@SiO _2 nanofibrous membranes were obtained through in situ growth of Ag nanoparticles in Tollens’ reagents. The as-prepared Ag@PDA@SiO _2 composite nanofibrous membranes were characterized by techniques of scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, energy-dispersive x-ray spectroscopy and thermo gravimetric analysis. The flexibility of the as-prepared nanofibrous membranes were verified simply through manual folding. Small molecule probes of 4-mercaptophenol (4-MPh) and 4-mercaptobenzoic acid (4-MBA) were chosen to investigate the SERS sensitivity of the as-prepared Ag@PDA@SiO _2 as free-standing substrates. Furthermore, Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), as typical strains of Gram-negative and Gram-positive bacteria, were performed to directly SERS detection by dropping bacteria suspension onto the fibrous membranes without any previous treatment, such as aptamer combination. In addition, the antimicrobial properties of the as-prepared Ag@PDA@SiO _2 electrospinning nanofibrous membranes were tested by inhibition zone and turbidity methods. The results show that Ag nanoparticles with an average diameter of 50 nm are uniformly deposited on the surface of electrospinning nanofibers, and the as-prepared Ag@PDA@SiO _2 nanofibrous membranes are flexible. As SERS substrates, it shows a rather high detection limitation of 10 ^−11 mol l ^−1 for 4-MPh and 4-MBA. More importantly, this substrate can be applied for bacteria label-free SERS detection, i.e., complicated procedures are avoided. Meanwihile, the as-prepared Ag@PDA@SiO _2 nanofibrous membranes exhibit excellent antibacterial properties. Thus, it has application prospects in trace bacteria detection and water purification. |
| first_indexed | 2024-03-12T15:34:52Z |
| format | Article |
| id | doaj.art-e538221a986148f9a1f554c889170e9d |
| institution | Directory Open Access Journal |
| issn | 2053-1591 |
| language | English |
| last_indexed | 2024-03-12T15:34:52Z |
| publishDate | 2020-01-01 |
| publisher | IOP Publishing |
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| series | Materials Research Express |
| spelling | doaj.art-e538221a986148f9a1f554c889170e9d2023-08-09T16:17:32ZengIOP PublishingMaterials Research Express2053-15912020-01-017909501210.1088/2053-1591/abb8a0Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activitiesMenghui Wan0Haodong Zhao1Zhihua Wang2Yanbao Zhao3Lei Sun4https://orcid.org/0000-0002-8112-2055Engineering Research Center for Nanomaterials, Henan University , Kaifeng 475004, People’s Republic of ChinaEngineering Research Center for Nanomaterials, Henan University , Kaifeng 475004, People’s Republic of ChinaHenan Engineering Research Center of Industrial Circulating Water Treatment, College of Chemistry and Chemical Engineering, Henan University , Kaifeng 475004, People’s Republic of ChinaEngineering Research Center for Nanomaterials, Henan University , Kaifeng 475004, People’s Republic of ChinaEngineering Research Center for Nanomaterials, Henan University , Kaifeng 475004, People’s Republic of ChinaIn this paper, a convenient method to fabricate flexible and free-standing surface-enhanced Raman scatting (SERS) substrates for direct bacteria detection without aptamer bonding is presented. SiO _2 nanofibers were prepared via electrospinning and calcination by using tetraethyl orthosilicate as the precursor. Subsequently, it was coated with polydopamine (PDA) by self-polymerization. Finally, Ag@PDA@SiO _2 nanofibrous membranes were obtained through in situ growth of Ag nanoparticles in Tollens’ reagents. The as-prepared Ag@PDA@SiO _2 composite nanofibrous membranes were characterized by techniques of scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, energy-dispersive x-ray spectroscopy and thermo gravimetric analysis. The flexibility of the as-prepared nanofibrous membranes were verified simply through manual folding. Small molecule probes of 4-mercaptophenol (4-MPh) and 4-mercaptobenzoic acid (4-MBA) were chosen to investigate the SERS sensitivity of the as-prepared Ag@PDA@SiO _2 as free-standing substrates. Furthermore, Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), as typical strains of Gram-negative and Gram-positive bacteria, were performed to directly SERS detection by dropping bacteria suspension onto the fibrous membranes without any previous treatment, such as aptamer combination. In addition, the antimicrobial properties of the as-prepared Ag@PDA@SiO _2 electrospinning nanofibrous membranes were tested by inhibition zone and turbidity methods. The results show that Ag nanoparticles with an average diameter of 50 nm are uniformly deposited on the surface of electrospinning nanofibers, and the as-prepared Ag@PDA@SiO _2 nanofibrous membranes are flexible. As SERS substrates, it shows a rather high detection limitation of 10 ^−11 mol l ^−1 for 4-MPh and 4-MBA. More importantly, this substrate can be applied for bacteria label-free SERS detection, i.e., complicated procedures are avoided. Meanwihile, the as-prepared Ag@PDA@SiO _2 nanofibrous membranes exhibit excellent antibacterial properties. Thus, it has application prospects in trace bacteria detection and water purification.https://doi.org/10.1088/2053-1591/abb8a0electrospinningSiO2 nanofibersAg nanoparticlesSERSbacteria detection |
| spellingShingle | Menghui Wan Haodong Zhao Zhihua Wang Yanbao Zhao Lei Sun Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activities Materials Research Express electrospinning SiO2 nanofibers Ag nanoparticles SERS bacteria detection |
| title | Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activities |
| title_full | Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activities |
| title_fullStr | Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activities |
| title_full_unstemmed | Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activities |
| title_short | Preparation of Ag@PDA@SiO2 electrospinning nanofibrous membranes for direct bacteria SERS detection and antimicrobial activities |
| title_sort | preparation of ag pda sio2 electrospinning nanofibrous membranes for direct bacteria sers detection and antimicrobial activities |
| topic | electrospinning SiO2 nanofibers Ag nanoparticles SERS bacteria detection |
| url | https://doi.org/10.1088/2053-1591/abb8a0 |
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